{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T11:16:11Z","timestamp":1780398971278,"version":"3.54.1"},"reference-count":28,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2004,10,1]],"date-time":"2004-10-01T00:00:00Z","timestamp":1096588800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:p>Craniosynostosis is a congenital disorder of premature ossification of cranial sutures, occurring in one of approximately every 2500 live human births. This work addressed a hypothesis that a cranial suture can be tissue-engineered from autologous cells. Dermal fibroblasts were isolated subcutaneously from growing rabbits, culture-expanded, and seeded in a gelatin scaffold. We fabricated a composite tissue construct by sandwiching the fibroblast-seeded gelatin scaffold between two collagen sponges loaded with recombinant human BMP2. Surgically created, full-thickness parietal defects were filled with the composite tissue construct in the same rabbits from which dermal fibroblasts had been obtained. After four-week in vivo implantation, there was de novo formation of tissue-engineered cranial suture, microscopically reminiscent of the adjacent natural cranial suture. The tissue-engineered cranial suture showed radiolucency on radiographic images, in contrast to radio-opacity of microscopically ossified calvarial defects filled with fibroblast-free, BMP2-loaded constructs. This approach may be refined for tissue engineering of cranial sutures for craniosynostosis patients.<\/jats:p>","DOI":"10.1177\/154405910408301003","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:39:42Z","timestamp":1231443582000},"page":"751-756","source":"Crossref","is-referenced-by-count":20,"title":["Tissue-engineered Rabbit Cranial Suture from Autologous Fibroblasts and                BMP2"],"prefix":"10.1177","volume":"83","author":[{"given":"L.","family":"Hong","sequence":"first","affiliation":[{"name":"Tissue Engineering Laboratory, Rm 237, Departments of Orthodontics,\r               Bioengineering, and Anatomy and Cell Biology, Univ. of Illinois at Chicago, MC 841,\r               801 South Paulina Street, Chicago, IL 60612-7211, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.J.","family":"Mao","sequence":"additional","affiliation":[{"name":"Tissue Engineering Laboratory, Rm 237, Departments of Orthodontics,\r               Bioengineering, and Anatomy and Cell Biology, Univ. of Illinois at Chicago, MC 841,\r               801 South Paulina Street, Chicago, IL 60612-7211, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2004,10,1]]},"reference":[{"key":"BRADLEY-ETAL-2000","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-200006000-00017"},{"key":"COHEN-ETAL-2000","unstructured":"Cohen MM Jr, MacLean RE, editors\n               -2000- The biology of sutures. In: Craniosynostosis: diagnosis,\n               evaluation, and management. New York: Raven Press, pp. 81\u2013103."},{"key":"COLLINS-ETAL-2004","unstructured":"Collins JM, Ramamoorthy K, Da Silveira A, Patston\n               PA, Mao JJ -2004- Microstrain in intramembranous bones induces altered\n               gene expression of MMP1 and MMP2 in the rat. 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New York: Oxford Unviersity Press, pp.\n               228\u2013268."},{"key":"WARREN-AND-LONGAKER-2001","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.2001.42.6.646"},{"key":"WILKIE-AND-MORRISS-KAY-2001","doi-asserted-by":"publisher","DOI":"10.1038\/35076601"},{"key":"YEN-ETAL-1989","doi-asserted-by":"publisher","DOI":"10.1177\/00220345890680120501"},{"key":"ZIMMERMAN-ETAL-1998","doi-asserted-by":"publisher","DOI":"10.1007\/s004290050127"}],"container-title":["Journal of Dental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/154405910408301003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/154405910408301003","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/154405910408301003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:00:34Z","timestamp":1777496434000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/154405910408301003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,10]]},"references-count":28,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2004,10]]}},"alternative-id":["10.1177\/154405910408301003"],"URL":"https:\/\/doi.org\/10.1177\/154405910408301003","relation":{},"ISSN":["0022-0345","1544-0591"],"issn-type":[{"value":"0022-0345","type":"print"},{"value":"1544-0591","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,10]]}}}