{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T12:24:50Z","timestamp":1777811090950,"version":"3.51.4"},"reference-count":21,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2005,4,1]],"date-time":"2005-04-01T00:00:00Z","timestamp":1112313600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:p>Gingival fibroblasts actively accumulate tetracyclines, thereby enhancing their redistribution from blood to gingiva. Since growth factors and pro-inflammatory cytokines regulate many fibroblast activities, they could potentially enhance fibroblast minocycline accumulation. To test this hypothesis, we treated gingival fibroblast monolayers for 1 or 6 hours with platelet-derived growth factor-BB (PDGF), fibroblast growth factor-2 (FGF), transforming growth factor-\u03b21 (TGF), or tumor necrosis factor-\u03b1 (TNF). Minocycline uptake was assayed at 37\u00b0 by a fluorescence method. All 4 factors significantly enhanced minocycline uptake (P \u2264 0.008, ANOVA), primarily by increasing the affinity of transport. Treatment for 6 hours with 10 ng\/mL FGF, PDGF, TGF, or TNF enhanced fibroblast minocycline uptake by 19% to 25%. Phorbol myristate acetate enhanced fibroblast minocycline uptake by 28%, suggesting that protein kinase C plays a role in up-regulating transport. These effects on transport provide a mechanism by which systemic tetracyclines could be preferentially distributed to gingival wound or inflammatory sites.<\/jats:p>","DOI":"10.1177\/154405910508400405","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:39:42Z","timestamp":1231443582000},"page":"320-323","source":"Crossref","is-referenced-by-count":7,"title":["Modulation of Gingival Fibroblast Minocycline Accumulation by Biological Mediators"],"prefix":"10.1177","volume":"84","author":[{"given":"J.D.","family":"Walters","sequence":"first","affiliation":[{"name":"Section of Periodontology, College of Dentistry, The Ohio State University Health Sciences Center, 305 West 12th Avenue, PO Box 182357, Columbus, OH 43218-2357, USA;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.J.","family":"Nakkula","sequence":"additional","affiliation":[{"name":"Section of Periodontology, College of Dentistry, The Ohio State University Health Sciences Center, 305 West 12th Avenue, PO Box 182357, Columbus, OH 43218-2357, USA;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Maney","sequence":"additional","affiliation":[{"name":"Section of Periodontology, College of Dentistry, The Ohio State University Health Sciences Center, 305 West 12th Avenue, PO Box 182357, Columbus, OH 43218-2357, USA;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2005,4,1]]},"reference":[{"key":"AXMANN-ETAL-1998","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.9188"},{"key":"BIRKEDAL-HANSEN-1993","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1993.tb02113.x"},{"key":"BRADFORD-1976","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"CHRISTERSSON-ETAL-1987","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1987.58.8.529"},{"key":"CIANCIO-ETAL-1980","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1980.51.9.530"},{"key":"CLARK-1996","unstructured":"Clark RAF -1996- The molecular and cellular biology of wound repair. 2nd ed. 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