{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:10:40Z","timestamp":1792627840031,"version":"4.5.0"},"reference-count":27,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2005,8,1]],"date-time":"2005-08-01T00:00:00Z","timestamp":1122854400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:p>\n                    Various kinds of chemical mediators are synthesized in the course of pulpitis; thus, control of their production would assist in inducing a reduction in pulpal inflammation. We hypothesized that nitric oxide (NO) would be an important mediator of pulpal inflammation. Pulpal inflammation was induced by the application of LPS in rat incisor pulp, and inducible nitric oxide synthase (iNOS) expression was evaluated by reverse-transcription\/polymerase chain-reaction and immunohistochemical staining. After LPS application, iNOS mRNA was first detected after 3 hrs, peaked at 6 hrs, and decreased thereafter. iNOS-positive cells were macrophages and neutrophils. An NOS inhibitor caused drastic decreases in the expression of pro-inflammatory cytokines and COX2 mRNA, which was highly induced in the LPS-induced pulpitis. These results indicate that NO synthesis is related to the initiation of mediator production, and that its down-regulation should contribute to the prevention of pro-inflammatory mediator synthesis. Abbreviations: ANOVA, analysis of variance; COX2, cyclo-oxygenase 2; EDTA, ethylenediaminetetraacetic acid; iNOS, inducible nitric oxide synthase; IL, interleukin; L-NAME, N\n                    <jats:sup>G<\/jats:sup>\n                    -nitro L-arginine methyl ester; LPS, lipopolysaccharide; NO, nitric oxide; NOS, nitric oxide synthases; PG, prostaglandin; RT-PCR, reverse-transcription\/polymerase chain-reaction; TNF\u03b1, tumor necrosis factor alpha.\n                  <\/jats:p>","DOI":"10.1177\/154405910508400815","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:39:42Z","timestamp":1231443582000},"page":"762-767","source":"Crossref","is-referenced-by-count":53,"title":["Effect of NOS Inhibitor on Cytokine and COX2 Expression in Rat Pulpitis"],"prefix":"10.1177","volume":"84","author":[{"given":"N.","family":"Kawashima","sequence":"first","affiliation":[{"name":"Pulp Biology and Endodontics, Department of Restorative Sciences,"},{"name":"Molecular Pathology, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical & Dental University, and"},{"name":"Center of Excellence -COE- Program for Research on Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical & Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Nakano-Kawanishi","sequence":"additional","affiliation":[{"name":"Pulp Biology and Endodontics, Department of Restorative Sciences,"},{"name":"Molecular Pathology, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical & Dental University, and"},{"name":"Center of Excellence -COE- Program for Research on Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical & Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Pulp Biology and Endodontics, Department of Restorative Sciences,"},{"name":"Molecular Pathology, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical & Dental University, and"},{"name":"Center of Excellence -COE- Program for Research on Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical & Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Takagi","sequence":"additional","affiliation":[{"name":"Pulp Biology and Endodontics, Department of Restorative Sciences,"},{"name":"Molecular Pathology, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical & Dental University, and"},{"name":"Center of Excellence -COE- Program for Research on Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical & Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Suda","sequence":"additional","affiliation":[{"name":"Pulp Biology and Endodontics, Department of Restorative Sciences,"},{"name":"Molecular Pathology, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical & Dental University, and"},{"name":"Center of Excellence -COE- Program for Research on Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical & Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2005,8,1]]},"reference":[{"key":"AONO-ETAL-1997","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19970601)65:3<349::AID-JCB5>3.0.CO;2-S"},{"key":"COLON-ETAL-2000","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2000.02047.x"},{"key":"DE-MELLO-ETAL-1997","doi-asserted-by":"publisher","DOI":"10.1007\/s000110050086"},{"key":"DI-NARDO-ETAL-2004","doi-asserted-by":"publisher","DOI":"10.1177\/154405910408300408"},{"key":"HILL-ETAL-1996","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.37.22672"},{"key":"HOSOYA-AND-MATSUSHIMA-1997","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-2399(97)80205-1"},{"key":"KANKURI-ETAL-2001","doi-asserted-by":"crossref","unstructured":"Kankuri E, Vaali K, Knowles RG, Lahde M, Korpela R, Vapaatalo H,\n                      et al.\n                      -2001- Suppression of acute experimental colitis by a highly selective inducible nitric-oxide synthase inhibitor, N-[3-aminomethyl-benzyl]acetamidine. 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