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To evaluate the osteogenic potential of PDL cells, we applied a 12% uni-axial cyclic tensile strain to cultured human PDL cells and analyzed the differential expression of 78 genes implicated in osteoblast differentiation and bone metabolism by real-time RT-PCR array technology. Sixteen genes showed statistically significant changes in expression in response to alterations in their mechanical environment, including cell adhesion molecules and collagen fiber types. Genes linked to the osteoblast phenotype that were up-regulated included BMP2, BMP6, ALP, SOX9, MSX1, and VEGFA; those down-regulated included BMP4 and EGF. This study has expanded our knowledge of the transcriptional profile of PDL cells and identified several new mechanoresponsive genes.<\/jats:p>","DOI":"10.1177\/154405910708601214","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:39:42Z","timestamp":1231443582000},"page":"1212-1216","source":"Crossref","is-referenced-by-count":117,"title":["Osteogenic Gene Expression by Human Periodontal Ligament Cells under Cyclic Tension"],"prefix":"10.1177","volume":"86","author":[{"given":"D.C.","family":"Wescott","sequence":"first","affiliation":[{"name":"Department of Oral Sciences, Faculty of Dentistry, University of Otago, PO Box 647, Dunedin, New Zealand;"},{"name":"Department of Zoology, Division of Sciences, University of Otago, Dunedin, New Zealand; and"},{"name":"Faculty of Dentistry, National University of Singapore, Level 3 National University Hospital, 5 Lower Kent Ridge Road, Singapore 119074"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.N.","family":"Pinkerton","sequence":"additional","affiliation":[{"name":"Department of Oral Sciences, Faculty of Dentistry, University of Otago, PO Box 647, Dunedin, New Zealand;"},{"name":"Department of Zoology, Division of Sciences, University of Otago, Dunedin, New Zealand; and"},{"name":"Faculty of Dentistry, National University of Singapore, Level 3 National University Hospital, 5 Lower Kent Ridge Road, Singapore 119074"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.J.","family":"Gaffey","sequence":"additional","affiliation":[{"name":"Department of Oral Sciences, Faculty of Dentistry, University of Otago, PO Box 647, Dunedin, New Zealand;"},{"name":"Department of Zoology, Division of Sciences, University of Otago, Dunedin, New Zealand; and"},{"name":"Faculty of Dentistry, National University of Singapore, Level 3 National University Hospital, 5 Lower Kent Ridge Road, Singapore 119074"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.T.","family":"Beggs","sequence":"additional","affiliation":[{"name":"Department of Oral Sciences, Faculty of Dentistry, University of Otago, PO Box 647, Dunedin, New Zealand;"},{"name":"Department of Zoology, Division of Sciences, University of Otago, Dunedin, New Zealand; and"},{"name":"Faculty of Dentistry, National University of Singapore, Level 3 National University Hospital, 5 Lower Kent Ridge Road, Singapore 119074"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.J.","family":"Milne","sequence":"additional","affiliation":[{"name":"Department of Oral Sciences, Faculty of Dentistry, University of Otago, PO Box 647, Dunedin, New Zealand;"},{"name":"Department of Zoology, Division of Sciences, University of Otago, Dunedin, New Zealand; and"},{"name":"Faculty of Dentistry, National University of Singapore, Level 3 National University Hospital, 5 Lower Kent Ridge Road, Singapore 119074"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.C.","family":"Meikle","sequence":"additional","affiliation":[{"name":"Department of Oral Sciences, Faculty of Dentistry, University of Otago, PO Box 647, Dunedin, New Zealand;"},{"name":"Department of Zoology, Division of Sciences, University of Otago, Dunedin, New Zealand; and"},{"name":"Faculty of Dentistry, National University of Singapore, Level 3 National University Hospital, 5 Lower Kent Ridge Road, Singapore 119074"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2007,12,1]]},"reference":[{"key":"BARAITSER-AND-WINTER-2001","unstructured":"Baraitser M, Winter RM -2001- London dysmorphology database, London neurogenetics database & dysmorphology photo library on CD-ROM. 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