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Since vascularization is critical for organogenesis and tissue engineering, this work aimed to study: (a) blood vessel formation during tooth development, (b) the fate of blood vessels in cultured teeth and re-associations, and (c) vascularization after in vivo implantation. Ex vivo, blood vessels developed in the dental mesenchyme from the cap to bell stages and in the enamel organ, shortly before ameloblast differentiation. In cultured teeth and re-associations, blood-vessel-like structures remained in the peridental mesenchyme, but never developed into dental tissues. After implantation, both teeth and re-associations became revascularized, although later in the case of the re-associations. In implanted re-associations, newly formed blood vessels originated from the host, allowing for their survival, and affording conditions organ growth, mineralization, and enamel secretion.<\/jats:p>","DOI":"10.1177\/154405910808701216","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:39:42Z","timestamp":1231443582000},"page":"1138-1143","source":"Crossref","is-referenced-by-count":79,"title":["Vascularization of Engineered Teeth"],"prefix":"10.1177","volume":"87","author":[{"given":"A.","family":"Nait Lechguer","sequence":"first","affiliation":[{"name":"INSERM UMR 595, Facult\u00e9 de M\u00e9decine, 11, rue Humann, 67085 Strasbourg cedex, France;"},{"name":"Dental School, Universit\u00e9 Louis Pasteur, Strasbourg, France; and"},{"name":"International Collaborating Centre in Orofacial Genetics and Development, University of Liverpool, School of Dental Sciences, Liverpool, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Kuchler-Bopp","sequence":"additional","affiliation":[{"name":"INSERM UMR 595, Facult\u00e9 de M\u00e9decine, 11, rue Humann, 67085 Strasbourg cedex, France;"},{"name":"Dental School, Universit\u00e9 Louis Pasteur, Strasbourg, France; and"},{"name":"International Collaborating Centre in Orofacial Genetics and Development, University of Liverpool, School of Dental Sciences, Liverpool, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Hu","sequence":"additional","affiliation":[{"name":"INSERM UMR 595, Facult\u00e9 de M\u00e9decine, 11, rue Humann, 67085 Strasbourg cedex, France;"},{"name":"Dental School, Universit\u00e9 Louis Pasteur, Strasbourg, France; and"},{"name":"International Collaborating Centre in Orofacial Genetics and Development, University of Liverpool, School of Dental Sciences, Liverpool, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Ha\u00efkel","sequence":"additional","affiliation":[{"name":"INSERM UMR 595, Facult\u00e9 de M\u00e9decine, 11, rue Humann, 67085 Strasbourg cedex, France;"},{"name":"Dental School, Universit\u00e9 Louis Pasteur, Strasbourg, France; and"},{"name":"International Collaborating Centre in Orofacial Genetics and Development, University of Liverpool, School of Dental Sciences, Liverpool, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Lesot","sequence":"additional","affiliation":[{"name":"INSERM UMR 595, Facult\u00e9 de M\u00e9decine, 11, rue Humann, 67085 Strasbourg cedex, France;"},{"name":"Dental School, Universit\u00e9 Louis Pasteur, Strasbourg, France; and"},{"name":"International Collaborating Centre in Orofacial Genetics and Development, University of Liverpool, School of Dental Sciences, Liverpool, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2008,12,1]]},"reference":[{"key":"AHMAD-AND-RUCH-1987","doi-asserted-by":"crossref","unstructured":"Ahmad N, Ruch JV -1987- Comparison of growth and cell proliferation kinetics during mouse molar odontogenesis\n                      in vivo\n                      and\n                      in vitro\n                      . 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