{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T01:03:46Z","timestamp":1777943026775,"version":"3.51.4"},"reference-count":103,"publisher":"SAGE Publications","issue":"21-22","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100000031","name":"Institute of Human Development, Child and Youth Health","doi-asserted-by":"crossref","award":["202203PJT-480366-CA2-CEEA-58792"],"award-info":[{"award-number":["202203PJT-480366-CA2-CEEA-58792"]}],"id":[{"id":"10.13039\/501100000031","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","award":["5R01HD100179-02"],"award-info":[{"award-number":["5R01HD100179-02"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells and Development"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:p>\n                    Cannabis use during pregnancy is increasing. In rodent models of delta-9 tetrahydrocannabinol (\u03949-THC) exposure during pregnancy, placental pathology, including compromised labyrinth development, is reported. Cannabinoid receptor 1 (CB1\/\n                    <jats:italic toggle=\"yes\">Cnr1<\/jats:italic>\n                    ) is the primary mediator of \u03949-THC action, with its expression reportedly limited to the placental junctional zone in the rodent. Given a \u03949-THC-induced labyrinth-specific pathology, we predicted more diverse expression. This study aimed to elucidate the spatiotemporal expression of CB1\/\n                    <jats:italic toggle=\"yes\">Cnr1<\/jats:italic>\n                    in the rodent and assess whether it mediates \u03949-THC-induced alterations in trophoblast differentiation. Results revealed CB1 expression in all maternal blood-facing trophoblast cells. Furthermore, \u03949-THC exposure (at levels matching those reported in maternal serum) had a more significant effect on the expression of markers associated with differentiating trophoblast cells than on proliferating trophoblast stem (TS) cells. \u03949-THC impacted mouse (m) TS cell differentiation in a CB1-dependent manner, reducing the expression of syncytiotrophoblast (SynT) markers, driving differentiation along the junctional zone\/trophoblast giant cell pathway. mTS cells without\n                    <jats:italic toggle=\"yes\">Cnr1\/<\/jats:italic>\n                    CB1 (mTS\n                    <jats:italic toggle=\"yes\">\n                      <jats:sup>Cnr1<\/jats:sup>\n                    <\/jats:italic>\n                    <jats:sup>KO<\/jats:sup>\n                    ) did not express markers of SynT cells or the differentiated junctional zone cell types. However, at a higher than physiologically relevant concentration, \u03949-THC (15 \u03bcM) induced\n                    <jats:italic toggle=\"yes\">Gcm1<\/jats:italic>\n                    (SynT) expression in mTS\n                    <jats:italic toggle=\"yes\">\n                      <jats:sup>Cnr1<\/jats:sup>\n                    <\/jats:italic>\n                    <jats:sup>KO<\/jats:sup>\n                    cells. This study reveals a mechanism by which \u03949-THC may impact placental growth.\n                  <\/jats:p>","DOI":"10.1177\/15473287251392544","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T06:07:54Z","timestamp":1762409274000},"page":"441-455","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Delta 9-Tetrahydrocannabinol Signaling Through Cannabinoid Receptor 1 Alters Trophoblast Differentiation"],"prefix":"10.1177","volume":"34","author":[{"given":"Jessica L.","family":"Koven","sequence":"first","affiliation":[{"name":"Department of Biomedical and Molecular Sciences, Queen\u2019s University, Kingston, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryony V.","family":"Natale","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Molecular Sciences, Queen\u2019s University, Kingston, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel B.","family":"Hardy","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology, The University of Western Ontario, London, Ontario, Canada."},{"name":"The Children\u2019s Health Research Institute, The University of Western Ontario, London, Ontario, Canada."},{"name":"Lawson Health Research Institute, The University of Western Ontario, London, Ontario, Canada."},{"name":"Department of Obstetrics and Gynaecology, The University of Western Ontario, London, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David R.C.","family":"Natale","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Molecular Sciences, Queen\u2019s University, Kingston, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,11,5]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-024-10793-5"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5396.2072"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.12.038"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.112439"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2017.11.004"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells13060486"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-83563-9"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.14476"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu7010360"},{"key":"e_1_3_3_11_2","first-page":"98","article-title":"Cannabis use during pregnancy and postpartum","volume":"66","author":"Badowski S","year":"2020","unstructured":"Badowski S, , Smith G. Cannabis use during pregnancy and postpartum. Can Fam Physician, 2020; 66:98\u2013103.","journal-title":"Can Fam Physician"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2023.03.002"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(89)90380-9"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/embj.201488086"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0002-9378(99)70583-1"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2020.104822"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.0951-05.2005"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0707442"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-57318-6"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.4199\/C00016ED1V01Y201008ISP009"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2015.06.005"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/510540"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2019-00024"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpsc.2020.07.016"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2020.101301"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3565(25)24023-X"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1010892107"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2319"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-10-19"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00870.x"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2022.0290"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-35606-x"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-06008-2"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1089\/can.2023.0166"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2025.02.002"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.381145"},{"key":"e_1_3_3_38_2","volume-title":"Autoimmunity: From Bench to Bedside","author":"Arango M","year":"2013","unstructured":"Arango M, , Quintero-Ronderos P, , Castiblanco J, et al. Cell culture and cell analysis. In: Autoimmunity: From Bench to Bedside. (JM Anaya, , Y S, , A Rojas-Villarraga ed.) El Rosario University Press: Bogota, Colombia; 2013."},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2018.4841"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2015.238287"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/16.5.283"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/28.6.394"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-008-1260-2"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-03242-7"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2019.04.008"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2011.162958"},{"key":"e_1_3_3_48_2","unstructured":"Hellemans J Vandesompele J Clarey M et al. PrimePCRTM assays: Meeting the MIQE guidelines by full wet-lab validation. Hercules CA; 2012. Available from: https:\/\/commerce.bio-rad.com\/webroot\/web\/pdf\/lsr\/global\/english\/primePCR\/PrimePCR_Assay_Validation_Tech_Note_6262.pdf"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-41158-0"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.268821.115"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2013.10.019"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.020099"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-22040-2"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.01.009"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2016517118"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-352"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2014.12.002"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2015.05.013"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells1041197"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.07.032"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.03.040"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.133462"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjy054"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2012.05.036"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-1799"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004-0388"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2014.10.011"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12884-014-0395-x"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2009.03.015"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2023.21146"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1097\/aog.0000000000002354"},{"key":"e_1_3_3_72_2","unstructured":"Renard JK Sarah. Clearing the smoke on cannabis: cannabis use during pregnancy and breastfeeding an update. Ottawa Canada. 2022. https:\/\/www.ccsa.ca\/en\/clearing-smoke-cannabis-cannabis-use-during-pregnancy-and-breastfeeding"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1165-5"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2023.153614"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2024.55310"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1159\/000320550"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1186\/s42238-023-00184-x"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2203071119"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.1038\/77076"},{"key":"e_1_3_3_80_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001536"},{"key":"e_1_3_3_81_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0902925106"},{"key":"e_1_3_3_82_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008055"},{"key":"e_1_3_3_83_2","doi-asserted-by":"publisher","DOI":"10.1161\/hypertensionaha.121.17267"},{"key":"e_1_3_3_84_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2017.10.014"},{"key":"e_1_3_3_85_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-025-04487-z"},{"key":"e_1_3_3_86_2","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2012.0596"},{"key":"e_1_3_3_87_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-458-2_14"},{"key":"e_1_3_3_88_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26175413"},{"key":"e_1_3_3_89_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2018.0227"},{"key":"e_1_3_3_90_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.478354"},{"key":"e_1_3_3_91_2","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.00176"},{"key":"e_1_3_3_92_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950190208"},{"key":"e_1_3_3_93_2","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.1092543"},{"key":"e_1_3_3_94_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.143.7.1997"},{"key":"e_1_3_3_95_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.12.12.4066"},{"key":"e_1_3_3_96_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0014-5793(00)02295-x"},{"key":"e_1_3_3_97_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780362"},{"key":"e_1_3_3_98_2","volume-title":"Reference Module in Biomedical Sciences","author":"Natale BV","year":"2018","unstructured":"Natale BV, , Natale DR. Placentation in the Mouse. In: Reference Module in Biomedical Sciences. Elsevier; 2018."},{"key":"e_1_3_3_99_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.88795"},{"key":"e_1_3_3_100_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.3609"},{"key":"e_1_3_3_101_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2009.05.015"},{"key":"e_1_3_3_102_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-62514-w"},{"key":"e_1_3_3_103_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2310502121"},{"key":"e_1_3_3_104_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2016.05.020"}],"container-title":["Stem Cells and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15473287251392544","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/15473287251392544","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15473287251392544","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T07:13:32Z","timestamp":1777706012000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/15473287251392544"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":103,"journal-issue":{"issue":"21-22","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1177\/15473287251392544"],"URL":"https:\/\/doi.org\/10.1177\/15473287251392544","relation":{},"ISSN":["1547-3287","1557-8534"],"issn-type":[{"value":"1547-3287","type":"print"},{"value":"1557-8534","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11]]}}}