{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T17:32:55Z","timestamp":1793208775747,"version":"4.5.0"},"reference-count":16,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Guangxi University of Chinese Medicine","award":["S202413643002, S202513643087,S202510600092"],"award-info":[{"award-number":["S202413643002, S202513643087,S202510600092"]}],"id":[{"id":"https:\/\/ror.org\/024v0gx67","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Natural Science Foundation of China","award":["32560167"],"award-info":[{"award-number":["32560167"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells and Development"],"abstract":"\n Bone marrow-derived leptin receptor-expressing (LepR\n +<\/jats:sup>\n ) stromal cells (LepR\n +<\/jats:sup>\n BMSCs) have been identified as the primary source of osteoblasts, adipocytes, and fibroblasts in the bone marrow, and they play key roles in the maintenance of the bone marrow microenvironment; however, research on the fate and immunomodulatory changes of human LepR\n +<\/jats:sup>\n BMSCs is limited. Here, we isolated LepR\n +<\/jats:sup>\n BMSCs from a young donor and studied their fate changes of osteogenic, adipogenic, myogenic differentiation, and immunomodulation with or without CXM102, a novel compound that we have previously demonstrated its anti-osteoporotic effects in mice. Our results showed that CXM102 facilitated a preferential lineage shift toward osteogenesis from adipogenesis of LepR\n +<\/jats:sup>\n BMSCs\n in vitro<\/jats:italic>\n , as evidenced by increased\n RUNX 2<\/jats:italic>\n expression and decreased\n PPAR\u03b3<\/jats:italic>\n in a bivalent differentiation system. Moreover, interleukin-6 (IL-6), interleukin-1\u03b2 (IL-1\u03b2), and tumor necrosis factor-\u03b1 (TNF-\u03b1) were markedly downregulated in HS-5 stromal cells cocultured with conditioned medium from CXM102-treated LepR\n +<\/jats:sup>\n BMSCs. Furthermore, CXM102 also inhibited collagen I deposition in LepR\n +<\/jats:sup>\n BMSCs when induced into myogenesis. These results demonstrated that CXM102 could promote osteogenesis, but inhibit adipogenesis and myogenesis of LepR\n +<\/jats:sup>\n BMSCs, while also showing benign immunomodulation\n in vitro<\/jats:italic>\n .\n <\/jats:p>","DOI":"10.1177\/15473287261497533","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T04:56:51Z","timestamp":1792126611000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["CXM102 Regulates the Multiple Differentiation and Anti-Inflammation of Human Bone Marrow-Derived Leptin Receptor-Positive Stromal Cells\n In Vitro<\/i>"],"prefix":"10.1177","author":[{"given":"Dawei","family":"Qiu","sequence":"first","affiliation":[{"name":"Department of Physical Education, Guangxi University of Chinese Medicine, Nanning, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junmo","family":"Chen","sequence":"additional","affiliation":[{"name":"Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, Nanning, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiran","family":"Dai","sequence":"additional","affiliation":[{"name":"Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, Nanning, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongliang","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanghua","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, Nanning, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1208-7847","authenticated-orcid":false,"given":"Ziwei","family":"Luo","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine, Institute of Traditional Chinese and Zhuang-Yao Ethnic Medicine, Guangxi University of Chinese Medicine, Nanning, China."},{"name":"Guangxi Engineering Research Center for High-Value Utilization of Guangxi-Produced Authentic Medicinal Herbs, Nanning, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,10,15]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells13110910"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2014.06.008"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2021108415"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2025.104749"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022018212"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1104-8"},{"key":"e_1_3_2_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.15211"},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3530"},{"key":"e_1_3_2_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mex.2024.102637"},{"key":"e_1_3_2_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-024-00351-7"},{"key":"e_1_3_2_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V85.4.997.bloodjournal854997"},{"key":"e_1_3_2_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.221"},{"key":"e_1_3_2_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-363-3_7"},{"key":"e_1_3_2_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-025-02313-9"},{"key":"e_1_3_2_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-018-0023-5"},{"key":"e_1_3_2_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2023.0229"}],"container-title":["Stem 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