{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T01:29:04Z","timestamp":1788571744483,"version":"build-2803163510"},"reference-count":42,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:00:00Z","timestamp":1776902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Research Project of the Heilongjiang Provincial Health Commission","award":["20240303010404"],"award-info":[{"award-number":["20240303010404"]}]},{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"crossref","award":["PL2024H213"],"award-info":[{"award-number":["PL2024H213"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Rejuvenation Research"],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:p>\n                    To elucidate the mechanism of action of the\n                    <jats:italic toggle=\"yes\">Astragalus membranaceus<\/jats:italic>\n                    \u2013\n                    <jats:italic toggle=\"yes\">Panax ginseng<\/jats:italic>\n                    herb pair in treating coronary heart disease (CHD) through network pharmacology combined with molecular docking and experimental validation. Active ingredients and target proteins were retrieved from the Traditional Chinese Medicine Systems Pharmacology database. CHD-related targets were obtained from GeneCards and DrugBank. Overlapping targets were identified with a Venn diagram, and a protein\u2013protein interaction network was built in STRING. Core targets were screened via topological analysis in Cytoscape 3.9.0. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment were performed in Metascape. AutoDock Vina was used for molecular docking.\n                    <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                    , tumor necrosis factor (TNF)-\u03b1 levels in HL-1 cells subjected to oxygen-glucose deprivation (OGD) were measured by ELISA, and the effect of mangiferin pretreatment on cell viability was assessed with a CCK-8 assay. Forty-one active ingredients and 207 putative targets were identified for the herbal pair, and 1843 CHD-related targets were collected; 67 overlapping therapeutic targets were obtained. Topological analysis yielded 27 core targets. GO terms were enriched in responses to lipopolysaccharide, toxic substances, and organic cyclic compounds; molecular functions included cytokine-receptor binding and transcription-factor binding; and cellular components were mainly membrane rafts and extracellular matrix. KEGG analysis highlighted the TNF, fluid-shear-stress, and NF-\u03baB signaling pathways. Docking showed strong binding between key components and targets.\n                    <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                    experiments demonstrated that formononetin pretreatment significantly reduced OGD-induced TNF-\u03b1 levels and improved HL-1 cell viability. The\n                    <jats:italic toggle=\"yes\">A. membranaceus<\/jats:italic>\n                    \u2013\n                    <jats:italic toggle=\"yes\">P. ginseng<\/jats:italic>\n                    combination herb pair exerts anti-CHD effects via multiple bioactive ingredients (\n                    <jats:italic toggle=\"yes\">e.g.,<\/jats:italic>\n                    formononetin, quercetin, isorhamnetin, ginsenoside Rh2, kaempferol) by targeting TNF, AKT1, PTGS2, and JUN and modulating pathways including the TNF and NF-\u03baB signaling pathways.\n                  <\/jats:p>","DOI":"10.1177\/15491684261446288","type":"journal-article","created":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T11:59:22Z","timestamp":1776945562000},"page":"264-272","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Elucidating the Mechanism of Action of the Astragalus\u2013Ginseng Herb Pair in Treating Coronary Heart Disease: A Network Pharmacology and Experimental Validation Approach"],"prefix":"10.1177","volume":"29","author":[{"given":"Zhiping","family":"Liu","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Chen","sequence":"additional","affiliation":[{"name":"Heilongjiang University of Chinese Medicine, Harbin, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianwei","family":"Meng","sequence":"additional","affiliation":[{"name":"Heilongjiang University of Chinese Medicine, Harbin, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Heilongjiang University of Chinese Medicine, Harbin, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengjia","family":"Li","sequence":"additional","affiliation":[{"name":"Heilongjiang University of Chinese Medicine, Harbin, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changxing","family":"Liu","sequence":"additional","affiliation":[{"name":"Heilongjiang University of Chinese Medicine, Harbin, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7133-6751","authenticated-orcid":false,"given":"Yingying","family":"Wang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,4,23]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2014.04.015"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14020319"},{"issue":"09","key":"e_1_3_5_4_1","first-page":"22","article-title":"The effect of the Yiquan Yang and blood-activating formula on the t-wave-to-q-wave interval and prognosis following revascularisation in elderly patients with Acute Myocardial Infarction","volume":"28","author":"Geng L","year":"2015","unstructured":"3.Geng L, , Li K. The effect of the Yiquan Yang and blood-activating formula on the t-wave-to-q-wave interval and prognosis following revascularisation in elderly patients with Acute Myocardial Infarction. Raditional Chinese Med Res 2015;28(09):22\u201324.","journal-title":"Raditional Chinese Med Res"},{"issue":"08","key":"e_1_3_5_5_1","first-page":"104","article-title":"Research progress on pharmacological action and clinical application of combination of Radix Astragalus and Radix Ginseng","volume":"51","author":"Wanying W","year":"2023","unstructured":"4.Wanying W, , Siliang J, , Junhong C, , Yonggang X, , Jun L. Research progress on pharmacological action and clinical application of combination of Radix Astragalus and Radix Ginseng. 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