{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T01:06:41Z","timestamp":1777943201343,"version":"3.51.4"},"reference-count":43,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2015,2,20]],"date-time":"2015-02-20T00:00:00Z","timestamp":1424390400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Surg Innov"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>Background. The treatment of complex fistulae-in-ano is challenging and often includes a number of operations due to high rates of recurrence. Recently, techniques using in vitro expanded adipose tissue\u2013derived stem cells have been described. We describe a novel treatment for cryptoglandular fistulae used in 7 patients, using a combination of surgical closure of the internal opening and real-time autologous adipose tissue\u2013derived regenerative cells (ADRC)\u2013enhanced lipofilling, without need for in vitro expansion. Methods. Following exclusion of active perianal sepsis, patients underwent a standard tumescent liposuction procedure, harvesting ~300 to 400 mL of raw lipoaspirate. The lipoaspirate was prepared in real time, using the Celution 800\/CRS system to obtain the stromal vascular fraction containing ADRCs. After excision of the fistula tract and closure of the internal orifice, fresh ADRC-enhanced lipoaspirate was injected into and around the fistula tract. Results. At 6-months\u2019 follow-up, 5 of 7 (71.4%) patients showed clinical signs of fistula closure; one of these patients had a recurrence at 10 months due to sepsis. The remaining 4 patients (57.1%) all had complete fistula closure at a median of 46 months\u2019 follow-up. There were no adverse events associated with the technique, and no new incontinence. Conclusion. Treatment of cryptoglandular fistulae-in-ano with ADRC-enhanced lipofilling appears feasible and safe, and may add to the range of procedures that can be used to treat this difficult problem.<\/jats:p>","DOI":"10.1177\/1553350615572656","type":"journal-article","created":{"date-parts":[[2015,2,26]],"date-time":"2015-02-26T02:32:17Z","timestamp":1424917937000},"page":"593-600","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":22,"title":["Adipose Tissue\u2013Derived Regenerative Cell\u2013Enhanced Lipofilling for Treatment of Cryptoglandular Fistulae-in-Ano"],"prefix":"10.1177","volume":"22","author":[{"given":"David W.","family":"Borowski","sequence":"first","affiliation":[{"name":"North Tees and Hartlepool NHS Foundation Trust, University Hospital of North Tees, Stockton-on-Tees, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Talvinder 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