{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T23:59:22Z","timestamp":1785455962634,"version":"3.56.0"},"reference-count":36,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["OMICS: A Journal of Integrative Biology"],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:p>\n                    Ectonucleoside triphosphate diphosphohydrolase 2 (\n                    <jats:italic toggle=\"yes\">ENTPD2<\/jats:italic>\n                    ), an enzyme involved in extracellular nucleotide metabolism and purinergic signaling, has been linked to tumor\u2013immune interactions, although its role in colorectal cancer (CRC) remains unclear. This study examined the expression pattern and regulatory context of\n                    <jats:italic toggle=\"yes\">ENTPD2<\/jats:italic>\n                    through integrative analysis of transcriptomic, proteomic, microRNA (miRNA), and single-cell transcriptomic datasets. Transcriptomic analyses showed that\n                    <jats:italic toggle=\"yes\">ENTPD2<\/jats:italic>\n                    mRNA levels are elevated in colorectal tumors compared with normal tissues and that higher expression is associated with shorter relapse-free survival. In contrast, proteomic analyses indicated reduced\n                    <jats:italic toggle=\"yes\">ENTPD2<\/jats:italic>\n                    protein abundance in tumor samples, suggesting a divergence between transcript and protein expression. Analysis of candidate miRNAs identified miR-708-5p as a potential post-transcriptional regulator, supported by its increased expression in CRC and a predicted binding site within the\n                    <jats:italic toggle=\"yes\">ENTPD2<\/jats:italic>\n                    3\u2032-untranslated region (UTR). Single-cell transcriptomic datasets further indicated that\n                    <jats:italic toggle=\"yes\">ENTPD2<\/jats:italic>\n                    transcripts are mainly detected in malignant epithelial cells. We performed a functional validation using dual-luciferase reporter assays, qRT-PCR, and Western blot analysis in CRC cell lines. Experimental analyses demonstrated that miR-708-5p directly targets the\n                    <jats:italic toggle=\"yes\">ENTPD2<\/jats:italic>\n                    3\u2032UTR in HCT116 cells and suppresses\n                    <jats:italic toggle=\"yes\">ENTPD2<\/jats:italic>\n                    expression in both HCT116 and HT-29 cells. These findings support a potential contribution of miR-708-5p to\n                    <jats:italic toggle=\"yes\">ENTPD2<\/jats:italic>\n                    regulation in CRC.\n                  <\/jats:p>","DOI":"10.1177\/15578100261463392","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T10:38:45Z","timestamp":1782729525000},"page":"493-504","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>ENTPD2<\/i>\n                    Transcript-Protein Divergence in Colorectal Cancer and Its Association with miR-708-5p: An Integrative Analysis"],"prefix":"10.1177","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8923-2794","authenticated-orcid":false,"given":"Leyla","family":"Ata\u00e7 Do\u011fan","sequence":"first","affiliation":[{"name":"Department of Medical Biology, Tayfur S\u00f6kmen Faculty of Medicine, Hatay Mustafa Kemal University, Hatay, T\u00fcrkiye."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,6,29]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-023-10158-3"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.05005"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.4251\/wjgo.v18.i1.114502"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.947885"},{"issue":"3","key":"e_1_3_2_6_1","first-page":"1134","article-title":"Identification of prognostic immune-related signature predicting the overall survival for colorectal cancer","volume":"24","author":"Bai J","year":"2020","unstructured":"Bai J, , Zhang X, , Xiang ZX, et al. Identification of prognostic immune-related signature predicting the overall survival for colorectal cancer. Eur Rev Med Pharmacol Sci 2020;24(3):1134\u20131141; doi: 10.26355\/eurrev_202002_20164","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2024.102693"},{"key":"e_1_3_2_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/11795549251384582"},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neo.2017.05.002"},{"key":"e_1_3_2_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.01.035"},{"key":"e_1_3_2_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00530-7"},{"key":"e_1_3_2_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1262110"},{"key":"e_1_3_2_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.70041"},{"key":"e_1_3_2_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/b908315d"},{"key":"e_1_3_2_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/medsci11030044"},{"key":"e_1_3_2_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2016.206"},{"key":"e_1_3_2_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2011.57"},{"key":"e_1_3_2_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21031151"},{"key":"e_1_3_2_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/17590914221102068"},{"key":"e_1_3_2_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-024-03746-3"},{"key":"e_1_3_2_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21175998"},{"key":"e_1_3_2_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005535"},{"key":"e_1_3_2_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-S2-S3"},{"key":"e_1_3_2_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2009.07213.x"},{"key":"e_1_3_2_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac959"},{"key":"e_1_3_2_26_1","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2018.51.11.116"},{"key":"e_1_3_2_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1248"},{"key":"e_1_3_2_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2022.03.025"},{"key":"e_1_3_2_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-025-06143-9"},{"key":"e_1_3_2_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad1025"},{"key":"e_1_3_2_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.70215"},{"key":"e_1_3_2_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-024-01654-2"},{"issue":"9","key":"e_1_3_2_33_1","first-page":"5338","article-title":"miRNA-708 functions as a tumor suppressor in colorectal cancer by targeting ZEB1 through Akt\/mTOR signaling pathway","volume":"11","author":"Sun S","year":"2019","unstructured":"Sun S, , Hang T, , Zhang B, et al. miRNA-708 functions as a tumor suppressor in colorectal cancer by targeting ZEB1 through Akt\/mTOR signaling pathway. 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