{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T13:26:33Z","timestamp":1786973193128,"version":"build-2736575974"},"reference-count":40,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["OMICS: A Journal of Integrative Biology"],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:p>\n                    Fibromyalgia is a chronic pain syndrome characterized by widespread musculoskeletal pain, fatigue, sleep disturbances, and cognitive dysfunction, with substantial impact on quality of life and functional capacity. Despite its high prevalence, its underlying molecular mechanisms remain incompletely understood, and reliable biomarkers are lacking. This study performed an integrative analysis of publicly available transcriptomic datasets combined with protein\u2013protein interaction network analysis, hub gene identification, and investigation of microRNA (miRNA)-mediated posttranscriptional regulation, in addition to evaluating molecular modulation following therapeutic intervention. Differentially expressed genes consistently identified across independent cohorts revealed two major molecular axes: An inflammatory\u2013immune axis involving cytokine and interferon-related pathways, including\n                    <jats:italic toggle=\"yes\">IL6<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">TNF<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">CXCL8<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">STAT1<\/jats:italic>\n                    , and a structural axis associated with extracellular matrix organization, including\n                    <jats:italic toggle=\"yes\">COL1A1<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">COL3A1<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">FN1<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">ITGB1.<\/jats:italic>\n                    Integration with miRNA data demonstrated reduced expression of regulatory miRNAs linked to inflammatory and structural pathways, suggesting impaired posttranscriptional control. Therapeutic modulation analysis further demonstrated reduced expression of inflammatory genes and increased expression of structural genes following manual therapy, indicating partial reversibility of these molecular alterations. Collectively, these findings support the presence of multilevel molecular dysregulation in fibromyalgia and highlight potential biomarkers and therapeutic targets associated with inflammatory and peripheral structural mechanisms.\n                  <\/jats:p>","DOI":"10.1177\/15578100261468867","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T14:56:37Z","timestamp":1784213797000},"page":"585-593","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrative Analysis of Hub Genes and miRNAs Reveals Inflammatory and Structural Signatures in Fibromyalgia and Their Modulation Following Manual Therapy"],"prefix":"10.1177","volume":"30","author":[{"given":"Michele de Moura","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Universidade Federal da Grande Dourados (UFGD), Dourados, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariana Mendes","family":"Freire","sequence":"additional","affiliation":[{"name":"Universidade Federal da Grande Dourados (UFGD), Dourados, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana Mary Mestriner Felipe","family":"de Melo","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio da Grande Dourados (UNIGRAN), Dourados, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisele da Silva Peixoto","family":"Zandona","sequence":"additional","affiliation":[{"name":"Universidade Federal da Grande Dourados (UFGD), Dourados, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabete Castelon","family":"Konkiewitz","sequence":"additional","affiliation":[{"name":"Universidade Federal da Grande Dourados (UFGD), Dourados, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7821-1145","authenticated-orcid":false,"given":"Eduardo Henrique","family":"Loreti","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio da Grande Dourados (UNIGRAN), Dourados, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"key":"e_1_3_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2019.02.030"},{"key":"e_1_3_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21041186"},{"key":"e_1_3_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes14071312"},{"key":"e_1_3_8_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25179523"},{"key":"e_1_3_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26199762"},{"key":"e_1_3_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0239286"},{"key":"e_1_3_8_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0121903"},{"key":"e_1_3_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/dth.13191"},{"key":"e_1_3_8_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/1744806918819944"},{"key":"e_1_3_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9061814"},{"key":"e_1_3_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2022.06.006"},{"key":"e_1_3_8_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000003499"},{"key":"e_1_3_8_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241310443"},{"key":"e_1_3_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2024.12.149"},{"key":"e_1_3_8_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/brainsci15020206"},{"key":"e_1_3_8_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.874490"},{"key":"e_1_3_8_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells14040271"},{"key":"e_1_3_8_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2020-214902"},{"key":"e_1_3_8_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11092367"},{"key":"e_1_3_8_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001532"},{"issue":"2","key":"e_1_3_8_22_1","first-page":"S89","article-title":"Genome-wide expression profiling in the peripheral blood of patients with fibromyalgia","volume":"34","author":"Jones KD","year":"2016","unstructured":"Jones KD, , Gelbart T, , Whisenant TC, et al. Genome-wide expression profiling in the peripheral blood of patients with fibromyalgia. Clin Exp Rheumatol 2016;34(2 (Suppl 96)):S89\u2013S98.","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_3_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2008.01.004"},{"key":"e_1_3_8_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000002510"},{"key":"e_1_3_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-018-0672-2"},{"key":"e_1_3_8_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13018-025-05986-8"},{"key":"e_1_3_8_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells13050438"},{"key":"e_1_3_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurom.2022.11.007"},{"key":"e_1_3_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-53874-8"},{"key":"e_1_3_8_30_1","doi-asserted-by":"publisher","DOI":"10.2147\/JPR.S525610"},{"key":"e_1_3_8_31_1","doi-asserted-by":"publisher","DOI":"10.37349\/ei.2025.1003206"},{"key":"e_1_3_8_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keab146"},{"key":"e_1_3_8_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25115922"},{"key":"e_1_3_8_34_1","doi-asserted-by":"publisher","DOI":"10.55563\/clinexprheumatol\/iitbcp"},{"key":"e_1_3_8_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-015-0789-9"},{"key":"e_1_3_8_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22083891"},{"key":"e_1_3_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semarthrit.2024.152377"},{"key":"e_1_3_8_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25179551"},{"key":"e_1_3_8_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1275064"},{"key":"e_1_3_8_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-023-04477-w"},{"key":"e_1_3_8_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0198625"}],"container-title":["OMICS: A Journal of Integrative Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15578100261468867","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/15578100261468867","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15578100261468867","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T12:48:29Z","timestamp":1786970909000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/15578100261468867"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,16]]},"references-count":40,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10,1]]}},"alternative-id":["10.1177\/15578100261468867"],"URL":"https:\/\/doi.org\/10.1177\/15578100261468867","relation":{},"ISSN":["1536-2310","1557-8100"],"issn-type":[{"value":"1536-2310","type":"print"},{"value":"1557-8100","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,16]]}}}