{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T19:36:28Z","timestamp":1792870588195,"version":"4.5.0"},"reference-count":36,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T00:00:00Z","timestamp":1787356800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"The school Enterprise Cooperation Project","award":["DWSY202301"],"award-info":[{"award-number":["DWSY202301"]}]},{"name":"National Natural Science Foundation of China","award":["82001593"],"award-info":[{"award-number":["82001593"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["OMICS: A Journal of Integrative Biology"],"published-print":{"date-parts":[[2026,12,1]]},"abstract":"<jats:p>\n                    Nonsmall cell lung cancer (NSCLC) remains a major cause of cancer mortality, underscoring the need for multitarget therapeutic candidates with defined mechanisms. This study investigated the anti-NSCLC activity of Zishangan Granule (ZSG), a four-herb formulation, using serum metabolomics, network pharmacology, molecular docking, public multiomics datasets, and experimental validation. Ultra-performance liquid chromatography\u2013tandem mass spectrometry identified 15 serum-available constituents. Network analysis revealed 111 shared targets between these constituents and NSCLC-associated genes, with\n                    <jats:italic toggle=\"yes\">Tp53<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Casp3<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Bax<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Pten<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">Parp1<\/jats:italic>\n                    identified as core targets. Salvianolic acid L showed favorable predicted binding to these proteins, while public transcriptomic, proteomic, and immunohistochemical datasets supported their disease relevance. In a subcutaneous Lewis lung carcinoma (LLC) model, ZSG reduced tumor weight by 58.3% and increased tumor-cell apoptosis approximately threefold, accompanied by increased expression of\n                    <jats:italic toggle=\"yes\">P53<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">P21<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Bax<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Casp3<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Pten<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">Parp1<\/jats:italic>\n                    . ZSG also reduced viability and colony formation and induced apoptotic morphology in LLC and A549 cells. These findings support the anti-NSCLC activity of ZSG through\n                    <jats:italic toggle=\"yes\">P53<\/jats:italic>\n                    -associated\n                    <jats:italic toggle=\"yes\">Bax\/Casp3<\/jats:italic>\n                    apoptotic signaling and warrant further preclinical evaluation.\n                  <\/jats:p>","DOI":"10.1177\/15578100261480570","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T12:06:46Z","timestamp":1787400406000},"page":"758-769","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Serum Metabolomics and Multiomics Validation Reveal Zishangan Granule\u2013Induced\n                    <i>P53<\/i>\n                    -\n                    <i>Bax\/Casp3<\/i>\n                    Signaling in NSCLC"],"prefix":"10.1177","volume":"30","author":[{"given":"Muhammad","family":"Yousif","sequence":"first","affiliation":[{"name":"Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China."},{"name":"Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand, Pakistan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Liao","sequence":"additional","affiliation":[{"name":"Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoan","family":"Wang","sequence":"additional","affiliation":[{"name":"Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel Kumi","family":"Okyere","sequence":"additional","affiliation":[{"name":"Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongqiong","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Emergency\/Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2998-7879","authenticated-orcid":false,"given":"Yanchun","family":"Hu","sequence":"additional","affiliation":[{"name":"Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, College of Veterinary 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