{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:06:22Z","timestamp":1787061982779,"version":"build-2736575974"},"reference-count":38,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Suzhou Key Laboratory of Male Reproduction Research","award":["SZS201718"],"award-info":[{"award-number":["SZS201718"]}]},{"DOI":"10.13039\/100015798","name":"suzhou key medical center","doi-asserted-by":"publisher","award":["SZZX201505"],"award-info":[{"award-number":["SZZX201505"]}],"id":[{"id":"10.13039\/100015798","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Jiangsu Provincial Medical Innovation Team","award":["CXTDB2017013"],"award-info":[{"award-number":["CXTDB2017013"]}]},{"name":"Youth Science and Technology Project of \"Promoting Health through Science and Education\" in Suzhou","award":["KJXW2017063"],"award-info":[{"award-number":["KJXW2017063"]}]},{"name":"the Introduction Project of Suzhou Clinical Medicine Expert Team","award":["SZYJTD201708"],"award-info":[{"award-number":["SZYJTD201708"]}]},{"DOI":"10.13039\/501100001809","name":"national natural science foundation of china","doi-asserted-by":"publisher","award":["81401273"],"award-info":[{"award-number":["81401273"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Am J Mens Health"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:p>\n                    Reproductive tract inflammation is considered an important cause of male infertility. Increased leukocytes in semen can produce many reactive oxygen species (ROS), which affect sperm function. The aim of this study is to identify the main source of ROS in seminal plasma and to assess the effect of ROS on leukocytes. Semen samples ( n = 20) with leukocyte concentration &gt;1 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    were collected from a male infertility clinic. This study mainly compares the sperm function parameters of the normal group and the semen white blood cell group &gt;1 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    . The results identified that ROS in semen was closely related to sperm function parameters, and CD45\n                    <jats:sup>+<\/jats:sup>\n                    leucocytes were the main source of ROS. Compared with the control group, the concentration of IL-2, IL-4, IL-6, IFN-\u03b3, and TNF-\u03b1 was higher in the experimental group. Leukocytes in semen may regulate the secretion of ROS through the mammalian target of rapamycin (mTOR) pathway. A considerable amount of ROS can upregulate the expression of IL-6 in leukocytes via the nuclear factor kappa-B (NF-kB) pathway.\n                  <\/jats:p>","DOI":"10.1177\/1557988320970053","type":"journal-article","created":{"date-parts":[[2020,10,31]],"date-time":"2020-10-31T04:06:15Z","timestamp":1604117175000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":34,"title":["Reactive Oxygen Species Secreted by Leukocytes in Semen Induce Self-Expression of Interleukin-6 and Affect Sperm Quality"],"prefix":"10.1177","volume":"14","author":[{"given":"Xiaoping","family":"Li","sequence":"first","affiliation":[{"name":"Center for Reproduction and Genetics, The First Affiliated Hospital of Soochow University, SuZhou, Jiangsu, China"},{"name":"Biomedical Research Institute, Soochow University, SuZhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengxia","family":"Ni","sequence":"additional","affiliation":[{"name":"Center for Reproduction and Genetics, Nanjing Medical University Affiliated Suzhou Hospital, SuZhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyu","family":"Xing","sequence":"additional","affiliation":[{"name":"Center for Reproduction and Genetics, Nanjing Medical University Affiliated Suzhou Hospital, SuZhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Yu","sequence":"additional","affiliation":[{"name":"Center for Reproduction and Genetics, Nanjing Medical University Affiliated Suzhou Hospital, SuZhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Center for Reproduction and Genetics, Nanjing Medical University Affiliated Suzhou Hospital, SuZhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenmin","family":"Yang","sequence":"additional","affiliation":[{"name":"Center for Reproduction and Genetics, Nanjing Medical University Affiliated Suzhou Hospital, SuZhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Reproduction and Genetics, Nanjing Medical University Affiliated Suzhou Hospital, SuZhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhu","sequence":"additional","affiliation":[{"name":"Center for Reproduction and Genetics, Nanjing Medical University Affiliated Suzhou Hospital, SuZhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2456-4893","authenticated-orcid":false,"given":"Mutian","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Immunology, Anhui Medical University, HeFei, China"},{"name":"Center for Reproduction and Genetics, Nanjing Medical University Affiliated Suzhou Hospital, SuZhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,10,31]]},"reference":[{"issue":"126","key":"bibr1-1557988320970053","first-page":"1","volume":"12","author":"Agarwal A.","year":"2014","journal-title":"Reproductive Biology and Endocrinology"},{"key":"bibr2-1557988320970053","doi-asserted-by":"publisher","DOI":"10.5534\/wjmh.2014.32.1.1"},{"key":"bibr3-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a136237"},{"key":"bibr4-1557988320970053","doi-asserted-by":"publisher","DOI":"10.4103\/1008-682X.122203"},{"key":"bibr5-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)91139-L"},{"key":"bibr6-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12662"},{"key":"bibr7-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2009.49"},{"issue":"6","key":"bibr8-1557988320970053","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1002\/j.1939-4640.1996.tb01855.x","volume":"17","author":"Depuydt C. E.","year":"1996","journal-title":"Journal of Andrology"},{"key":"bibr9-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-014-0425-7"},{"key":"bibr10-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2006.08.103"},{"key":"bibr11-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2007.00852.x"},{"key":"bibr12-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2013.03.058"},{"key":"bibr13-1557988320970053","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.106.001149"},{"key":"bibr14-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2017.01.014"},{"key":"bibr15-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6793-11-10"},{"key":"bibr16-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)61893-7"},{"key":"bibr17-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2004.11.022"},{"key":"bibr18-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.2003.tb00863.x"},{"key":"bibr19-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-015-0454-x"},{"key":"bibr20-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/13.suppl_1.33"},{"key":"bibr21-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(00)00218-5"},{"key":"bibr22-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5172"},{"key":"bibr23-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.2005.00676.x"},{"key":"bibr24-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.02.010"},{"key":"bibr25-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2009.12.030"},{"key":"bibr26-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2009.01.004"},{"key":"bibr27-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12188"},{"key":"bibr28-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)46750-6"},{"key":"bibr29-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2008.00911.x"},{"issue":"3","key":"bibr30-1557988320970053","first-page":"106","volume":"188","author":"Muller V.","year":"1991","journal-title":"Archiv Fur Kriminologie"},{"key":"bibr31-1557988320970053","doi-asserted-by":"crossref","unstructured":"Nakajima S., Kitamura M. (2013). Bidirectional regulation of NF-\u03baB by reactive oxygen species: A role of unfolded protein response. Free Radical Biology & Medicine, 65(1), 162\u2013174.","DOI":"10.1016\/j.freeradbiomed.2013.06.020"},{"key":"bibr32-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-007-9071-5"},{"issue":"1","key":"bibr33-1557988320970053","first-page":"3155","volume":"9","author":"Sohrabi Y.","year":"2018","journal-title":"Frontiers in Immunology"},{"key":"bibr34-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)56412-7"},{"key":"bibr35-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)57588-8"},{"key":"bibr36-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2007.64"},{"issue":"1","key":"bibr37-1557988320970053","first-page":"1756494","volume":"2018","author":"Zhang M.","year":"2018","journal-title":"Mediators of Inflammation"},{"key":"bibr38-1557988320970053","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2017.01.009"}],"container-title":["American Journal of Men's Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1557988320970053","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1557988320970053","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1557988320970053","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T23:32:32Z","timestamp":1777505552000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1557988320970053"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9]]},"references-count":38,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1177\/1557988320970053"],"URL":"https:\/\/doi.org\/10.1177\/1557988320970053","relation":{},"ISSN":["1557-9883","1557-9891"],"issn-type":[{"value":"1557-9883","type":"print"},{"value":"1557-9891","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9]]},"article-number":"1557988320970053"}}