{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T23:58:08Z","timestamp":1790553488420,"version":"4.1.0"},"reference-count":52,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Dose-Response"],"published-print":{"date-parts":[[2020,1,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>To explore the role of microRNA (miR-21) in new bone formation in ankylosing spondylitis (AS) as mediated by different concentration of tumor necrosis factor-\u03b1 (TNF-\u03b1).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Fibroblasts isolated from the hips of patients with AS were induced to osteogenesis. These cells were then stimulated with varying concentrations of TNF-\u03b1. MicroRNA-21 expressions were evaluated using reverse transcription\u2013polymerase chain reaction (RT-PCR) and osteogenesis was detected via Alizarin Red S (ARS) staining and measurement of alkaline phosphatase (ALP) activity. Relative expressions of p-STAT3, Nuclear STAT3, cytoplasm STAT3, Runx2, BMP2, osteopontin, osteocalcin, and LC3B in AS fibroblasts were measured after exposure to different concentrations of TNF-\u03b1. The STAT3-inhibiting small interfering RNA allowed further exploration on its impact on miR-21 and primary miR-21 expressions. A proteoglycan-induced arthritis (PGIA) Balb\/c mouse model was established in order to monitor sacroiliac joint (SIJ) inflammation and subsequent damage through magnetic resonance image. Serum miR-21 and TNF-\u03b1 expressions were evaluated using RT-PCR and enzyme-linked immunosorbent assay. At week 16, mice models were transfected intravenously with miR-21 overexpressing agomir and miR-21 inhibiting antagomir for 7 successive days. The rate of abnormal bone formation at SIJ was evaluated using microcomputed tomography and hematoxylin and eosin staining at week 24. Western blot analysis enabled quantification of STAT-3, JAK-2, and interleukin (IL)-17A expressions present in the SIJ.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The in vitro miR-21 expression and osteogenesis activity were noted to be augmented in the setting of low TNF-\u03b1 concentrations (0.01-0.1 ng\/mL) while they were depressed in settings with higher TNF-\u03b1 concentrations (1-10 ng\/mL). Samples with the most distinct ARS manifestation and ALP activity as well as the highest miR-21 expressions were those who received 0.1 ng\/mL of TNF-\u03b1. Primary miR-21 was found to be notable raised by Si-STAT3, while the converse effect was seen in mature miR-21 expressions. Intravenous injection of exogenous miR-21 contributed to new bone formation and significantly elevated expressions of STAT3, JAK2, and IL-17 in PGIA mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>The results revealed that miR-21 may act as a potential mediator between new bone formation and inflammation in AS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1559325819901239","type":"journal-article","created":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T10:11:13Z","timestamp":1579601473000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":29,"title":["miR-21 may Act as a Potential Mediator Between Inflammation and Abnormal Bone Formation in Ankylosing Spondylitis Based on TNF-\u03b1 Concentration-Dependent Manner Through the JAK2\/STAT3 Pathway"],"prefix":"10.1177","volume":"18","author":[{"given":"Yu-Cong","family":"Zou","sequence":"first","affiliation":[{"name":"Department Of Rehabilitation Medicine, The Third Affiliated Hospital, Southern Medical University, Guang Zhou, Guangdong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Man","family":"Yan","sequence":"additional","affiliation":[{"name":"Guangzhou University of Chinese Medicine, Guang Zhou, Guangdong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Ping","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of TCM Orthopedics & Traumatology, The Third Affiliated Hospital, Southern Medical University, Guang Zhou, Guangdong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi- Yun","family":"Wang","sequence":"additional","affiliation":[{"name":"ShunDe Hospital, Southern Medical University, FoShan, Guangdong Province, 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