{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T19:08:00Z","timestamp":1787944080013,"version":"build-2784847793"},"reference-count":59,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Dose-Response"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>Background: Phytomedicine is becoming more acceptable as an alternative medicinal approach in the modern era.<\/jats:p>\n                  <jats:p>Objectives: The current study examined the antioxidant capacity and in vitro response of phytochemical constituents of\u00a0 Withania somnifera\u00a0(Ashwagandha) on standard parameters of healthy volunteer semen.<\/jats:p>\n                  <jats:p>Methods: The phytochemicals\u00a0and their pharmacological response in a hydroethanolic (30:70 v\/v) extract of\u00a0 W.\u00a0 somnifera\u00a0roots were determined using standard protocols.<\/jats:p>\n                  <jats:p>\n                    Results: The constituents included flavonoids, phenolic acids, alkaloids, and terpenoids were reported. High-performance liquid chromatography and Fourier-transform infrared spectroscopy determined a diverse array of biologically active chemical constituents in the extract. The extract of\u00a0 W. somnifera\u00a0exhibits substantial antioxidant properties, including total antioxidant capacity, 2,2-diphenyl-1-picrylhydrazyl inhibition, H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u00a0scavenging, and Fe\n                    <jats:sup>3+<\/jats:sup>\n                    \u00a0reducing potential ( P &lt; .05). The analysis of essential natural minerals explored adequate levels determined using atomic absorption spectrophotometer. Cytotoxic studies revealed significant thrombolytic, RBC membrane stabilization, and DNA damage protection activity ( P &lt; .05) while remaining non-mutagenic against\u00a0 Salmonella typhi\u00a0TA98 and TA100. The best protective response of\u00a0 W. somnifera\u00a0extract on human semen parameters (n = 30), such as total motility, progressive motility, and viability, demonstrated a significant ( P\u00a0&lt; .05) improvement, particularly at the dose of 25\u00a0\u03bcg\/mL and 50\u00a0\u03bcg\/mL.\n                  <\/jats:p>\n                  <jats:p>Conclusion: The study concludes that\u00a0 W. somnifera\u00a0possesses favorable in vitro characteristics that could aid in the preservation of sperm during intrauterine insemination and in vitro fertilization.<\/jats:p>","DOI":"10.1177\/15593258221074936","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T11:46:13Z","timestamp":1646135173000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":32,"title":["<i>Withania somnifera<\/i>\n                    Chemical Constituents\u2019 In Vitro Antioxidant Potential and Their Response on Spermatozoa Parameters"],"prefix":"10.1177","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0380-1332","authenticated-orcid":false,"given":"Naveed","family":"Munir","sequence":"first","affiliation":[{"name":"Department of Basic Medical Sciences, School of Health Sciences, University of Management and Technology, Lahore, Pakistan"},{"name":"Department of Biochemistry, Government College University Faisalabad, Faisalabad, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahed","family":"Mahmood","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Government College University Faisalabad, Faisalabad, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Shahid","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Agriculture Faisalabad, Faisalabad, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Naveed","family":"Afzal","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, School of Health Sciences, University of Management and Technology, Lahore, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Jahangir","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Government College University Faisalabad, Faisalabad, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed Muhammad","family":"Ali Shah","sequence":"additional","affiliation":[{"name":"Department of Eastern Medicine and Surgery, Government College University Faisalabad, Faisalabad, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imtiaz Mahmood","family":"Tahir","sequence":"additional","affiliation":[{"name":"College of Allied Health Professionals, Government College University Faisalabad, Faisalabad, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5524-7735","authenticated-orcid":false,"given":"Muhammad","family":"Riaz","sequence":"additional","affiliation":[{"name":"Department of Allied Health Sciences, Sargodha Medical College, University of Sargodha, Sargodha, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoukat","family":"Hussain","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Government College University Faisalabad, Faisalabad, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7457-8572","authenticated-orcid":false,"given":"Muhammad","family":"Akram","sequence":"additional","affiliation":[{"name":"Department of Eastern Medicine and Surgery, Government College University Faisalabad, Faisalabad, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima","family":"Yousaf","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Agriculture Faisalabad, Faisalabad, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,3,1]]},"reference":[{"key":"bibr1-15593258221074936","unstructured":"World Health Organization. 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