{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:14:36Z","timestamp":1787019276805,"version":"build-2736575974"},"reference-count":48,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Dose-Response"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Nitric oxide (NO) as a vaso- and cardio-protective agent could reduce vasomotor dysfunction in different cardiovascular diseases. One of the current therapeutics targeted at NO availability in the vascular wall are highly diluted antibodies to endothelial NO-synthase (eNOS). This drug has previously shown its endothelium-protective effect and effectiveness in reducing hypertension. Current study was dedicated to evaluate the direct impact of highly diluted antibodies to eNOS on the vessel constriction and dilation ex vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>For that purpose, we used thoracic aortas dissected from spontaneously hypertensive (SHR) rats. Endothelium-dependent relaxation in the presence of highly diluted antibodies to eNOS (1\u00a0mL) was examined after phenylephrine-induced pre-constriction of the aorta rings in response to gradually increased acetylcholine concentration (1\u00a0nM to 10\u00a0\u00b5M).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Highly diluted antibodies to eNOS enhanced acetylcholine-induced relaxation in a statistically significant manner. Moreover, it was demonstrated that observed effect was similar to perindopril, a well-known angiotensin-converting-enzyme inhibitor, which works through relaxing and widening blood vessels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings indicate that highly diluted antibodies to eNOS restored impaired endothelium function, as demonstrated by increased relaxation of SHR rats aorta rings. The revealed results suggest beneficial effect of highly diluted antibodies to eNOS to ameliorate hypertension and related diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/15593258221099281","type":"journal-article","created":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T19:56:56Z","timestamp":1652903816000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Highly Diluted Antibodies to eNOS Restore Endothelium Function in Aortic Rings From Hypertensive Rats"],"prefix":"10.1177","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2192-7302","authenticated-orcid":false,"given":"Nataliya V.","family":"Petrova","sequence":"first","affiliation":[{"name":"FSBSI Institute of General Pathology and Pathophysiology, Moscow, Russia"},{"name":"OOO \u201cNPF \u201cMATERIA MEDICA HOLDING\u201d, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey A.","family":"Tarasov","sequence":"additional","affiliation":[{"name":"FSBSI Institute of General Pathology and Pathophysiology, Moscow, Russia"},{"name":"OOO \u201cNPF \u201cMATERIA MEDICA HOLDING\u201d, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleg I.","family":"Epstein","sequence":"additional","affiliation":[{"name":"FSBSI Institute of General Pathology and Pathophysiology, Moscow, Russia"},{"name":"OOO \u201cNPF \u201cMATERIA MEDICA HOLDING\u201d, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Dubroca","sequence":"additional","affiliation":[{"name":"Cardiomedex, Escalquens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Sulpice","sequence":"additional","affiliation":[{"name":"Cardiomedex, Escalquens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,5,17]]},"reference":[{"key":"bibr1-15593258221099281","doi-asserted-by":"publisher","DOI":"10.2174\/1874192401004010302"},{"key":"bibr2-15593258221099281","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199322004-00002"},{"key":"bibr3-15593258221099281","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-200111002-00004"},{"key":"bibr4-15593258221099281","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2006.08.002"},{"key":"bibr5-15593258221099281","doi-asserted-by":"publisher","DOI":"10.4149\/gpb_2010_04_319"},{"key":"bibr6-15593258221099281","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13517"},{"key":"bibr7-15593258221099281","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706495"},{"key":"bibr8-15593258221099281","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2038"},{"key":"bibr9-15593258221099281","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.111559"},{"key":"bibr10-15593258221099281","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/290805"},{"key":"bibr11-15593258221099281","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01308.x"},{"key":"bibr12-15593258221099281","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvr233"},{"key":"bibr13-15593258221099281","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2015.02.020"},{"key":"bibr14-15593258221099281","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2018.10.001"},{"key":"bibr15-15593258221099281","doi-asserted-by":"publisher","DOI":"10.1002\/art.21891"},{"key":"bibr16-15593258221099281","unstructured":"Zavbert EV. Experimental study of pharmacological activity of antibodies to endothelial NO-Synthase in the released\u2013active form on the model of erectile dysfunction. PhD Thesis. Tomsk: Federal State Budgetary Institution \u201cScientific Research Institute of Pharmacology and Regenerative Medicine named after E.D. Goldberg\u201d. 2016. (In Russ.)"},{"key":"bibr17-15593258221099281","doi-asserted-by":"publisher","DOI":"10.1007\/s10517-010-0751-4"},{"issue":"4","key":"bibr18-15593258221099281","first-page":"116","volume":"99","author":"Belous AS","year":"2011","journal-title":"Scientific Bulletins of BelSU. Series: Medicine. 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