{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T12:55:56Z","timestamp":1777553756905,"version":"3.51.4"},"reference-count":37,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Dose-Response"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Pinus roxburghii<\/jats:italic>\n                      Sarg. (Pinaceae) has shown useful effects in the treatment of urinary problems. This research aimed to explore\n                      <jats:italic>P.roxburghii<\/jats:italic>\n                      scientifically via in-vitro, in-vivo and in-silico techniques to find out its therapeutic benefits on kidney and bladder.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>\n                      Pharmacologically active components of aqueous ethanolic extract of\n                      <jats:italic>P.roxburghii<\/jats:italic>\n                      were analyzed by HPLC-DAD screening. These components were predicted for multfi-mechanisms and spasmolytic effects in network pharmacology. In-vitro experiments were conducted on isolated bladder of rat to analyze the spasmolytic effects of plant extract. Spasmolytic effects were also compared with the standard drugs verapamil and oxybutynin In-vivo experiments were also performed to observe diuretic and nephroprotective potential of the plant.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result<\/jats:title>\n                    <jats:p>\n                      Phytochemical analysis indicated the presence of chlorogenic acid, gallic acid, P-coumaric acid, kaempferol, salicylic acid, sinapic acid and benzoic acid. Aqueous ethanolic extract of plant relaxed the contractions induced by high K\n                      <jats:sup>+<\/jats:sup>\n                      (80\u00a0mm) and carbachol (1\u00a0\u00b5M) in isolated urinary bladder strips of rat. In-vivo experiments revealed the nephroprotective and diuretic potential.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>So it has been proved via scientific evidence that the concerned plant possesses spasmolytic, nephroprotective and diuretic potential and may be an important therapeutic agent against urinary problems in the near future.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/15593258251352705","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T04:18:24Z","timestamp":1757391504000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Unveiling Nephroprotective, Diuretic and Toxicity Response of\n                    <i>Pinus roxburghii<\/i>\n                    Sarg. Extract by\n                    <i>In-vitro, In-vivo<\/i>\n                    and Docking Techniques"],"prefix":"10.1177","volume":"23","author":[{"given":"Maddiha","family":"Nasir","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6242-7147","authenticated-orcid":false,"given":"Fatima","family":"Saqib","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Farhaj","family":"Latif","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Codrut loan","family":"Ciurea","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Transilvania University of Brasov, Multan, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catalin","family":"Misarca","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Transilvania University of Brasov, Multan, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marius Alexandru","family":"Moga","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Transilvania University of Brasov, Multan, Pakistan"},{"name":"Academy of Medical Sciences of Romania, Brasov, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oana","family":"Andreescu","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Transilvania University of Brasov, Multan, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,9,8]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-016-1011-6"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/5938610"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/903246"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-010-9424-0"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.17179\/excli2016-670"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani13081388"},{"key":"e_1_3_5_8_2","volume-title":"Wood Oilagainst Carbon Tetrachloride-And Ethanol-Induced Hepatotoxicity","author":"Khan I","year":"2012","unstructured":"Khan I, Chaudhary VSand AK. 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