{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T04:08:07Z","timestamp":1793419687213,"version":"4.5.0"},"reference-count":41,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"The Second Affiliated Hospital of Chengdu Medical College, Nuclear Industry 416 Hospital","award":["2024ZX01"],"award-info":[{"award-number":["2024ZX01"]}]},{"name":"Distinguished Medical Expert in Jiangsu Province","award":["JSTPYXZJ2021006"],"award-info":[{"award-number":["JSTPYXZJ2021006"]}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20201183"],"award-info":[{"award-number":["BK20201183"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFC2503700, 2022YFC2503703"],"award-info":[{"award-number":["2022YFC2503700, 2022YFC2503703"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Postgraduate Research & Practice Innovation Program of Jiangsu Province","award":["KYCX23_3262"],"award-info":[{"award-number":["KYCX23_3262"]}]},{"name":"National Health Commission (NHC) Key Laboratory of Nuclear Technology Medical Transformation","award":["2023HYX005"],"award-info":[{"award-number":["2023HYX005"]}]},{"name":"Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institution"},{"name":"Wuxi Science and Technology Bureau, \u201cTaihu Light\u201d Science and Technology Research program","award":["K20241002"],"award-info":[{"award-number":["K20241002"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073476, 82404192, 82473565, and U24A20765"],"award-info":[{"award-number":["82073476, 82404192, 82473565, and U24A20765"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Innovative and Entrepreneurial Talent in Jiangsu Province","award":["JSSCRC2021568"],"award-info":[{"award-number":["JSSCRC2021568"]}]},{"name":"the Scientific Research Project of Wuxi Health Commission","award":["Q202433"],"award-info":[{"award-number":["Q202433"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Dose-Response"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To elucidate the role of NEDD4 in ionizing radiation (IR)-induced endothelial-mesenchymal transition (EndMT) and its molecular mechanism in radiation-induced lung injury (RILI), given the unclear regulatory pathways of EndMT in RILI pathogenesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      IR-induced EndMT was observed during RILI\n                      <jats:italic toggle=\"yes\">in<\/jats:italic>\n                      <jats:italic toggle=\"yes\">vivo<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">in<\/jats:italic>\n                      <jats:italic toggle=\"yes\">vitro<\/jats:italic>\n                      by immunohistochemical staining and Western blot analysis. Proteomics identified NEDD4 as a candidate, validated by RNA sequencing (RNA-seq) and quantitative real-time polymerase chain reaction (qRT\u2012PCR). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis linked NEDD4 to PI3K-AKT signaling. Co-immunoprecipitation (Co-IP) confirmed NEDD4-ATM interaction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>IR upregulated NEDD4 in endothelial cells, correlating with EndMT progression. NEDD4 overexpression enhanced ATM pathway activation, modulating genes upstream\/downstream of ATM. Co-IP verified physical NEDD4-ATM binding, suggesting NEDD4 stabilizes ATM to promote EndMT.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Overall, our study shows that NEDD4 mediates EndMT to participate in RILI through the ATM signaling pathway, which may break new ground for understanding the occurrence and development of RILI.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/15593258251352726","type":"journal-article","created":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T19:53:51Z","timestamp":1750449231000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["NEDD4-Mediated Endothelial-Mesenchymal Transition Participates in Radiation-Induced Lung Injury Through the ATM Signaling Pathway"],"prefix":"10.1177","volume":"23","author":[{"given":"Yang","family":"Feng","sequence":"first","affiliation":[{"name":"Soochow University"},{"name":"Wuxi No. 2 People\u2019s Hospital, Jiangnan University Medical Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lirong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Soochow University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youbin","family":"Zhang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Soochow University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Xu","sequence":"additional","affiliation":[{"name":"Soochow University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaixiao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Soochow University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Yang","sequence":"additional","affiliation":[{"name":"Suzhou Science & Technology Town Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Soochow University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Soochow University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Cao","sequence":"additional","affiliation":[{"name":"Soochow University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Wang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Soochow University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5452-5553","authenticated-orcid":false,"given":"Yang","family":"Jiao","sequence":"additional","affiliation":[{"name":"Soochow University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,6,20]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.00877"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2019.03.033"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.21037\/tlcr-22-108"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2019.03.024"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2005.11.006"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.radonc.2011.06.017"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2016.6748"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10060910"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-022-00887-5"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2005189"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.15334"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26801"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19030727"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-61292-9"},{"key":"e_1_3_4_16_2","first-page":"fj201701099","article-title":"PKC\u03b4 inhibition as a novel medical countermeasure for radiation-induced vascular damage","author":"Soroush F","year":"2018","unstructured":"Soroush F, Tang Y, Zaidi HM, Sheffield JB, Kilpatrick LE, Kiani MF. PKC\u03b4 inhibition as a novel medical countermeasure for radiation-induced vascular damage. FASEB J. 2018;fj201701099. Online ahead of print.","journal-title":"FASEB J"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1080\/09553002.2020.1683643"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/10715762.2016.1189078"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/1748-717X-9-111"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1080\/09553000601129093"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1667\/RR15384.1"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2017.04.020"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr070537c"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.613687"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1667\/RR2562.1"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.0901412"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/4135806"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21124271"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-14-3193"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/2211-5463.12552"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2013.03.024"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2021.09.033"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-4328-8"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2022.109999"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2017.1308617"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15743-6"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.48437"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.73.011303.073723"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2015.11.019"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells8101211"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-019-1161-8"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-03636-7"}],"container-title":["Dose-Response"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15593258251352726","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/15593258251352726","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/15593258251352726","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T18:02:57Z","timestamp":1777399377000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/15593258251352726"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":41,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1177\/15593258251352726"],"URL":"https:\/\/doi.org\/10.1177\/15593258251352726","relation":{},"ISSN":["1559-3258","1559-3258"],"issn-type":[{"value":"1559-3258","type":"print"},{"value":"1559-3258","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4]]},"article-number":"15593258251352726"}}