{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T01:09:24Z","timestamp":1777943364647,"version":"3.51.4"},"reference-count":39,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Orthop Surg (Hong Kong)"],"published-print":{"date-parts":[[2016,8]]},"abstract":"\n Purpose<\/jats:title>\n \n To determine whether articular chondrocytes derived from osteoarthritic knee joints could evoke alloreactive proliferation of peripheral blood mononuclear cells (PBMC) and inhibit mitogenic activity of polyclonally activated CD4\n +<\/jats:sup>\n major histocompatibility complex (MHC) class II\u2013restricted T cells in vitro.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Osteoarthritic cartilages of 17 patients aged 61 to 85 years were harvested during total knee arthroplasty. Chondrocytes were cultured for experiments. PBMCs, CD4\n +<\/jats:sup>\n T cells, CD8\n +<\/jats:sup>\n T cells, and CD14\n +<\/jats:sup>\n monocytes from healthy subjects were also used. To investigate the allogeneic response and immunosuppressive properties of chondrocytes, assays for one-way mixed lymphocyte reaction (MLR), apoptosis, activated CD4\n +<\/jats:sup>\n T-cell proliferation, and cytotoxic CD8\n +<\/jats:sup>\n T-cells were performed. Chondrocyte cell-surface antigens were examined using flow cytometry.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Chondrocytes failed to trigger an allogeneic PBMC reaction and did not induce apoptosis of allogeneic PBMCs in the MLR assay. Chondrocytes inhibited the proliferation of polyclonally activated CD4\n +<\/jats:sup>\n T cells via cell-cell contact and escaped the allogeneic cytotoxic reactivity of CD8\n +<\/jats:sup>\n T cells. Chondrocytes expressed MHC class I but not MHC class II molecules or B7-1\/-2-positive co-stimulatory molecules.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Chondrocytes from osteoarthritic knees in older patients exhibited similar immunomodulatory properties in vitro to those in juveniles or adults.<\/jats:p>\n <\/jats:sec>","DOI":"10.1177\/1602400222","type":"journal-article","created":{"date-parts":[[2017,1,21]],"date-time":"2017-01-21T12:28:36Z","timestamp":1485001716000},"page":"232-239","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Alloreactivity and Immunosuppressive Properties of Articular Chondrocytes from Osteoarthritic Cartilage"],"prefix":"10.1177","volume":"24","author":[{"given":"Satomi","family":"Abe","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery, Asahikawa Medical University, 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