{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T01:39:02Z","timestamp":1789954742057,"version":"4.0.1"},"reference-count":23,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2014,3,18]],"date-time":"2014-03-18T00:00:00Z","timestamp":1395100800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vascular"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>We aimed to examine the effects of sildenafil and n-acetylcystein on ischemia\/reperfusion injury in femoral artery endothelium and gastrocnemius muscle.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Basic methods<\/jats:title>\n                    <jats:p>32 rats of Sprague-Dawley breed were randomly divided into four groups (n\u2009=\u20098). Median laparotomy was performed, then a 120-minute ischemia was created by microvascular clamping of infrarenal aorta, followed by the release of clamping. In sildenafil group, 1\u2009mg\/kg of sildenafil infusion and in the n-acetylcystein group, 100\u2009mg\/kg of n-acetylcystein infusion was administered after release of clamps. Blood samples and tissue samples of femoral artery and gastrocnemius muscle were extracted for a histopathological evaluation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Principal findings<\/jats:title>\n                    <jats:p>Serum levels of malondialdehyde in ischemia\/reperfusion group (6.16\u2009\u00b1\u20090.79) were higher compared to the control group (4.69\u2009\u00b1\u20090.33), whereas a significant decrease was detected in sildenafil (5.17\u2009\u00b1\u20090.50) and n-acetylcystein (4.96\u2009\u00b1\u20090.49) groups. Femoral artery tissue sections of the control group, mean tumor necrosis factor alpha and hypoxy-induced factor-1 alpha immunoreactivity were found to be negative. In the ischemia\/reperfusion group, mean tumor necrosis factor \u03b1 immunoreactivity was intense and mean hypoxy-induced factor-1 alpha immunoreactivity was 51\u201375%. In the ischemia\/reperfusion\u2009+\u2009Sildenafil and ischemia\/reperfusion\u2009+\u2009NAS groups, mean tumor necrosis factor \u03b1 immunoreactivity was slight and mean hypoxy-induced factor-1 alpha immunoreactivity was 26\u201350%.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In conclusion, sildenafil and n-acetylcystein may reduce femoral artery endothelium and gastrocnemius muscle injury following lower extremity ischemia\/reperfusion.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1708538114527939","type":"journal-article","created":{"date-parts":[[2014,3,18]],"date-time":"2014-03-18T22:01:41Z","timestamp":1395180101000},"page":"21-30","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["The effects of sildenafil and n-acetylcysteine on ischemia and reperfusion injury in gastrocnemius muscle and femoral artery endothelium"],"prefix":"10.1177","volume":"23","author":[{"given":"Volkan","family":"Aksu","sequence":"first","affiliation":[{"name":"Cardiovascular Surgery Department, Trakya University, Edirne, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volkan","family":"Y\u00fcksel","sequence":"additional","affiliation":[{"name":"Cardiovascular Surgery Department, Trakya University, Edirne, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serchat","family":"Chousein","sequence":"additional","affiliation":[{"name":"Cardiovascular Surgery Department, Trakya University, Edirne, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebru","family":"Ta\u015ftekin","sequence":"additional","affiliation":[{"name":"Pathology Department, Trakya University, Edirne, 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