{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T06:27:35Z","timestamp":1777789655581,"version":"3.51.4"},"reference-count":15,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T00:00:00Z","timestamp":1222819200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Biol Markers"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:p>\n                    Gene silencing may occur in breast cancer samples from patients presenting with occult metastatic cells in the bone marrow and one mechanism regulating gene suppression is heterochromatin formation. We have studied whether members of the heterochromatin protein 1 family (HP1\n                    <jats:sup>Hs\u03b1<\/jats:sup>\n                    , HP1\n                    <jats:sup>Hs\u03b2<\/jats:sup>\n                    and HP1\n                    <jats:sup>Hs\u03b3<\/jats:sup>\n                    ), which take part in chromatin packaging and gene expression regulation, were differentially expressed in tumors from patients with and without occult metastatic cells in their bone marrow. Tumor samples and bone marrow aspirates were obtained from 37 breast cancer patients. Median age was 63 years and 68% of the patients presented with clinical stage I\/II disease. Presence of occult metastatic cells in bone marrow was detected through keratin-19 expression by nested RT-PCR in samples from 20 patients (54.1%). The presence of occult metastatic cells in bone marrow was not associated with node involvement, histological grade, estrogen receptor and ERBB2 immunoexpression. Relative gene expression of HP1\n                    <jats:sup>Hs\u03b1<\/jats:sup>\n                    , HP1\n                    <jats:sup>Hs\u03b2<\/jats:sup>\n                    and HP1\n                    <jats:sup>Hs\u03b3<\/jats:sup>\n                    was determined by real-time RT-PCR and did not vary according to the presence of occult metastatic cells in bone marrow. In addition, the combined expression of these three transcripts could not be used to classify samples according to the presence of bone marrow micrometastasis. Our work indicates that regulation of heterochromatin formation through HP1 family members may not be the sole mechanism implicated in the metastatic process to the bone marrow.\n                  <\/jats:p>","DOI":"10.1177\/172460080802300404","type":"journal-article","created":{"date-parts":[[2018,1,16]],"date-time":"2018-01-16T00:31:01Z","timestamp":1516062661000},"page":"219-224","source":"Crossref","is-referenced-by-count":1,"title":["Expression of Heterochromatin Protein 1 in the Primary Tumor of Breast Cancer Patients in the Presence or Absence of Occult Metastatic Cells in the Bone Marrow"],"prefix":"10.1177","volume":"23","author":[{"given":"A.P.S.","family":"Abreu","sequence":"first","affiliation":[{"name":"Faculdade de Medicina da Universidade de S\u00e3o Paulo, Departamento de Radiologia, Disciplina de Oncologia, S\u00e3o Paulo"},{"name":"Instituto Brasileiro de Controle do C\u00e2ncer de S\u00e3o Paulo, S\u00e3o Paulo - Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Milani","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina da Universidade de S\u00e3o Paulo, Departamento de Radiologia, Disciplina de Oncologia, S\u00e3o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.L.H.","family":"Katayama","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina da Universidade de S\u00e3o Paulo, Departamento de Radiologia, Disciplina de Oncologia, S\u00e3o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.M.","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Instituto Brasileiro de Controle do C\u00e2ncer de S\u00e3o Paulo, S\u00e3o Paulo - Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. Gomes","family":"da Fonseca","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina da Universidade de S\u00e3o Paulo, Departamento de Radiologia, Disciplina de Oncologia, S\u00e3o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.CS.","family":"G\u00f3es","sequence":"additional","affiliation":[{"name":"Instituto Brasileiro de Controle do C\u00e2ncer de S\u00e3o Paulo, S\u00e3o Paulo - Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.M.","family":"Brentani","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina da Universidade de S\u00e3o Paulo, Departamento de Radiologia, Disciplina de Oncologia, S\u00e3o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.A.A. Koike","family":"Folgueira","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina da Universidade de S\u00e3o Paulo, Departamento de Radiologia, Disciplina de Oncologia, S\u00e3o Paulo"},{"name":"Instituto Brasileiro de Controle do C\u00e2ncer de S\u00e3o Paulo, S\u00e3o Paulo - Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2018,1,15]]},"reference":[{"key":"bibr1-172460080802300404","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa050434"},{"key":"bibr2-172460080802300404","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.191367098"},{"key":"bibr3-172460080802300404","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa021967"},{"key":"bibr4-172460080802300404","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0701138104"},{"key":"bibr5-172460080802300404","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.03.8802"},{"key":"bibr6-172460080802300404","first-page":"5679","volume":"63","author":"Woelfle U.","year":"2003","journal-title":"Cancer Res"},{"key":"bibr7-172460080802300404","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2008.09.007"},{"key":"bibr8-172460080802300404","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-1284"},{"key":"bibr9-172460080802300404","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2008"},{"key":"bibr10-172460080802300404","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.080148"},{"key":"bibr11-172460080802300404","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2006000800013"},{"key":"bibr12-172460080802300404","first-page":"3359","volume":"60","author":"Kirschmann D.A.","year":"2000","journal-title":"Cancer Res"},{"key":"bibr13-172460080802300404","doi-asserted-by":"publisher","DOI":"10.1016\/j.molonc.2007.03.004"},{"key":"bibr14-172460080802300404","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6602189"},{"key":"bibr15-172460080802300404","doi-asserted-by":"publisher","DOI":"10.1023\/B:BREA.0000041583.72269.e1"}],"container-title":["The International Journal of Biological Markers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/172460080802300404","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/172460080802300404","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:57:36Z","timestamp":1777492656000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/172460080802300404"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,10]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2008,10]]}},"alternative-id":["10.1177\/172460080802300404"],"URL":"https:\/\/doi.org\/10.1177\/172460080802300404","relation":{},"ISSN":["0393-6155","1724-6008"],"issn-type":[{"value":"0393-6155","type":"print"},{"value":"1724-6008","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,10]]}}}