{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T06:38:48Z","timestamp":1777790328868,"version":"3.51.4"},"reference-count":16,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2020,1,29]],"date-time":"2020-01-29T00:00:00Z","timestamp":1580256000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["no.81572442 and no.81871293"],"award-info":[{"award-number":["no.81572442 and no.81871293"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003392","name":"natural science foundation of fujian province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Medical innovation in Fujian Province"},{"name":"Youth fund of Fujian Provincial Department of Health"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Biol Markers"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>\u03b1-fetoprotein (AFP) expression is activated during the embryonic stage or hepatocellular carcinogenesis, so it is presumed that AFP is a key endogenous molecule to promote cell proliferation or differentiation. We carried out gene screening in an unknown family with hyper-alpha-fetoproteinemia and some sporadic menopausal women, and discussed the relationship between AFP expression and liver cirrhosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Peripheral blood samples from family members, patients with malignant liver tumors, and normal controls were collected. Full-length sequence of AFP was amplified and directly sequenced, and compared with normal controls. HNF-1\u03b1 and HNF-1\u03b2 in plasma levels of family members, patients with liver cancer, newborns, pregnant women, and normal subjects were detected by ELISA, and the relationship between HNF-1 and AFP mutation or high expression was evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>There was a mutation in AFP promoter region at c.-200 C&gt;T, which was located at the binding site of AFP hepatocyte nuclear factor 1 (HNF-1). AFP was higher than 4000 ng\/L in all members carrying the mutation, but liver cancer was excluded in the family with hyper-alpha-fetoprotein. However, cirrhosis occurred in post-menopausal women. The cases reviewed showed that unknown hyper-alpha-fetoprotein was closely related to HNF-1 binding point of AFP in post-menopausal women with cirrhosis (7\/11), while the plasma levels of HNF-1\u03b1 and HNF-1\u03b2 were not significantly different.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The mutation of the HNF-1 binding point of AFP may lead to an abnormal high expression of AFP by altering the binding of HNF transcription factors, which is closely related to cirrhosis in menopausal women.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1724600819900510","type":"journal-article","created":{"date-parts":[[2020,1,29]],"date-time":"2020-01-29T04:37:12Z","timestamp":1580272632000},"page":"41-46","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["HNF-1 binding point mutation of the AFP gene promotes cirrhosis in post-menopausal women"],"prefix":"10.1177","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8720-1109","authenticated-orcid":false,"given":"Jing-wen","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathology, 1st Affiliated Hospital, Fujian Medical University, Fuzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Mindong Hospital, Fujian Medical University, Fuan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi-cai","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine Centre, 1st Affiliated Hospital, Fujian Medical University, Fuzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-zhong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hepatopancreatobiliary Surgery, 1st Affiliated Hospital, Fujian Medical University, Fuzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu-yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Medical Technology and Engineering College, Fujian Medical University, Fuzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-jia","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine Centre, 1st Affiliated Hospital, Fujian Medical University, Fuzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong-hong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine Centre, 1st Affiliated Hospital, Fujian Medical University, Fuzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing-ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine Centre, 1st Affiliated Hospital, Fujian Medical University, Fuzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine Centre, 1st Affiliated Hospital, Fujian Medical University, Fuzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pathology, 1st Affiliated Hospital, Fujian Medical University, Fuzhou, 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