{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T08:18:09Z","timestamp":1789805889236,"version":"4.0.1"},"reference-count":72,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Mol Pain"],"published-print":{"date-parts":[[2016,1,1]]},"abstract":"<jats:p>\n                    There are substantial disagreements about the expression of gastrin-releasing peptide (GRP) in sensory neurons and whether GRP antibody cross-reacts with substance P (SP). These concerns necessitate a critical revaluation of GRP expression using additional approaches. Here, we show that a widely used GRP antibody specifically recognizes GRP but not SP. In the spinal cord of mice lacking SP ( Tac1 KO), the expression of not only GRP but also other peptides, notably neuropeptide Y (NPY), is significantly diminished. We detected Grp mRNA in dorsal root ganglias using reverse transcription polymerase chain reaction, in situ hybridization and RNA-seq. We demonstrated that Grp mRNA and protein are upregulated in dorsal root ganglias, but not in the spinal cord, of mice with chronic itch. Few GRP\n                    <jats:sup>+<\/jats:sup>\n                    immunostaining signals were detected in spinal sections following dorsal rhizotomy and GRP\n                    <jats:sup>+<\/jats:sup>\n                    cell bodies were not detected in dissociated dorsal horn neurons. Ultrastructural analysis further shows that substantially more GRPergic fibers form synaptic contacts with gastrin releasing peptide receptor-positive (GRPR\n                    <jats:sup>+<\/jats:sup>\n                    ) neurons than SPergic fibers. Our comprehensive study demonstrates that a majority of GRPergic fibers are of primary afferent origin. A number of factors such as low copy number of Grp transcripts, small percentage of cells expressing Grp, and the use of an eGFP GENSAT transgenic as a surrogate for GRP protein have contributed to the controversy. Optimization of experimental procedures facilitates the specific detection of GRP expression in dorsal root ganglia neurons.\n                  <\/jats:p>","DOI":"10.1177\/1744806916643724","type":"journal-article","created":{"date-parts":[[2016,4,11]],"date-time":"2016-04-11T22:10:13Z","timestamp":1460412613000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":35,"title":["Critical evaluation of the expression of gastrin-releasing peptide in dorsal root ganglia and spinal cord"],"prefix":"10.1177","volume":"12","author":[{"given":"Devin M","family":"Barry","sequence":"first","affiliation":[{"name":"Center for the Study of Itch, Washington University School of Medicine, St. Louis, MO, USA"},{"name":"Departments of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[{"name":"Center for the Study of Itch, Washington University School of Medicine, St. Louis, MO, USA"},{"name":"Department of Anatomy, K. 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Leung Brain Research Centre, The Fourth Military Medical University, Xi\u2019an, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian-Yu","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for the Study of Itch, Washington University School of Medicine, St. Louis, MO, USA"},{"name":"Departments of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai-Feng","family":"Shen","sequence":"additional","affiliation":[{"name":"Center for the Study of Itch, Washington University School of Medicine, St. Louis, MO, USA"},{"name":"Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue-Ting","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for the Study of Itch, Washington University School of Medicine, St. Louis, MO, USA"},{"name":"Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, The State Key Laboratory of Respiratory Disease, The Second Affiliated Hospital, Guangzhou Medical University, Guangdong, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen-Yu","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Anatomy, K. 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Leung Brain Research Centre, The Fourth Military Medical University, Xi\u2019an, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Admire","family":"Munanairi","sequence":"additional","affiliation":[{"name":"Center for the Study of Itch, Washington University School of Medicine, St. Louis, MO, USA"},{"name":"Departments of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Jun","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Yin","sequence":"additional","affiliation":[{"name":"Center for the Study of Itch, Washington University School of Medicine, St. Louis, MO, USA"},{"name":"Departments of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Gang","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Qing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anatomy, K. 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Leung Brain Research Centre, The Fourth Military Medical University, Xi\u2019an, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou-Feng","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for the Study of Itch, Washington University School of Medicine, St. Louis, MO, USA"},{"name":"Departments of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA"},{"name":"Departments of Psychiatry, Center for the Study of Itch, Washington University School of Medicine, St. Louis, MO, USA"},{"name":"Departments of Developmental Biology, Center for the Study of Itch, Washington University School of Medicine, St. Louis, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2016,4,11]]},"reference":[{"key":"bibr1-1744806916643724","doi-asserted-by":"crossref","first-page":"2956","DOI":"10.1523\/JNEUROSCI.04-12-02956.1984","volume":"4","author":"O\u2019Donohue 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