{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T06:22:14Z","timestamp":1790490134514,"version":"4.1.0"},"reference-count":50,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Mol Pain"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Mycolactone is a polyketide toxin secreted by the mycobacterium Mycobacterium ulcerans, responsible for the extensive hypoalgesic skin lesions characteristic of patients with Buruli ulcer. A recent pre-clinical study proposed that mycolactone may produce analgesia via activation of the angiotensin II type 2 receptor (AT\n                      <jats:sub>2<\/jats:sub>\n                      R). In contrast, AT\n                      <jats:sub>2<\/jats:sub>\n                      R antagonist EMA401 has shown analgesic efficacy in animal models and clinical trials for neuropathic pain. We therefore investigated the morphological and functional effects of mycolactone in cultured human and rat dorsal root ganglia (DRG) neurons and the role of AT\n                      <jats:sub>2<\/jats:sub>\n                      R using EMA401. Primary sensory neurons were prepared from avulsed cervical human DRG and rat DRG; 24\u2009h after plating, neurons were incubated for 24 to 96\u2009h with synthetic mycolactone A\/B, followed by immunostaining with antibodies to PGP9.5, Gap43, \u03b2 tubulin, or Mitotracker dye staining. Acute functional effects were examined by measuring capsaicin responses with calcium imaging in DRG neuronal cultures treated with mycolactone.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Morphological effects: Mycolactone-treated cultures showed dramatically reduced numbers of surviving neurons and non-neuronal cells, reduced Gap43 and \u03b2 tubulin expression, degenerating neurites and reduced cell body diameter, compared with controls. Dose-related reduction of neurite length was observed in mycolactone-treated cultures. Mitochondria were distributed throughout the length of neurites and soma of control neurons, but clustered in the neurites and soma of mycolactone-treated neurons. Functional effects: Mycolactone-treated human and rat DRG neurons showed dose-related inhibition of capsaicin responses, which were reversed by calcineurin inhibitor cyclosporine and phosphodiesterase inhibitor 3-isobutyl-1-Methylxanthine, indicating involvement of cAMP\/ATP reduction. The morphological and functional effects of mycolactone were not altered by Angiotensin II or AT\n                      <jats:sub>2<\/jats:sub>\n                      R antagonist EMA401.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Mycolactone induces toxic effects in DRG neurons, leading to impaired nociceptor function, neurite degeneration, and cell death, resembling the cutaneous hypoalgesia and nerve damage in individuals with M. Ulcerans infection.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1744806916654144","type":"journal-article","created":{"date-parts":[[2016,6,20]],"date-time":"2016-06-20T21:53:49Z","timestamp":1466459629000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":27,"title":["Mycolactone-mediated neurite degeneration and functional effects in cultured human and rat DRG neurons"],"prefix":"10.1177","volume":"12","author":[{"given":"U","family":"Anand","sequence":"first","affiliation":[{"name":"Department of Medicine, Imperial College London, Hammersmith Hospital, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Sinisi","sequence":"additional","affiliation":[{"name":"Peripheral Nerve Injury Unit, Royal National Orthopaedic Hospital, Middlesex, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Fox","sequence":"additional","affiliation":[{"name":"Peripheral Nerve Injury Unit, Royal National Orthopaedic Hospital, Middlesex, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"MacQuillan","sequence":"additional","affiliation":[{"name":"Peripheral Nerve Injury Unit, Royal National Orthopaedic Hospital, Middlesex, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Quick","sequence":"additional","affiliation":[{"name":"Peripheral Nerve Injury Unit, Royal National Orthopaedic Hospital, Middlesex, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Korchev","sequence":"additional","affiliation":[{"name":"Department of Medicine, Imperial College London, Hammersmith Hospital, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Bountra","sequence":"additional","affiliation":[{"name":"University of Oxford Structural Genomics Consortium, Headington, Oxford, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"McCarthy","sequence":"additional","affiliation":[{"name":"Spinifex Pharmaceuticals Pty Ltd, St. Preston, VIC, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Anand","sequence":"additional","affiliation":[{"name":"Department of Medicine, Imperial College London, Hammersmith Hospital, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2016,6,20]]},"reference":[{"key":"bibr1-1744806916654144","unstructured":"WHO. 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