{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T22:02:40Z","timestamp":1793311360137,"version":"4.5.0"},"reference-count":51,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"the Construction Project of Anesthesiology Discipline Special Disease Center in Zhejiang North Region","award":["201524"],"award-info":[{"award-number":["201524"]}]},{"name":"Zhejiang provincial and Ministerial Cultivation Plan for Medicine and Health","award":["2015PYA010"],"award-info":[{"award-number":["2015PYA010"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81171057"],"award-info":[{"award-number":["81171057"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Jiaxing city science and technology project","award":["2017AY33008"],"award-info":[{"award-number":["2017AY33008"]}]},{"name":"Natural Science Foundation of Zhejiang Provincial","award":["LY16H090016"],"award-info":[{"award-number":["LY16H090016"]}]},{"name":"Natural Science Foundation of Zhejiang Provincial","award":["LY17H090019"],"award-info":[{"award-number":["LY17H090019"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81341035"],"award-info":[{"award-number":["81341035"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013115","name":"Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents","doi-asserted-by":"crossref","award":["2012-RC-22"],"award-info":[{"award-number":["2012-RC-22"]}],"id":[{"id":"10.13039\/501100013115","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Mol Pain"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Chemokine, monocyte chemoattractant protein-1 (MCP-1), is a potential factor to cause cancer-induced bone pain (CIBP). NF-\u03baB signaling is very important in mediating the expression of chemokines and may have a role in CIBP. However, the mechanism is still unclear. This study investigates the role of NF-\u03baB in CIBP by regulating MCP-1\/chemokine CC motif receptor-2 (CCR2) signaling pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>A rat CIBP model was established by injecting Walker-256 cells into the tibia medullary cavity. Nine days later, animals were intrathecally administrated with MCP-1 neutralizing antibody, CCR2 antagonist (RS504393), or NF-\u043aB inhibitor (BAY11\u20137081). Mechanical paw withdrawal threshold was used to assess pain behavior and sciatic functional index, and radiographic images were adopted to evaluate the damage of nerve and bone. The spinal cords were harvested for Western blot and quantitative reverse transcription polymerase chain reaction. The distribution of MCP-1, CCR2, and NF-\u043aB was detected by double immunofluorescent staining.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>CIBP caused remarkable bone destruction, injury of sciatic and femoral nerve, and persistent (&gt;15 days) mechanical allodynia in rats. Tumor cell inoculation upregulate MCP-1 and NF-\u043aB in activated neurons as well as CCR2 in neurons and microglia of the spinal cord. MCP-1 antibody, RS504393, and BAY11\u20137081 partially reversed CIBP-induced mechanical allodynia, and CIBP regulated the expression levels of pro-inflammatory cytokines, tumor necrosis factor-\u03b1 and interferon-\u03b3, and anti-inflammatory cytokine, interleukin 4, and BAY11\u20137081 lowered CIBP-induced MCP-1 and CCR2 expressions in a dose-dependent manner.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>In conclusion, NF-\u043aB signaling pathway regulates the expressions of MCP-1\/CCR2-induced inflammatory factors in the spinal cord of CIBP rats.<\/jats:p>\n                  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