{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T01:33:17Z","timestamp":1777944797904,"version":"3.51.4"},"reference-count":44,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science Grants","doi-asserted-by":"crossref","award":["17H04113"],"award-info":[{"award-number":["17H04113"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Hyogo College of Medicine Diversity Grant for Research Promotion","award":["Initiative for Realizing Diversity in the Research"],"award-info":[{"award-number":["Initiative for Realizing Diversity in the Research"]}]},{"name":"Grant-in-Aid for Researchers, Hyogo Medical University"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Mol Pain"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:p>Itch and pain are both unpleasant, but they are discrete sensations. Both of these sensations are transmitted by C-fibers and processed in laminae I-II of the dorsal horn. To examine whether pruriception modulates pain, we first confirmed the activation of cells in the itch-related circuits that were positive for gastrin-releasing peptide (GRP) and GRP receptor (GRPR) using a paw formalin injection model. This pain model with typical biphasic pain behavior increased c-Fos but did not affect the expressions of GRP and GRPR mRNAs in the dorsal horn. Using c-Fos expression as a marker for activated cells, we confirmed that formalin injection increased the number of cells double-labeled for c-Fos and GRP or GRPR in the dorsal horn. The emergence of these neurons indicates the activation of itch-related circuits by acute pain signals. The effect of an antagonist for a GRPR was examined in the paw formalin injection model. Intrathecal chronic antagonization of spinal GRPR enhanced the onset of phase II of paw formalin injection-induced pain behavior. Exogenous intrathecal GRP infusion to the paw-formalin injection model not only showed significant reduction of pain behavior but also increased c-Fos in the inhibitory neurons in the dorsal horn. The anti-nociceptive effect of spinal GRP infusion was observed in the peripheral inflammation model (complete Freund\u2019s adjuvant injection model). In this study we suggest that painful stimuli activated itch-related neuronal circuits and uncovered the spinal activation of the itch-induced analgesic effect on acute and established inflammatory pain.<\/jats:p>","DOI":"10.1177\/17448069221108965","type":"journal-article","created":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T04:14:03Z","timestamp":1657512843000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Analgesic effect of gastrin-releasing peptide in the dorsal horn"],"prefix":"10.1177","volume":"18","author":[{"given":"Ayano","family":"Saeki","sequence":"first","affiliation":[{"name":"Department of Anatomy and Neuroscience, Faculty of Medicine, Hyogo Medical University, Nishinomiya, Hyogo, Japan"},{"name":"Department of Anesthesiology and Pain Medicine, Faculty of Medicine, Hyogo Medical University, Nishinomiya, Hyogo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0604-0551","authenticated-orcid":false,"given":"Hiroki","family":"Yamanaka","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neuroscience, Faculty of Medicine, Hyogo Medical University, Nishinomiya, Hyogo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimiko","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neuroscience, Faculty of Medicine, Hyogo Medical University, Nishinomiya, Hyogo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8097-4304","authenticated-orcid":false,"given":"Masamichi","family":"Okubo","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neuroscience, Faculty of Medicine, Hyogo Medical University, Nishinomiya, Hyogo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Noguchi","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neuroscience, Faculty of Medicine, Hyogo Medical University, Nishinomiya, Hyogo, 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