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Herein, we assessed the analgesic effect of \u03b2-elemene, a chief component in the traditional Chinese medicine Curcuma wenyujin, and explored the underlying mechanisms at the level of spinal dorsal horn (SDH) under neuropathic pain. A spared nerve injury (SNI)-induced neuropathic pain model was established in rats. Intraperitoneal injection (i.p.) of \u03b2-elemene was administered for 21 consecutive days. Mechanical allodynia was explored by von Frey filaments. The activation of the mitogen-activated protein kinase (MAPK) family (including ERK, p38, and JNK) in spinal neurons, astrocytes, and microglia was evaluated using immunostaining 29 days after SNI surgery. The expression of GFAP, Iba-1, p-ERK, p-JNK, and p-p38 within the SDH was measured using immunoblotting. The levels of proinflammatory cytokines (including TNF-\u03b1, IL-1\u03b2, and IL-6) were measured with ELISA. The levels of oxidative stress indicators (including MDA, SOD, and GSH-PX) were detected using biochemical tests. Consecutive i.p. administration of \u03b2-elemene relieved SNI-induced mechanical allodynia (with an EC50 of 16.40 mg\/kg). SNI significantly increased the expression of p-ERK in spinal astrocytes but not microglia on day 29. \u03b2-elemene reversed spinal astrocytic ERK activation and subsequent upregulation of proinflammatory cytokines in SNI rats, with no effect on the expression of p38 and JNK in spinal glia. \u03b2-elemene also exerted antioxidative effects by increasing the levels of SOD and GSH-PX and decreasing the level of MDA. Our results suggest that SNI induces robust astrocytic ERK activation within the SDH in the late phase of neuropathic pain. \u03b2-elemene exerts remarkable analgesic effects on neuropathic pain, possibly by inhibiting spinal astrocytic ERK activation and subsequent neuroinflammatory processes. Our findings suggest that \u03b2-elemene might be a promising analgesic for the treatment of chronic pain.<\/jats:p>","DOI":"10.1177\/17448069221121562","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T13:36:14Z","timestamp":1660743374000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":18,"title":["The analgesic effects of \u03b2-elemene in rats with neuropathic pain by inhibition of spinal astrocytic ERK activation"],"prefix":"10.1177","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7177-4439","authenticated-orcid":false,"given":"Li-Tian","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Traditional Chinese Medicine, Tangdu Hospital, Air Force Medical University, Xi\u2019an, China"},{"name":"Department of Gastroenterology, Tangdu Hospital, Air Force Medical University, Xi'an, 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, Preclinical School of Medicine, Air Force Medical University, Xi\u2019an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo-Yuan","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The Second Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an Jiaotong University, Xi\u2019an, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Qiao","sequence":"additional","affiliation":[{"name":"Laser Medical Center, Hainan Hospital, PLA General Hospital, Sanya, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Yu","family":"Yan","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Dalian Medical University, Dalian, 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