{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T01:34:23Z","timestamp":1777944863317,"version":"3.51.4"},"reference-count":37,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Natural Science Foundation of Shandong Province of China","award":["No. ZR2021MH023"],"award-info":[{"award-number":["No. ZR2021MH023"]}]},{"name":"the Jinan University 20 Articles Research Leader Studio Project","award":["No. 2021GXRC098"],"award-info":[{"award-number":["No. 2021GXRC098"]}]},{"name":"the Key Research and Development Program of Shandong Province","award":["No. 2022ZLGX03"],"award-info":[{"award-number":["No. 2022ZLGX03"]}]},{"name":"the Scientific Research Innovation Team Project of the Affiliated Hospital of Shandong University of Traditional Chinese Medicine","award":["No. 2024-72"],"award-info":[{"award-number":["No. 2024-72"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Mol Pain"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>\n                      To investigate the analgesic effects and mechanisms of BMSCs-IL-10\n                      <jats:sup>+<\/jats:sup>\n                      administered via intrathecal injection in CCD rats.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients and methods:<\/jats:title>\n                    <jats:p>\n                      After CCD surgery, rats were administered intrathecal injections of PBS, BMSCs, BMSCs-IL-10\n                      <jats:sup>+<\/jats:sup>\n                      , BMSCs-IL-10\n                      <jats:sup>+<\/jats:sup>\n                      +Anti-IL-10, LV-IRF-8, and LV-IRF-8+IL-10. Pain was assessed by measuring the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL). Glial cell activation and M2 microglial polarization were evaluated by immunofluorescence staining of Iba-1, GFAP, and Arg-1, and by WB for Iba-1 and Arg-1. Spinal cord inflammation was assessed by PCR analysis of TGF-\u03b2, TNF-\u03b1, and IL-1\u03b2 expression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Compared with the CCD+PBS group, intrathecal injection of both BMSCs and BMSCs-IL-10\n                      <jats:sup>+<\/jats:sup>\n                      significantly alleviated CCD-induced mechanical and thermal pain. However, the analgesic effect of the BMSCs group markedly decreased after 4 days, while the BMSCs-IL-10\n                      <jats:sup>+<\/jats:sup>\n                      group lasted at least 14 days. The BMSCs-IL-10\n                      <jats:sup>+<\/jats:sup>\n                      group significantly upregulated the expression of TGF-\u03b2 while downregulating TNF-\u03b1 and IL-1\u03b2, inhibiting glial cell activation and promoting M2 microglia polarization. These effects were superior to the BMSCs group and could be abolished by anti-IL-10 antibody. IRF-8 overexpression exacerbated pain and inflammation in CCD rats, but the combined application of IL-10 protein reversed this impact.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      IL-10 is a key cytokine mediating the analgesic effects of BMSCs. Transplantation of BMSCs-IL-10\n                      <jats:sup>+<\/jats:sup>\n                      cells reduces glial activation, alleviates neuroinflammation, and relieves neuropathic pain by enhancing IL-10 expression and suppressing IRF-8.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/17448069261432034","type":"journal-article","created":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T12:32:11Z","timestamp":1772195531000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Study on the role and mechanism of the IL-10-IRF-8 signaling pathway in bone marrow mesenchymal stem cell therapy for pain in CCD rats"],"prefix":"10.1177","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-9576-9125","authenticated-orcid":false,"given":"Yican","family":"Yang","sequence":"first","affiliation":[{"name":"Rehabilitation and Physical Therapy Department, Shandong University of Traditional Chinese Medicine Affiliated Hospital, Jinan, China"},{"name":"Rehabilitation Center, Qilu Hospital of Shandong University, Jinan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mutong","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Nursing and Rehabilitation, Shandong University, Jinan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Guo","sequence":"additional","affiliation":[{"name":"Rehabilitation Center, Qilu Hospital of Shandong University, Jinan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, Weihai Wendeng District Integrated Traditional Chinese and Western Medicine Hospital, Weihai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Rehabilitation and Physical Therapy Department, Shandong University of Traditional Chinese Medicine Affiliated Hospital, Jinan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shouwei","family":"Yue","sequence":"additional","affiliation":[{"name":"Rehabilitation Center, Qilu Hospital of Shandong University, Jinan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2026,2,27]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lpm.2024.104232"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.hcl.2015.12.011"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/medicina61061063"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01014"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering10050621"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3344\/kjp.24374"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-019-1631-0"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000001921"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2023.04.002"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep42260"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells11172630"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1515\/tnsci-2020-0139"},{"issue":"4","key":"e_1_3_3_14_2","article-title":"Pulsed radiofrequency on dorsal root ganglion relieved neuropathic pain associated with downregulation of the spinal interferon regulatory factor 8, microglia, p38MAPK expression in a CCI rat model","volume":"21","author":"Liu R","year":"2018","unstructured":"Liu R, Xu X, Xu Y, Fang X, Lin X. Pulsed radiofrequency on dorsal root ganglion relieved neuropathic pain associated with downregulation of the spinal interferon regulatory factor 8, microglia, p38MAPK expression in a CCI rat model. Pain Physician 2018; 21(4): E307\u2013E322.","journal-title":"Pain Physician"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2025.115034"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2012.02.014"},{"issue":"2","key":"e_1_3_3_17_2","first-page":"113","article-title":"Effects of intrathecal injection of IRF8 SiRNA on pain threshold and activation of spinal cord microglia in rats with postoperative persistent pain","volume":"38","author":"Xu CS","year":"2022","unstructured":"Xu CS, Lin C, Cai ZY. Effects of intrathecal injection of IRF8 SiRNA on pain threshold and activation of spinal cord microglia in rats with postoperative persistent pain. 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