{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T15:05:40Z","timestamp":1784041540680,"version":"3.55.0"},"reference-count":60,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"the Shenzhen Municipal Science and Technology Program Project","award":["JCYJ20220530162614031"],"award-info":[{"award-number":["JCYJ20220530162614031"]}]},{"name":"the Traditional Chinese Medicine Research Project of Guangdong Provincial Administration of Traditional Chinese Medicine","award":["20252038"],"award-info":[{"award-number":["20252038"]}]},{"name":"the Medical and Health Research Project of Bao'an District Medical Association in Shenzhen","award":["BAYXY2024041"],"award-info":[{"award-number":["BAYXY2024041"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Mol Pain"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>This study aimed to explore potential biomarkers and mechanisms underlying in the treatment of neuropathic pain (NP) with Fu\u2019s subcutaneous needling (FSN), employing a transcriptomics approach.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>In this study, RNA sequencing was performed in a rat model of chronic constriction injury treated with FSN and acupuncture methods. The FSN-related genes in the treatment of NP with FSN were obtained by overlapping the results of differential expression analysis. Potential biomarkers were identified by protein-protein interaction (PPI) analysis and machine learning. In addition, potential biomarkers were analyzed for functional enrichment, molecular regulatory networks, and drug prediction. Reverse transcription quantitative PCR (RT-qPCR) was employed to validate the expression levels of the biomarkers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>FOS, RAC2, and TYROBP were identified as potential biomarkers. Furthermore, mo-miR-92a-2-5p was predicted to co-target RAC2 and TYROBP. Twenty-five drugs were predicted to target FOS and four drugs were predicted to target RAC2 were also identified. FSN treatment enhanced and attenuated the expression of RAC2, TYROBP, and FOS, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>In this study, FOS, RAC2, and TYROBP were identified as potential biomarkers in the treatment of NP with FSN, providing a potential theoretical basis for NP treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/17448069261459255","type":"journal-article","created":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T05:29:14Z","timestamp":1780550954000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptome profiling and experimental validation identify FOS, RAC2, and TYROBP as potential biomarkers for Fu\u2019s subcutaneous needling in neuropathic pain treatment"],"prefix":"10.1177","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-0061-5707","authenticated-orcid":false,"given":"Huiqin","family":"Fang","sequence":"first","affiliation":[{"name":"Neurology Department, Qingdao Hiser Hospital Affiliated of Qingdao University (Qingdao Traditional Chinese Medicine Hospital), Qingdao, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3435-2655","authenticated-orcid":false,"given":"Yaping","family":"Li","sequence":"additional","affiliation":[{"name":"Encephalopathy Rehabilitation Department, Weifang Traditional Chinese Medicine Hospital, Weifang, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Li","sequence":"additional","affiliation":[{"name":"General Medicine, Bao\u2019an Authentic TCM Therapy Hospital, Shenzhen, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8306-8441","authenticated-orcid":false,"given":"Yarong","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Integrated Medicine, Qingdao University, Qingdao, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7651-7692","authenticated-orcid":false,"given":"Yunhua","family":"Zang","sequence":"additional","affiliation":[{"name":"Clinical Division of the School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,6,3]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2011.06.017"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2024.107076"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12325-020-01388-w"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu12082221"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00702-020-02145-7"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.756940"},{"key":"e_1_3_2_8_2","first-page":"196","article-title":"Efficacy of Fu\u2019s subcutaneous needling on sciatic nerve pain: behavioral and electrophysiological changes in a chronic constriction injury rat model","volume":"30","author":"Chiu PE","year":"2023","unstructured":"Chiu PE, Fu Z, Lai DW, Chou LW. 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