{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T13:38:17Z","timestamp":1792849097773,"version":"4.5.0"},"reference-count":33,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["NS109059"],"award-info":[{"award-number":["NS109059"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE018661"],"award-info":[{"award-number":["DE018661"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE023090"],"award-info":[{"award-number":["DE023090"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Mol Pain"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:p>\n                    Orofacial neuropathic pain is a debilitating pain condition. It is poorly treated, partially due to an incomplete understanding of its underlying neurophysiological mechanisms. Orofacial neuropathic pain is often caused by injury or dysfunction of trigeminal nerve branches, including the infraorbital nerve (IoN). Clinically, a main symptom of orofacial neuropathic pain is mechanical allodynia, excruciating pain induced by gentle touch. While previous studies on neuropathic pain have mainly focused on the involvement of C- and A\u03b4-afferents, the potential contribution of A\u03b2-afferents to orofacial neuropathic pain has not been well explored. In the present study, we have established a mouse model of chronic constrictive injury of the IoN (IoN-CCI) and assessed orofacial pain behaviors and excitability of A\u03b2-afferent trigeminal ganglion (TG) neurons. Behavioral assessment using the von Frey test has revealed significantly increased mechanical sensitivity 3-4 weeks post-surgery in IoN-CCI mice compared with the sham group. Machine-learning-based eyelid distance measurement has demonstrated orbital tightening in IoN-CCI mice, suggesting spontaneous pain. Using\n                    <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                    TG preparations and patch-clamp recordings, we have found that action potential (AP) thresholds are significantly reduced in both non-nociceptive-like and nociceptive-like A\u03b2-afferent TG neurons of IoN-CCI mice. Consistently, there is a significant increase in voltage-gated Na\n                    <jats:sup>+<\/jats:sup>\n                    currents in both non-nociceptive-like and nociceptive-like A\u03b2-afferent TG neurons following IoN-CCI. Our findings demonstrate that both non-nociceptive-like and nociceptive-like A\u03b2-afferent TG neurons undergo hyperexcitability following infraorbital nerve injury, raising the possibility that both types of A\u03b2-afferent TG neurons may contribute to orofacial neuropathic pain following infraorbital nerve injury.\n                  <\/jats:p>","DOI":"10.1177\/17448069261499167","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T21:19:53Z","timestamp":1792531193000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Orofacial neuropathic pain and enhanced excitability of non-nociceptive-like and nociceptive-like trigeminal A\u03b2-afferent neurons in mice following infraorbital nerve chronic constrictive injury"],"prefix":"10.1177","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-7385-7328","authenticated-orcid":false,"given":"Tatsuo","family":"Ueda","sequence":"first","affiliation":[{"name":"University of Alabama at Birmingham"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saurav","family":"Gupta","sequence":"additional","affiliation":[{"name":"University of Alabama at Birmingham"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit Raj","family":"Sharma","sequence":"additional","affiliation":[{"name":"University of Alabama at Birmingham"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Ling","sequence":"additional","affiliation":[{"name":"University of Alabama at Birmingham"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8404-9850","authenticated-orcid":false,"given":"Jianguo G.","family":"Gu","sequence":"additional","affiliation":[{"name":"University of Alabama at Birmingham"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,10,20]]},"reference":[{"issue":"2","key":"e_1_3_4_2_2","first-page":"129","article-title":"1st edition (ICOP)","author":"International Classification of Orofacial Pain","year":"2020","unstructured":"International Classification of Orofacial Pain. 1st edition (ICOP). Cephalalgia 2020; 2: 129\u2013221.40.","journal-title":"Cephalalgia"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/0333102417706310"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2021.691396"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.sult.2022.04.002"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.5603\/FM.2015.0025"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13041-023-01015-z"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/17448069241240452"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2014.01.012"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1997.78.4.1841"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13250"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2004.01.007"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1113\/JP276608"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13251"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2004.07.015"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2013.07.051"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2016.05.008"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00052.2017"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1177\/17448069221148958"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(14)70102-4"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2659-06.2006"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012944"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(88)90027-9"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-48095-6"},{"key":"e_1_3_4_25_2","article-title":"Chronic Constriction Injury of the Distal Infraorbital Nerve (DIoN-CCI) in Mice to Study Trigeminal Neuropathic Pain","volume":"205","author":"Deseure KR","year":"2024","unstructured":"Deseure KR, Hans GH. Chronic Constriction Injury of the Distal Infraorbital Nerve (DIoN-CCI) in Mice to Study Trigeminal Neuropathic Pain. J Vis Exp 2024; 205. https:\/\/doi.org\/10.3791\/66420","journal-title":"J Vis Exp"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1177\/17448069251410828"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ynpai.2024.100164"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1177\/17448069241261687"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2019.02.024"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1455"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2016.10.011"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2203-07.2007"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13041-018-0384-5"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00065.2011"}],"container-title":["Molecular Pain"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/17448069261499167","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/17448069261499167","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/17448069261499167","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T13:20:26Z","timestamp":1792848026000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/17448069261499167"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":33,"alternative-id":["10.1177\/17448069261499167"],"URL":"https:\/\/doi.org\/10.1177\/17448069261499167","relation":{},"ISSN":["1744-8069","1744-8069"],"issn-type":[{"value":"1744-8069","type":"print"},{"value":"1744-8069","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"article-number":"17448069261499167"}}