{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:36:14Z","timestamp":1792787774926,"version":"4.5.0"},"reference-count":32,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Shaanxi Provincial Administration of Traditional Chinese Medicine","award":["SZY-KJCYC-2025-LC-010"],"award-info":[{"award-number":["SZY-KJCYC-2025-LC-010"]}],"id":[{"id":"https:\/\/ror.org\/03x55t826","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Mol Pain"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The effect of Gelsemine on neuropathic pain was investigated using a sciatic nerve (SN) chronic constriction injury (CCI) rat model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Network pharmacology analysis was performed to identify potential molecular targets of Gelsemine associated with neuropathic pain, followed by molecular docking analysis of the identified targets. Neuropathic pain was induced in male rats by SN-CCI. The rats were divided into four groups: sham, CCI, Gelsemine 2 mg\/kg, and Gelsemine 4 mg\/kg. Gelsemine (2 or 4 mg\/kg, i.p.) was administered to the treatment groups for 30 consecutive days. The effect of Gelsemine on mechanical hypersensitivity was evaluated, and the BBB score was used as a secondary assessment of general locomotor function following CCI. The levels and expression of inflammatory mediators were analyzed by ELISA and qRT-PCR, respectively, and the expression of THIK1, caspase-8, and cytosolic phospholipase A2 (cPLA2) in nerve tissue was analyzed by western blot.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Network pharmacology analysis identified ASK1 and TrkB (NTRK2) as potential molecular targets of Gelsemine in neuropathic pain, and molecular docking predicted favorable binding interactions with these proteins. Gelsemine significantly attenuated mechanical hypersensitivity and improved BBB locomotor scores in CCI rats. It also reduced the altered serum levels of IL-4, IL-6, IL-1\u03b2, and TNF-\u03b1, as well as the mRNA expression of NF-\u03baB, IL-6, and TNF-\u03b1, compared with the CCI group. Furthermore, Gelsemine was associated with significantly reduced mRNA expression of ASK1, TrkB (NTRK2), and MAPK1 in SN tissue and normalization of the altered expression of THIK1, caspase-8, and cPLA2 in male CCI rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Gelsemine alleviated mechanical hypersensitivity and improved locomotor function, while ameliorating inflammatory and oxidative stress-related changes in male CCI rats. Computational analyses identified ASK1 and TrkB (NTRK2) as potential molecular targets, while Gelsemine treatment was associated with reduced expression of ASK1, TrkB, and MAPK1. These findings suggest a possible association between Gelsemine treatment and ASK1\/TrkB\/MAPK-related signaling; however, further functional studies are required to establish their causal involvement in the analgesic effects of Gelsemine. Only male rats were included in this study; therefore, the findings are limited to male physiology and cannot be generalized to female animals.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/17448069261499174","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T18:57:15Z","timestamp":1792522635000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Gelsemine attenuates neuropathic pain in a rat model of sciatic nerve chronic constriction injury"],"prefix":"10.1177","volume":"22","author":[{"given":"Yao","family":"Lu","sequence":"first","affiliation":[{"name":"Northwest Women\u2019s and Children\u2019s Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9292-7593","authenticated-orcid":false,"given":"Dandan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Northwest Women\u2019s and Children\u2019s Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaik","family":"Althaf Hussain","sequence":"additional","affiliation":[{"name":"King Saud University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alok Shiomurti","family":"Tripathi","sequence":"additional","affiliation":[{"name":"ERA College of Pharmacy"},{"name":"ERA University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,10,20]]},"reference":[{"issue":"3","key":"e_1_3_6_2_2","first-page":"249","article-title":"Neuropathic pain and chronic pain as an underestimated interdisciplinary problem","volume":"35","author":"Szewczyk AK","year":"2022","unstructured":"Szewczyk AK, Jamroz-Wi\u015bniewska A, Haratym N, et al. Neuropathic pain and chronic pain as an underestimated interdisciplinary problem. Int J Occup Med Environ Health 2022; 35(3): 249\u2013264. https:\/\/doi.org\/10.13075\/ijomeh.1896.01676","journal-title":"Int J Occup Med Environ Health"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2010.08.025"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2017.06.010"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2012.01.002"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.5812\/aapm.113020"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/glia.24174"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-024-03042-6"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-020-05522-y"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/brainsci12020191"},{"issue":"5","key":"e_1_3_6_11_2","first-page":"1305","article-title":"Gelsemine Exerts Neuroprotective Effects on Neonatal Mice with Hypoxic-Ischemic Brain Injury by Suppressing Inflammation and Oxidative Stress via Nrf2\/HO-1 Pathway","volume":"48","author":"Cheng S","year":"2023","unstructured":"Cheng S, Chen C, Wang L. Gelsemine Exerts Neuroprotective Effects on Neonatal Mice with Hypoxic-Ischemic Brain Injury by Suppressing Inflammation and Oxidative Stress via Nrf2\/HO-1 Pathway. Neurochem Res 2023; 48(5): 1305\u20131319.","journal-title":"Neurochem Res"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2013.07.027"},{"key":"e_1_3_6_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2015.86"},{"key":"e_1_3_6_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12013-014-0203-2"},{"key":"e_1_3_6_15_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2004000300017"},{"key":"e_1_3_6_16_2","doi-asserted-by":"publisher","DOI":"10.1089\/neu.1995.12.1"},{"key":"e_1_3_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(79)90738-3"},{"key":"e_1_3_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)45228-9"},{"key":"e_1_3_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(68)90092-4"},{"key":"e_1_3_6_20_2","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12041380"},{"key":"e_1_3_6_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-019-02850-0"},{"key":"e_1_3_6_22_2","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000000492"},{"key":"e_1_3_6_23_2","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/5160261"},{"issue":"5","key":"e_1_3_6_24_2","first-page":"587","article-title":"Prolonged constriction of sciatic nerve affecting oxidative stressors & antioxidant enzymes in rat","volume":"129","author":"Varija D","year":"2009","unstructured":"Varija D, Kumar KP, Reddy KP, et al. Prolonged constriction of sciatic nerve affecting oxidative stressors & antioxidant enzymes in rat. Indian J Med Res 2009; 129(5): 587\u2013592.","journal-title":"Indian J Med Res"},{"key":"e_1_3_6_25_2","doi-asserted-by":"publisher","DOI":"10.3390\/jpm13040626"},{"key":"e_1_3_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2008.03.035"},{"key":"e_1_3_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cotox.2017.11.002"},{"key":"e_1_3_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpain.2008.09.009"},{"key":"e_1_3_6_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/cns.14807"},{"key":"e_1_3_6_30_2","doi-asserted-by":"publisher","DOI":"10.2183\/pjab.88.434"},{"key":"e_1_3_6_31_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms27020984"},{"key":"e_1_3_6_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-022-04602-1"},{"key":"e_1_3_6_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2023.09.037"}],"container-title":["Molecular Pain"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/17448069261499174","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/17448069261499174","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/17448069261499174","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:22:04Z","timestamp":1792675324000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/17448069261499174"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":32,"alternative-id":["10.1177\/17448069261499174"],"URL":"https:\/\/doi.org\/10.1177\/17448069261499174","relation":{},"ISSN":["1744-8069","1744-8069"],"issn-type":[{"value":"1744-8069","type":"print"},{"value":"1744-8069","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"article-number":"17448069261499174"}}