{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T13:40:40Z","timestamp":1791034840735,"version":"4.1.0"},"reference-count":32,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2011,10,26]],"date-time":"2011-10-26T00:00:00Z","timestamp":1319587200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Innate Immun"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:p>\n                    Onsets of bacterial infections devastate the compromised immune system in AIDS patients. Damaged gut mucosa permits dissemination of bacterial toxins into deeper layers and hyper-activation of the immune system. We previously reported that the unfractionated supernatants of HIV-resistant CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells impeded the NF-\u03baB\/DNA binding in macrophages induced by either HIV-1 or LPS. The active component of this soluble material was identified as X-DING-CD4 (extracellular DING from CD4 T cells). We hypothesized that the anti-inflammatory effect of the X-DING-CD4 protein might extend to non-immune cells, for example endothelial cells, undergoing persistent endotoxin stimulation in the course of advanced HIV disease. To test this proposition, we evaluated the efficiency of NF-\u03baB and Ap-1 binding to the IL-8 promoter in LPS-activated endothelial cells and control human macrophages exposed to native X-DING-CD4 protein. We found a deficiency of NF-\u03baB- but not AP-1-DNA binding in the systems where cells were treated with native soluble X-DING-CD4 protein. The X-DING-CD4-mediated inhibition of the IL-8 promoter also resulted in a reduction of the soluble IL-8 protein in endothelial cells and human macrophages infected with a subset of enteric bacteria frequently causing diarrhea in progressive HIV disease . Bacterial endotoxin did not induce the endogenous X-DING-CD4 mRNA activity in human macrophages and transformed CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells, indicating that the reduction of LPS-mediated IL-8 promoter activation was not related to de novo X-DING-CD4 protein synthesis, but depended on function of the exogenous X-DING-CD4 protein. This study provides evidence that the X-DING-CD4 protein might be developed as a novel biotherapeutic to control LPS-mediated inflammation in advanced HIV disease.\n                  <\/jats:p>","DOI":"10.1177\/1753425911427065","type":"journal-article","created":{"date-parts":[[2011,10,26]],"date-time":"2011-10-26T20:59:41Z","timestamp":1319662781000},"page":"571-579","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Native X-DING-CD4 protein secreted by HIV-1 resistant CD4\n                    <sup>+<\/sup>\n                    T cells blocks activity of IL-8 promoter in human endothelial cells infected with enteric bacteria"],"prefix":"10.1177","volume":"18","author":[{"given":"Anna","family":"Ivanova","sequence":"first","affiliation":[{"name":"Protein Chemistry Laboratory, St. Luke\u2019s\/Roosevelt Hospital, Columbia University, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasheda Y","family":"Shilpi","sequence":"additional","affiliation":[{"name":"Protein Chemistry Laboratory, St. Luke\u2019s\/Roosevelt Hospital, Columbia University, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakhee","family":"Sachdeva","sequence":"additional","affiliation":[{"name":"Protein Chemistry Laboratory, St. Luke\u2019s\/Roosevelt Hospital, Columbia University, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanhua","family":"Li","sequence":"additional","affiliation":[{"name":"Protein Chemistry Laboratory, St. Luke\u2019s\/Roosevelt Hospital, Columbia University, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malgorzata","family":"Simm","sequence":"additional","affiliation":[{"name":"Protein Chemistry Laboratory, St. Luke\u2019s\/Roosevelt Hospital, Columbia University, New York, 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