{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T12:46:23Z","timestamp":1780577183608,"version":"3.54.1"},"reference-count":50,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2012,10,29]],"date-time":"2012-10-29T00:00:00Z","timestamp":1351468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Innate Immun"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:p>We showed recently that repeated oral exposure to LPS stimulated humoral immune responses in periparturient dairy cows. Here, metabolic and mineral responses to repeated oral administration of LPS were investigated. Sixteen clinically healthy, pregnant Holstein cows were orally administered 3\u2009ml of saline solution (control) or 3\u2009ml of saline solution containing 3 increasing doses of LPS, at 07:00\u2009h, as follows: (i) 0.01\u2009\u00b5g\/kg body mass (BM) on d \u221214 and \u221210, (ii) 0.05\u2009\u00b5g\/kg BM on d\u2009\u22127 and \u22123, and (iii) 0.1\u2009\u00b5g\/kg BM on d\u20093 and 7 relative to parturition. Blood samples were measured shortly before, and at 8 different time-points after (up to 6\u2009h), the first challenge of each LPS dosage to evaluate the post-challenge plasma profile, as well as weekly up to 4 wk postpartum. Results showed that oral administration of LPS lowered concentrations of non-esterified fatty acids ( P\u2009&lt;\u20090.01) and \u03b2-hydroxy-butyrate ( P\u2009&lt;\u20090.01) in the plasma, particularly after the third LPS challenge. Also, after the third oral LPS challenge, treatment tended to increase plasma glucose. Plasma calcium did not change, but concentrations of insulin ( P\u2009&lt;\u20090.01) and zinc ( P\u2009&lt;\u20090.01) were greater, while that of copper was lower ( P\u2009&lt;\u20090.01) in the plasma of treated cows. This is the first report to indicate a potential role for repeated oral administration of LPS around parturition to modulate the profile of plasma metabolites and minerals postpartum.<\/jats:p>","DOI":"10.1177\/1753425912461287","type":"journal-article","created":{"date-parts":[[2012,10,30]],"date-time":"2012-10-30T00:48:40Z","timestamp":1351558120000},"page":"298-314","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Oral challenge with increasing doses of LPS modulated the patterns of plasma metabolites and minerals in periparturient dairy cows"],"prefix":"10.1177","volume":"19","author":[{"given":"Qendrim","family":"Zebeli","sequence":"first","affiliation":[{"name":"Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada"},{"name":"Institute of Animal Nutrition, Department for Farm Animals and Veterinary Public Health, Vetmeduni Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominik","family":"Mansmann","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanti","family":"Sivaraman","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanna M","family":"Dunn","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burim N","family":"Ametaj","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2012,10,29]]},"reference":[{"key":"bibr1-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1038\/nri1004"},{"key":"bibr2-1753425912461287","first-page":"43","volume":"86","author":"Greisman SE","year":"1975","journal-title":"Trans Am Clin Climatol Assoc"},{"key":"bibr3-1753425912461287","doi-asserted-by":"crossref","unstructured":"Ametaj BN, Sivaraman S, Dunn SM, et al. Repeated oral administration of lipopolysaccharide from\n                      Escherichia coli\n                      0111:B4 modulated humoral immune responses in periparturient dairy cows.\n                      Innate Immun\n                      2012; 18: 638-647.","DOI":"10.1177\/1753425911434851"},{"key":"bibr4-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(03)73949-6"},{"key":"bibr5-1753425912461287","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2007-0634"},{"key":"bibr6-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2011-4194"},{"key":"bibr7-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1177\/1753425908099170"},{"key":"bibr8-1753425912461287","doi-asserted-by":"publisher","DOI":"10.2527\/1999.7792523x"},{"key":"bibr9-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(96)90073-9"},{"key":"bibr10-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(03)73948-4"},{"key":"bibr11-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(06)72123-3"},{"key":"bibr12-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(03)73825-9"},{"key":"bibr13-1753425912461287","doi-asserted-by":"crossref","unstructured":"Petzl W, G\u00fcnther J, Pfister T, et al. Lipopolysaccharide pretreatment of the udder protects against experimental Escherichia coli mastitis.\n                      Innate Immun\n                      2012; 18(3): 467-477.","DOI":"10.1177\/1753425911422407"},{"key":"bibr14-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-007-6116-4"},{"key":"bibr15-1753425912461287","volume-title":"Guide to the care and use of experimental animals","volume":"1","author":"Canadian Council on Animal Care (CCAC)","year":"1993","edition":"2"},{"key":"bibr16-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2008-1465"},{"key":"bibr17-1753425912461287","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2009-2203"},{"key":"bibr18-1753425912461287","volume-title":"SAS Online Doc., 9.1.3","author":"SAS Institute Inc","year":"2002"},{"key":"bibr19-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(03)73796-5"},{"key":"bibr20-1753425912461287","first-page":"121","volume":"20","author":"Brockman RP","year":"1979","journal-title":"Can Vet J"},{"key":"bibr21-1753425912461287","doi-asserted-by":"publisher","DOI":"10.4141\/A04-043"},{"key":"bibr22-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2008-1507"},{"key":"bibr23-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2008-1634"},{"key":"bibr24-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2007-0256"},{"key":"bibr25-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00739-09"},{"key":"bibr26-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199902113400607"},{"key":"bibr27-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(03)73664-9"},{"key":"bibr28-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3945\/jn.109.108233"},{"key":"bibr29-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2008-1454"},{"key":"bibr30-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(01)70204-4"},{"key":"bibr31-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607076103"},{"key":"bibr32-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115381"},{"key":"bibr33-1753425912461287","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.8.5399"},{"key":"bibr34-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2011-5188"},{"key":"bibr35-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1097\/00075197-200209000-00015"},{"key":"bibr36-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.1056S22"},{"key":"bibr37-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(90)90033-R"},{"key":"bibr38-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2009.02.024"},{"key":"bibr39-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1440109"},{"key":"bibr40-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2007.07.006"},{"key":"bibr41-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2007.10.001"},{"key":"bibr42-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2010-3118"},{"key":"bibr43-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(03)73708-4"},{"key":"bibr44-1753425912461287","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(90)79010-8"},{"key":"bibr45-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/5.1.137"},{"key":"bibr46-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.8.3137"},{"key":"bibr47-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1007\/BF01965394"},{"key":"bibr48-1753425912461287","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1985.65.2.238"},{"key":"bibr49-1753425912461287","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.2460\/ajvr.1981.42.07.1109","volume":"42","author":"Feldman BF","year":"1981","journal-title":"Am J Vet Res"},{"key":"bibr50-1753425912461287","first-page":"1259","volume":"18","author":"Ramadori G","year":"1998","journal-title":"Comparison with interleukin 1\u03b2 and tumor necrosis factor-\u03b1. European J Immunol"}],"container-title":["Innate Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1753425912461287","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1753425912461287","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1753425912461287","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:01:17Z","timestamp":1777460477000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1753425912461287"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,29]]},"references-count":50,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1177\/1753425912461287"],"URL":"https:\/\/doi.org\/10.1177\/1753425912461287","relation":{},"ISSN":["1753-4259","1753-4267"],"issn-type":[{"value":"1753-4259","type":"print"},{"value":"1753-4267","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,29]]}}}