{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T14:14:20Z","timestamp":1777731260849,"version":"3.51.4"},"reference-count":46,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2012,12,4]],"date-time":"2012-12-04T00:00:00Z","timestamp":1354579200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Innate Immun"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:p>Alterations in innate immunity that predispose to chronic obstructive pulmonary disease (COPD) exacerbations are poorly understood. We examined innate immunity gene expression in peripheral blood polymorphonuclear leukocytes (PMN) and monocytes stimulated by Haemophilus influenzae and Streptococcus pneumoniae. Thirty COPD patients (15 rapid and 15 non-rapid lung function decliners) and 15 smokers without COPD were studied. Protein expression of IL-8, IL-6, TNF-\u03b1 and IFN-\u03b3 (especially monocytes) increased with bacterial challenge. In monocytes stimulated with S. pneumoniae, TNF-\u03b1 protein expression was higher in COPD (non-rapid decliners) than in smokers. In co-cultures of monocytes and PMN, mRNA expression of TGF-\u03b21 and MYD88 was up-regulated, and CD14, TLR2 and IFN-\u03b3 down-regulated with H. influenzae challenge. TNF-\u03b1 mRNA expression was increased with H. influenzae challenge in COPD. Cytokine responses were similar between rapid and non-rapid decliners. TNF-\u03b1 expression was up-regulated in non-rapid decliners in response to H. influenzae (monocytes) and S. pneumoniae (co-culture of monocytes and PMN). Exposure to bacterial pathogens causes characteristic innate immune responses in peripheral blood monocytes and PMN in COPD. Bacterial exposure significantly alters the expression of TNF-\u03b1 in COPD patients, although not consistently. There did not appear to be major differences in innate immune responses between rapid and non-rapid decliners.<\/jats:p>","DOI":"10.1177\/1753425912466926","type":"journal-article","created":{"date-parts":[[2012,12,5]],"date-time":"2012-12-05T13:53:24Z","timestamp":1354715604000},"page":"428-437","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Peripheral compartment innate immune response to\n                    <i>Haemophilus influenzae<\/i>\n                    and\n                    <i>Streptococcus pneumoniae<\/i>\n                    in chronic obstructive pulmonary disease patients"],"prefix":"10.1177","volume":"19","author":[{"given":"Santiyagu M Savarimuthu","family":"Francis","sequence":"first","affiliation":[{"name":"Department of Thoracic Medicine, The Prince Charles Hospital, Brisbane, QLD, Australia"},{"name":"School of Medicine, The University of Queensland, Brisbane, QLD, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maxine E","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, The Prince Charles Hospital, Brisbane, QLD, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela R","family":"Fung","sequence":"additional","affiliation":[{"name":"School of Medicine, The University of Queensland, Brisbane, QLD, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet G","family":"Shaw","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, The Prince Charles Hospital, Brisbane, QLD, Australia"},{"name":"School of Medicine, The University of Queensland, Brisbane, QLD, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annalese BT","family":"Semmler","sequence":"additional","affiliation":[{"name":"Institute of Health and Biomedical Innovation, Queensland University of Technology Brisbane, QLD, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Nataatmadja","sequence":"additional","affiliation":[{"name":"School of Medicine, The University of Queensland, Brisbane, QLD, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rayleen V","family":"Bowman","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, The Prince Charles Hospital, Brisbane, QLD, Australia"},{"name":"School of Medicine, The University of Queensland, Brisbane, QLD, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwun M","family":"Fong","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, The Prince Charles Hospital, Brisbane, QLD, Australia"},{"name":"School of Medicine, The University of Queensland, Brisbane, QLD, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian A","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, The Prince Charles Hospital, Brisbane, QLD, Australia"},{"name":"School of Medicine, The University of Queensland, Brisbane, QLD, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2012,12,4]]},"reference":[{"key":"bibr1-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0909883"},{"key":"bibr2-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2009.01702.x"},{"key":"bibr3-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1053\/rmed.2002.1334"},{"key":"bibr4-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1378\/chest.06-1610"},{"key":"bibr5-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1105482"},{"key":"bibr6-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201104-0597OC"},{"key":"bibr7-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016384"},{"key":"bibr8-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(00)00507-6"},{"key":"bibr9-1753425912466926","doi-asserted-by":"publisher","DOI":"10.2147\/COPD.S15417"},{"key":"bibr10-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1016\/j.arbres.2010.12.003"},{"key":"bibr11-1753425912466926","doi-asserted-by":"publisher","DOI":"10.2147\/COPD.S1210"},{"key":"bibr12-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)60988-4"},{"key":"bibr13-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006860"},{"key":"bibr14-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00061911"},{"key":"bibr15-1753425912466926","unstructured":"World Health Organization (WHO).\n                      Global strategy for the diagnosis, management, and prevention of chronic obstructive pumonary disease.\n                      Geneva: WHO, 2011"},{"key":"bibr16-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.05.00034805"},{"key":"bibr17-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gene.6363961"},{"key":"bibr18-1753425912466926","first-page":"745","volume":"23","author":"Oh H","year":"2008","journal-title":"J Vis Exp"},{"key":"bibr19-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.58.4.348"},{"key":"bibr20-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-6-151"},{"key":"bibr21-1753425912466926","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.5.2610"},{"key":"bibr22-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.8.3714-3718.1999"},{"key":"bibr23-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1105626"},{"key":"bibr24-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"bibr25-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00036709"},{"key":"bibr26-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2006.00890.x"},{"key":"bibr27-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.10.5803-5813.2003"},{"key":"bibr28-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.231625398"},{"key":"bibr29-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.10.1.25-29.1974"},{"key":"bibr30-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.163.2.2003122"},{"key":"bibr31-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1159\/000075648"},{"key":"bibr32-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1513\/pats.200512-125SF"},{"key":"bibr33-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.17.8.1203"},{"key":"bibr34-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-7-64"},{"key":"bibr35-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1177\/1753425909106317"},{"key":"bibr36-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018426"},{"key":"bibr37-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1378\/chest.128.4.1995"},{"key":"bibr38-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-4-11"},{"key":"bibr39-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2350-10-23"},{"key":"bibr40-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.153.2.8564110"},{"key":"bibr41-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031976"},{"key":"bibr42-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa063070"},{"key":"bibr43-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)61298-8"},{"key":"bibr44-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1177\/1753425911423270"},{"key":"bibr45-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200509-1461OC"},{"key":"bibr46-1753425912466926","doi-asserted-by":"publisher","DOI":"10.1159\/000324297"}],"container-title":["Innate Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1753425912466926","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1753425912466926","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1753425912466926","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:01:19Z","timestamp":1777460479000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1753425912466926"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12,4]]},"references-count":46,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["10.1177\/1753425912466926"],"URL":"https:\/\/doi.org\/10.1177\/1753425912466926","relation":{},"ISSN":["1753-4259","1753-4267"],"issn-type":[{"value":"1753-4259","type":"print"},{"value":"1753-4267","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12,4]]}}}