{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T21:53:11Z","timestamp":1789854791653,"version":"4.0.1"},"reference-count":73,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2014,9,18]],"date-time":"2014-09-18T00:00:00Z","timestamp":1410998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Innate Immun"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:p>The severity of systemic meningococcal disease (SMD) correlates to plasma concentrations of LPS and IL-10, with the highest levels detected in non-survivors. Here, plasma from patients with SMD containing high and low concentrations of LPS were incubated with human monocytes before and after immunodepletion of IL-10 to study the effect of IL-10 on gene expression and cytokine release. Patient plasma containing IL-10 induced the expression of 1657 genes in human monocytes when compared with gene expression induced by low LPS plasma. After immunodepletion of IL-10, this number increased to 2260. By directly comparing the gene expression profiles induced before and after immunodepletion of IL-10, the presence of IL-10 differentially regulated 373 genes. Functional classes associated with these genes were cellular function and maintenance, cellular development, cellular growth and proliferation, cell\u2013cell signaling and interaction and cellular movement. Immunodepletion of IL-10 resulted in down-regulation of genes of the leukocyte immunoglobulin-like receptor family, and up-regulation of genes of type I IFN signaling, TLR signaling, the inflammasomes, coagulation and fibrinolysis. Finally, immunodepletion of IL-10 increased the protein levels of IL-1\u03b2, IL-8, TNF-\u03b1, MIP-1\u03b1 and MIP-1\u03b2. Data suggest that IL-10 in meningococcal sepsis plasma regulates a variety of genes and signaling pathways, likely leading to an overall inhibitory effect on the inflammatory response induced in meningococcal sepsis.<\/jats:p>","DOI":"10.1177\/1753425914547743","type":"journal-article","created":{"date-parts":[[2014,9,18]],"date-time":"2014-09-18T23:59:16Z","timestamp":1411084756000},"page":"429-449","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":16,"title":["IL-10 immunodepletion from meningococcal sepsis plasma induces extensive changes in gene expression and cytokine release in stimulated human monocytes"],"prefix":"10.1177","volume":"21","author":[{"given":"Unni","family":"Gopinathan","sequence":"first","affiliation":[{"name":"Blood Cell Research Group, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, University of Oslo, Oslo, Norway"},{"name":"Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berit Sletbakk","family":"Brusletto","sequence":"additional","affiliation":[{"name":"Blood Cell Research Group, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ole Kristoffer","family":"Olstad","sequence":"additional","affiliation":[{"name":"Blood Cell Research Group, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Kierulf","sequence":"additional","affiliation":[{"name":"Blood Cell Research Group, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, University of Oslo, Oslo, Norway"},{"name":"Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens Petter","family":"Berg","sequence":"additional","affiliation":[{"name":"Blood Cell Research Group, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, University of Oslo, Oslo, Norway"},{"name":"Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petter","family":"Brandtzaeg","sequence":"additional","affiliation":[{"name":"Blood Cell Research Group, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, University of Oslo, Oslo, Norway"},{"name":"Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway"},{"name":"Department of Pediatrics, Oslo University Hospital, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reidun","family":"\u00d8vsteb\u00f8","sequence":"additional","affiliation":[{"name":"Blood Cell Research Group, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2014,9,18]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)17667-8"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(13)70001-X"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/adc.86.1.44"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.7.2980-2987.2004"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.1.144"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61016-2"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-346-2_2"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/159.2.195"},{"key":"e_1_3_4_10_2","first-page":"401","article-title":"Neisseria meningitidis lipopolysaccharides in human pathology","volume":"7","author":"Brandtzaeg P","year":"2001","unstructured":"Brandtzaeg P, Bjerre A, \u00d8vsteb\u00f8 R, . Neisseria meningitidis lipopolysaccharides in human pathology. J Endotoxin Res 2001; 7: 401\u2013420.","journal-title":"J Endotoxin Res"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/171.1.229"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.6.2109-2112.1995"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.1.51"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e3181692c0b"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e31819b52fd"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e31819fcc08"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/cc9392"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01625-07"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00386-12"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.72.4.800"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.5.2253"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2005.05.003"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200324323"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.290040305"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e3181844399"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1080\/003655100750019260"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1177\/09680519030090030301"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e3181de8c94"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.70.2.283"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0001-2815.2004.00290.x"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-009-8148-8"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215254"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.10.6596"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.412643"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12040"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1001650"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1300157"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1307419110"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01505-08"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2004.08.015"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.182.1.588"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-0106-3_14"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200636038"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3133"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3344"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni910"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.5613"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033478"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.160.7.3096"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199811)28:11<3423::AID-IMMU3423>3.0.CO;2-2"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-05-089771"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199807)28:07<2086::AID-IMMU2086>3.0.CO;2-T"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0907609"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10759"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000661"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-03-146720"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02664"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.02.004"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2001-18866"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1254046"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(90)90326-8"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1160\/TH08-11-0735"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.8.2701"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1975.tb00547.x"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.3.930.003k46_930_935"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)81693-T"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1160\/TH06-11-0622"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1177\/1753425911428230"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V85.5.1341.bloodjournal8551341"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115493"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00184-08"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2011.1829"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-200302000-00004"}],"container-title":["Innate Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1753425914547743","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1753425914547743","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1753425914547743","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:02:02Z","timestamp":1777460522000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1753425914547743"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,18]]},"references-count":73,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["10.1177\/1753425914547743"],"URL":"https:\/\/doi.org\/10.1177\/1753425914547743","relation":{},"ISSN":["1753-4259","1753-4267"],"issn-type":[{"value":"1753-4259","type":"print"},{"value":"1753-4267","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,18]]}}}