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The control and LPS groups were fed a basal diet, and the LPS\u2009+\u2009anemonin group received the basal diet\u2009+\u2009100\u2009mg anemonin\/kg diet. After 21\u2009d of feeding, the LPS- and anemonin-treated piglets received i.p. administration of LPS; the control group received saline. At 4\u2009h post-injection, jejunum samples were collected. The results showed that supplemental anemonin increased villus height and transepithelial electrical resistance, and decreased crypt depth and paracellular flux of dextran (4\u2009kDa) compared with the LPS group. Moreover, anemonin increased tight junction claudin-1, occludin and ZO-1 expression in the jejunal mucosa, compared with LPS group. Anemonin also decreased TNF-\u03b1, IL-6, IL-8 and IL-1\u03b2 mRNA expression. Supplementation with anemonin also increased TGF-\u03b21 mRNA and protein expression, Smad4 and Smad7 mRNA expressions, and epidermal growth factor and epidermal growth factor receptor (EGFR) mRNA expression in the jejunal mucosa. These findings suggest that dietary anemonin attenuates LPS-induced intestinal injury by improving mucosa restoration, alleviating intestinal inflammation and influencing TGF-\u03b21 canonical Smads and EGFR signaling pathways.<\/jats:p>","DOI":"10.1177\/1753425916648223","type":"journal-article","created":{"date-parts":[[2016,5,13]],"date-time":"2016-05-13T21:02:57Z","timestamp":1463173377000},"page":"344-352","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":30,"title":["Anemonin improves intestinal barrier restoration and influences TGF-\u03b21 and EGFR signaling pathways in LPS-challenged piglets"],"prefix":"10.1177","volume":"22","author":[{"given":"Kan","family":"Xiao","sequence":"first","affiliation":[{"name":"Animal Science College, Zhejiang University, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, 310058, 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