{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T05:26:35Z","timestamp":1785389195594,"version":"3.55.0"},"reference-count":38,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2017,9,29]],"date-time":"2017-09-29T00:00:00Z","timestamp":1506643200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Innate Immun"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:p>This study was aimed at investigating whether dietary copper\/zinc-loaded montmorillonite (Cu\/Zn-Mt) could alleviate Escherichia coli LPS-induced intestinal injury through pro- and anti-inflammatory signaling pathways (TLRs, NLRs and TGF-\u03b21) in weaned piglets. Eighteen 21-d-old pigs were randomly divided into three groups (control, LPS and LPS + Cu\/Zn-Mt). After 21\u2009d of feeding, pigs in the LPS group and LPS + Cu\/Zn-Mt group received i.p. administration of LPS, whereas pigs in the control group received saline. At 4\u2009h post-injection, jejunum samples were collected for analysis. The results indicated that, compared with the LPS group, supplemental Cu\/Zn-Mt increased transepithelial electrical resistance, the expressions of anti-inflammatory cytokines (TGF-\u03b21) in mRNA and protein levels, and decreased FD4 and the mRNA expression of pro-inflammatory cytokines (TNF-\u03b1, IL-6, IL-8 and IL-1\u03b2). The pro-inflammatory signaling pathways results demonstrated that Cu\/Zn-Mt supplementation decreased the mRNA levels of TLR4 and its downstream signals (MyD88, IRAK1, TRAF6) but had no effect on NOD1 and NOD2 signals. Cu\/Zn-Mt supplementation did not affect NF-\u03baB p65 mRNA abundance, but down-regulated its protein expression. The anti-inflammatory signaling pathways results showed supplemental Cu\/Zn-Mt also increased T\u03b2RII, Smad4 and Smad7 mRNA expressions. These findings suggested dietary Cu\/Zn-Mt attenuated LPS-induced intestinal injury by alleviating intestinal inflammation, influencing TLR4-MyD88 and TGF-\u03b21 signaling pathways in weaned pig.<\/jats:p>","DOI":"10.1177\/1753425917733033","type":"journal-article","created":{"date-parts":[[2017,9,29]],"date-time":"2017-09-29T03:41:28Z","timestamp":1506656488000},"page":"648-655","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Copper\/zinc-loaded montmorillonite influences intestinal integrity, the expression of genes associated with inflammation, TLR4\u2013MyD88 and TGF-\u03b21 signaling pathways in weaned pigs after LPS challenge"],"prefix":"10.1177","volume":"23","author":[{"given":"Lefei","family":"Jiao","sequence":"first","affiliation":[{"name":"Animal Science College, Zhejiang University, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun Chun","family":"Wang","sequence":"additional","affiliation":[{"name":"Animal Science College, Zhejiang University, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Wu","sequence":"additional","affiliation":[{"name":"Animal Science College, Zhejiang University, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Gong","sequence":"additional","affiliation":[{"name":"Animal Science College, Zhejiang University, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang Hui","family":"Lin","sequence":"additional","affiliation":[{"name":"Animal Science College, Zhejiang University, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Feng","sequence":"additional","affiliation":[{"name":"Animal Science College, Zhejiang University, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caihong","family":"Hu","sequence":"additional","affiliation":[{"name":"Animal Science College, Zhejiang University, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2017,9,29]]},"reference":[{"key":"bibr1-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00012.2006"},{"key":"bibr2-1753425917733033","doi-asserted-by":"publisher","DOI":"10.3945\/jn.112.164947"},{"key":"bibr3-1753425917733033","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2012-5796"},{"key":"bibr4-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/134.3.641"},{"key":"bibr5-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2011.12.022"},{"key":"bibr6-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1177\/1753425913499947"},{"key":"bibr7-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1016\/j.livprodsci.2003.09.011"},{"key":"bibr8-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2016.09.031"},{"key":"bibr9-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2012.10.068"},{"key":"bibr10-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-017-0156-6"},{"key":"bibr11-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1002\/cjoc.200890160"},{"key":"bibr12-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2009.06.005"},{"key":"bibr13-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.022"},{"key":"bibr14-1753425917733033","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v16.i17.2080"},{"key":"bibr15-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2002.01641.x"},{"key":"bibr16-1753425917733033","first-page":"1","volume":"9","author":"Xiao K","year":"2014","journal-title":"PLOS ONE"},{"key":"bibr17-1753425917733033","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2012-5796"},{"key":"bibr18-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1177\/1753425914536450"},{"key":"bibr19-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114510005660"},{"key":"bibr20-1753425917733033","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2005-606"},{"key":"bibr21-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1177\/1753425916648223"},{"key":"bibr22-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.5.2267-2277.2005"},{"key":"bibr23-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114509289033"},{"key":"bibr24-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1016\/j.clay.2011.01.005"},{"key":"bibr25-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1517\/17425250902865594"},{"key":"bibr26-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1097\/00005176-199205000-00007"},{"key":"bibr27-1753425917733033","doi-asserted-by":"publisher","DOI":"10.2741\/3413"},{"key":"bibr28-1753425917733033","doi-asserted-by":"publisher","DOI":"10.4141\/A05-075"},{"key":"bibr29-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1177\/1753425916632303"},{"key":"bibr30-1753425917733033","doi-asserted-by":"publisher","DOI":"10.2119\/2008-00033.Prasad"},{"key":"bibr31-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-4-50"},{"key":"bibr32-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705710"},{"key":"bibr33-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2004121070"},{"key":"bibr34-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.503946"},{"key":"bibr35-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/133.12.4077"},{"key":"bibr36-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1007\/BF01998975"},{"key":"bibr37-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1038\/ni1412"},{"key":"bibr38-1753425917733033","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.68.471"}],"container-title":["Innate Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1753425917733033","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1753425917733033","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1753425917733033","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:02:38Z","timestamp":1777460558000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1753425917733033"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,29]]},"references-count":38,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["10.1177\/1753425917733033"],"URL":"https:\/\/doi.org\/10.1177\/1753425917733033","relation":{},"ISSN":["1753-4259","1753-4267"],"issn-type":[{"value":"1753-4259","type":"print"},{"value":"1753-4267","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,29]]}}}