{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T06:46:55Z","timestamp":1790923615100,"version":"4.1.0"},"reference-count":48,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2018,4,11]],"date-time":"2018-04-11T00:00:00Z","timestamp":1523404800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"the Special Fund for Agro-scientific Research in the Public Interest","award":["201403047"],"award-info":[{"award-number":["201403047"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31472103"],"award-info":[{"award-number":["31472103"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Key R & D Program","award":["2016YFD0501210"],"award-info":[{"award-number":["2016YFD0501210"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Innate Immun"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:p>Here we investigated the influence of LPS-induced gut injury on antioxidant homeostasis, mitochondrial (mt) function and the level of mitophagy in piglets. The results showed that LPS-induced intestinal injury decreased the transepithelial electrical resistance, increased the paracellular permeability of F1TC dextran 4\u2009kDa, and decreased the expression of claudin-1, occludin and zonula occludens-1 in the jejunum compared with the control group. LPS decreased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and increased the content of malondialdehyde in the jejunum. Meanwhile, the expression of SOD-related genes ( Cu\/Zn-SOD, Mn-SOD) and GSH-Px-related genes ( GPX-1, GPX-4) declined in LPS-challenged pigs compared with the control. LPS also increased TNF-\u03b1, IL-6, IL-8 and IL-1\u03b2 mRNA expression. LPS induced mt dysfunction, as demonstrated by increased reactive oxygen species production and decreased membrane potential of intestinal mitochondria, intestinal content of mt DNA and activities of the intestinal mt respiratory chain. Furthermore, LPS induced an increase in expression of mitophagy related proteins, PTEN-induced putative kinase (PINK1) and Parkin in the intestinal mitochondria, as well as an enhancement of the ratio of light chain 3-II (LC3-II) to LC3-I content in the jejunal mucosa. These results suggested that LPS-induced intestinal injury accompanied by disrupted antioxidant homeostasis, caused mt dysfunction and triggered mitophagy.<\/jats:p>","DOI":"10.1177\/1753425918769372","type":"journal-article","created":{"date-parts":[[2018,4,12]],"date-time":"2018-04-12T01:32:34Z","timestamp":1523496754000},"page":"221-230","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":101,"title":["LPS challenge increased intestinal permeability, disrupted mitochondrial function and triggered mitophagy of piglets"],"prefix":"10.1177","volume":"24","author":[{"given":"Shuting","family":"Cao","sequence":"first","affiliation":[{"name":"Animal Science College, Zhejiang University, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianhui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Animal Science College, Zhejiang University, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ChunChun","family":"Wang","sequence":"additional","affiliation":[{"name":"Animal Science College, Zhejiang University, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Wu","sequence":"additional","affiliation":[{"name":"Animal Science College, Zhejiang University, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lefei","family":"Jiao","sequence":"additional","affiliation":[{"name":"Animal Science College, Zhejiang University, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qihua","family":"Hong","sequence":"additional","affiliation":[{"name":"Animal Science College, Zhejiang University, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caihong","family":"Hu","sequence":"additional","affiliation":[{"name":"Animal Science College, Zhejiang University, The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,4,11]]},"reference":[{"key":"bibr1-1753425918769372","volume":"9","author":"Xiao K","year":"2014","journal-title":"PloS one"},{"key":"bibr2-1753425918769372","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2012-5796"},{"key":"bibr3-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2013.12.006"},{"key":"bibr4-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-55330-6_21"},{"key":"bibr5-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.115.003046"},{"key":"bibr6-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1080\/23723556.2015.1056332"},{"key":"bibr7-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2400"},{"key":"bibr8-1753425918769372","doi-asserted-by":"publisher","DOI":"10.3945\/jn.112.164947"},{"key":"bibr9-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114515005085"},{"key":"bibr10-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-226"},{"key":"bibr11-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2014.01.010"},{"key":"bibr12-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1177\/1753425912472003"},{"key":"bibr13-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114510005660"},{"key":"bibr14-1753425918769372","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2014-8248"},{"key":"bibr15-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1210\/en.2011-1502"},{"key":"bibr16-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22993"},{"key":"bibr17-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1021\/jf1049497"},{"key":"bibr18-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1080\/1745039X.2017.1279712"},{"key":"bibr19-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.382549"},{"key":"bibr20-1753425918769372","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2013-6986"},{"key":"bibr21-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2016.01.001"},{"key":"bibr22-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1177\/1753425916648223"},{"key":"bibr23-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1177\/1753425916664124"},{"key":"bibr24-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1177\/1753425917721631"},{"key":"bibr25-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1097\/WOX.0b013e3182439613"},{"key":"bibr26-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.11.015"},{"key":"bibr27-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1007\/s10142-014-0396-x"},{"key":"bibr28-1753425918769372","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2014-8330"},{"key":"bibr29-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2008.10.001"},{"key":"bibr30-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2016.01.021"},{"key":"bibr31-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1038\/nature09973"},{"key":"bibr32-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.03.072"},{"key":"bibr33-1753425918769372","doi-asserted-by":"publisher","DOI":"10.3945\/jn.115.217661"},{"key":"bibr34-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2013.01.008"},{"key":"bibr35-1753425918769372","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21101317"},{"key":"bibr36-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2015.05.005"},{"key":"bibr37-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2012.10.011"},{"key":"bibr38-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2016.02.174"},{"key":"bibr39-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2015.06.023"},{"key":"bibr40-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2012.05.009"},{"key":"bibr41-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2014.02.008"},{"key":"bibr42-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1007\/s10068-016-0037-3"},{"key":"bibr43-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-015-1210-5"},{"key":"bibr44-1753425918769372","first-page":"126","volume":"83","author":"Hirota Y","year":"2011","journal-title":"Seikagaku"},{"key":"bibr45-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1002\/path.4774"},{"key":"bibr46-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2015.01.002"},{"key":"bibr47-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1038\/nature14893"},{"key":"bibr48-1753425918769372","doi-asserted-by":"publisher","DOI":"10.1039\/C4RA13557A"}],"container-title":["Innate Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1753425918769372","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1753425918769372","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1753425918769372","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:02:42Z","timestamp":1777460562000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1753425918769372"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4,11]]},"references-count":48,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1177\/1753425918769372"],"URL":"https:\/\/doi.org\/10.1177\/1753425918769372","relation":{},"ISSN":["1753-4259","1753-4267"],"issn-type":[{"value":"1753-4259","type":"print"},{"value":"1753-4267","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4,11]]}}}