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After LPS treatment, the mRNA expression of IL-1\u03b2, IL-6, and TNF-\u03b1 reached maximal levels at 1 h, 2 h, and 1 h, respectively ( P\u2009&lt;\u20090.05), and mRNA expression of TLR4, NODs, muscle-specific ring finger 1, and muscle atrophy F-box peaked at 12\u2009h ( P\u2009&lt;\u20090.05). Moreover, the expression of miR-122, miR-135a, and miR-370 reduced at 1 h, 1 h, and 2 h, respectively ( P\u2009&lt;\u20090.05), and miR-34a, miR-224, miR-132, and miR-145 reached maximum expression levels at 1 h, 1 h, 2 h, and 4 h, respectively ( P\u2009&lt;\u20090.05). These results suggested that mRNA expression of pro-inflammatory cytokines was elevated in the early stage, mRNA expression of genes related to TLR4 and NODs signaling pathways and protein degradation increased in the later phase, and the expression of microRNA related to muscle inflammation and protein degradation changed in the early stage after LPS injection.<\/jats:p>","DOI":"10.1177\/1753425920971032","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T17:20:30Z","timestamp":1606238430000},"page":"23-30","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Kinetics of changes in gene and microRNA expression related with muscle inflammation and protein degradation following LPS-challenge in weaned piglets"],"prefix":"10.1177","volume":"27","author":[{"given":"Ping","family":"Kang","sequence":"first","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and 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