{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T14:23:06Z","timestamp":1777731786805,"version":"3.51.4"},"reference-count":28,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2020,11,26]],"date-time":"2020-11-26T00:00:00Z","timestamp":1606348800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Corps Major Scientific and Technological Projects","award":["No. 2017AA003"],"award-info":[{"award-number":["No. 2017AA003"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No. U1803236"],"award-info":[{"award-number":["No. U1803236"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Natural Key Research and Development Plan","award":["No. 2017YFD0500304"],"award-info":[{"award-number":["No. 2017YFD0500304"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Innate Immun"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>Brucella is a genus of Gram-negative intracellular pathogens that cause animal and human diseases. Brucella survival and replication inside immune cells is critical for the establishment of chronic infections. Protein modifications by small ubiquitin-related modifier proteins and the NF-\u03baB pathway are involved in many cellular activities, playing major roles in regulating protein function that is essential for pathogenic bacteria during infection. However, the relationship between them in the intracellular survival of Brucella is still largely unknown. We demonstrated that Brucella abortus 2308 infection can activate the expression of small ubiquitin-related modifier-2 proteins in a time-dependent manner. We found the production of Th1 cytokines (IFN-\u03b3 and TNF-\u03b1) and the transcription of NF-\u03baB\/p65 were promoted by overexpression and inhibited by interference of small ubiquitin-related modifier-2. In addition, we showed that small ubiquitin-related modifier-2 can inhibit intracellular survival of Brucella abortus 2308 by regulating activation of the NF-\u03baB pathway. Taken together, this work shows that small ubiquitin-related modifier-2 modification of NF-\u03baB2\/p65 is essential for the survival of Brucella abortus 2308 inside macrophages. This work may help to unravel the pathogenic mechanisms of Brucella infections.<\/jats:p>","DOI":"10.1177\/1753425920972171","type":"journal-article","created":{"date-parts":[[2020,11,26]],"date-time":"2020-11-26T08:36:00Z","timestamp":1606379760000},"page":"81-88","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Small ubiquitin-related modifier 2 affects the intracellular survival of\n                    <i>Brucella abortus<\/i>\n                    2308 by regulating activation of the NF-\u03baB pathway"],"prefix":"10.1177","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5320-4953","authenticated-orcid":false,"given":"Yueli","family":"Wang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, 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