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Further, any new antibiotics are likely to have a short shelf life. Ab-based interventions offer alternatives that are not likely to be circumvented by the widely prevalent antibiotic resistance genes. Bovine colostrum (BC)\u2014the first milk after parturition, rich in nutrients and immune components\u2014promotes gut integrity and modulates the gut microbiome. We developed a hyperimmune BC (HBC) enriched in Abs to a highly conserved LOS core region of Gram-negative bacteria by immunizing pregnant cows with a vaccine comprised of detoxified LOS from Escherichia coli O111 Rc (J5) mutant non-covalently complexed to group B meningococcal outer membrane protein (J5dLOS\/OMP). This vaccine generated robust levels of anti-J5 LOS Ab in the colostrum. When given orally to neutropenic rats challenged orally with Pseudomonas aeruginosa, administration of HBC improved survival compared to non-immune rats, while both BC preparations improved survival compared to PBS controls. Elevated circulating endotoxin levels correlated with mortality. HBC and to a lesser extent non-immune BC reduced bacterial burden from the liver, lung, and spleen. We conclude that HBC and to a lesser extent BC may be effective supplements that improve outcome from lethal gut-derived disseminated infection and may reduce transmission of Gram-negative bacilli from the gastrointestinal tract.<\/jats:p>","DOI":"10.1177\/17534259211007538","type":"journal-article","created":{"date-parts":[[2021,4,16]],"date-time":"2021-04-16T03:47:07Z","timestamp":1618544827000},"page":"266-274","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["A pilot study of an anti-endotoxin Ig-enriched bovine colostrum to prevent experimental sepsis"],"prefix":"10.1177","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4362-9936","authenticated-orcid":false,"given":"Alan S","family":"Cross","sequence":"first","affiliation":[{"name":"Center for Vaccine Development and Global Health, University of Maryland School of Medicine, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven M","family":"Opal","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Rhode Island Hospital, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John E","family":"Palardy","sequence":"additional","affiliation":[{"name":"Infectious Disease Division, Memorial Hospital of RI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Surekha","family":"Shridhar","sequence":"additional","affiliation":[{"name":"Center for Vaccine Development and Global Health, University of Maryland School of Medicine, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott M","family":"Baliban","sequence":"additional","affiliation":[{"name":"Center for Vaccine Development and Global Health, University of Maryland School of Medicine, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alison J","family":"Scott","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis, University of Maryland School of Dentistry, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdullah B","family":"Chahin","sequence":"additional","affiliation":[{"name":"Critical Care Division, Kent County Hospital, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert K","family":"Ernst","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis, University of Maryland School of Dentistry, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,4,16]]},"reference":[{"key":"bibr1-17534259211007538","volume-title":"Antibiotic resistance threats in the United States, 2019","author":"CDC","year":"2019"},{"key":"bibr2-17534259211007538","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2014-8428"},{"key":"bibr3-17534259211007538","doi-asserted-by":"publisher","DOI":"10.1126\/science.1248056"},{"key":"bibr4-17534259211007538","doi-asserted-by":"publisher","DOI":"10.1038\/nature10388"},{"key":"bibr5-17534259211007538","first-page":"e00543","volume":"8","author":"Yin W","year":"2017","journal-title":"mBio"},{"key":"bibr6-17534259211007538","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034953"},{"key":"bibr7-17534259211007538","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-016-1549-1"},{"key":"bibr8-17534259211007538","volume-title":"Antimicrobial resistance: global report on surveillance","author":"World Health Organization","year":"2014"},{"key":"bibr9-17534259211007538","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.16.020916"},{"key":"bibr10-17534259211007538","unstructured":"O\u2019Neill J. 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