{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T17:31:24Z","timestamp":1788543084957,"version":"build-2803163510"},"reference-count":45,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T00:00:00Z","timestamp":1623974400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81500091"],"award-info":[{"award-number":["81500091"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Scientific Research Project of Education Department of Hunan Province","award":["18C0425"],"award-info":[{"award-number":["18C0425"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Innate Immun"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:p>TLRs recognizing PAMPS play a role in local immunity and participate in implant-associated loosening. TLR-mediated signaling is primarily regulated by IL-1 receptor associated kinase-M (IRAK-M) negatively and IRAK-4 positively. Our previous studies have proved that wear particles promote endotoxin tolerance in macrophages by inducing IRAK-M. However, whether IRAK-4 is involved in inflammatory osteolysis of wear particles basically, and the specific mechanism of IRAK-4 around loosened hip implants, is still unclear. IRAK-4 was studied in the interface membranes from patients in vivo and in particle-stimulated macrophages to clarify its role. Also, IL-1\u03b2 and TNF-\u03b1 levels were measured after particle and LPS stimulation in macrophages with or without IRAK-4 silenced by siRNA. Our results showed that the interface membranes around aseptic and septic loosened prosthesis expressed more IRAK-4 compared with membranes from osteoarthritic patients. IRAK-4 in macrophages increased upon particle and LPS stimulation. In the former, IL-1\u03b2 and TNF-\u03b1 levels were lower compared with those of LPS stimulation, and IRAK-4 siRNA could suppress production of pro-inflammatory cytokines. These findings suggest that besides IRAK-M, IRAK-4 also plays an important role in the local inflammatory reaction and contributes to prosthesis loosening.<\/jats:p>","DOI":"10.1177\/17534259211018740","type":"journal-article","created":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T04:51:02Z","timestamp":1623991862000},"page":"470-482","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["IRAK-4 in macrophages contributes to inflammatory osteolysis of wear particles around loosened hip implants"],"prefix":"10.1177","volume":"27","author":[{"given":"Yang-chun","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Orthopedics, People\u2019s Hospital of Shenzhen Baoan District, China"},{"name":"Department of Orthopedics, The First Affiliated Hospital of University of South China, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-hong","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Hematology, Huazhong University of Science and Technology Union Shenzhen Hospital, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao-jie","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, People\u2019s Hospital of Shenzhen Baoan District, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1032-2834","authenticated-orcid":false,"given":"Guo-liang","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, The First Affiliated Hospital of University of South China, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,6,18]]},"reference":[{"key":"bibr1-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2001.16.10.1780"},{"key":"bibr2-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2001.16.1.79"},{"key":"bibr3-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-014-0099-4"},{"key":"bibr4-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34375"},{"key":"bibr5-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.33258"},{"key":"bibr6-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1086\/653003"},{"key":"bibr7-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20038"},{"key":"bibr8-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01762-07"},{"key":"bibr9-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa061588"},{"key":"bibr10-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1097\/01.blo.0000224050.94248.38"},{"key":"bibr11-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.30030"},{"key":"bibr12-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1159\/000373996"},{"key":"bibr13-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.136895"},{"key":"bibr14-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.30992"},{"key":"bibr15-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2001.16.11.2082"},{"key":"bibr16-17534259211018740","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.080390"},{"key":"bibr17-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.30512"},{"key":"bibr18-17534259211018740","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.1002\/jbm.a.32495","volume":"92","author":"Pajarinen J","year":"2010","journal-title":"J Biomed Mater Res A"},{"key":"bibr19-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.32674"},{"key":"bibr20-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2007.12.012"},{"key":"bibr21-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1126\/science.1124256"},{"key":"bibr22-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.31235"},{"key":"bibr23-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.31235"},{"key":"bibr24-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100170603"},{"key":"bibr25-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1615\/JLongTermEffMedImplants.2014010562"},{"key":"bibr26-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34599"},{"key":"bibr27-17534259211018740","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1002\/jbm.a.32474","volume":"92","author":"Hao HN","year":"2010","journal-title":"J Biomed Mater Res A"},{"key":"bibr28-17534259211018740","doi-asserted-by":"publisher","DOI":"10.5435\/00124635-200800001-00012"},{"key":"bibr29-17534259211018740","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.30150"},{"key":"bibr30-17534259211018740","doi-asserted-by":"crossref","unstructured":"Nelson CL, McLaren AC, McLaren SG, et\u00a0al. 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