{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T11:00:03Z","timestamp":1780570803418,"version":"3.54.1"},"reference-count":32,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100018571","name":"Guangxi Science and Technology Base and Talent Special Project","doi-asserted-by":"crossref","award":["Guike AD24999032"],"award-info":[{"award-number":["Guike AD24999032"]}],"id":[{"id":"10.13039\/501100018571","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Promotion Project of Scientific Research Ability of Young and Middle-aged Teachers in Guangxi Universities in 2023","award":["2023KY0539"],"award-info":[{"award-number":["2023KY0539"]}]},{"name":"Guangxi Natural Science Foundation","award":["2022GXNSFAA035626"],"award-info":[{"award-number":["2022GXNSFAA035626"]}]},{"DOI":"10.13039\/501100017691","name":"Guangxi Key Research and Development Plan","doi-asserted-by":"crossref","award":["Guike AB24010096"],"award-info":[{"award-number":["Guike AB24010096"]}],"id":[{"id":"10.13039\/501100017691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Innate Immun"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:p>Bronchial cell pyroptosis and IL-17 respectively contribute- to the pathogenesis of steroid-insensitive asthma. In this study, we aim to explore the relationship between bronchial cell pyroptosis and Th17 in airway inflammation of steroid-insensitive asthma. The steroid-insensitive asthma model of mice was induced by toluene diisocyanate (TDI), which was also intraperitoneally injected with NLRP3 (NOD-, LRR- and pyrin domain-containing protein 3) inhibitor MCC950. The bronchial epithelial cell pyroptosis was identified in morphology by transmission electron microscope. Protein expressions of pyroptosis cytokines (pro-Caspase-1, Caspase-1 p20, pro-GSDMD, cleaved-GSDMD and HMGB1), IL-17A, IL-17F and phosphorylated STAT3 (p-STAT3) in lung tissues were assessed by western blotting. Th17 in lung tissues was measured by flow cytometry. IL-17A\u2009+\u2009and p-STAT3\u2009+\u2009cells in airway were identified by immunohistochemistry. In steroid-insensitive asthma mice, bronchial epithelial cell pyroptosis was confirmed in morphology using transmission electron microscope. Compared with controls, the protein expressions of Caspase-1 p20, cleaved-GSDMD and HMGB1 in lung tissues were increased in mice with steroid-insensitive asthma, which could be attenuated by MCC950. Th17 cells precentage and proteins expressions of p-STAT3, IL-17A and IL-17F were also increased in lung of steroid-insensitive asthmatic mice, which were also attenuated by MCC950. Similarly, the counts of IL-17A\u2009+\u2009cell and p-STAT3\u2009+\u2009cell were more in airway of steroid-insensitive asthmatic mice than controls, and was attenuated by MCC950. In conclusion, bronchial epithelial cell pyroptosis could promote Th17 inflammation in airway of steroid-insensitive asthma mouse, which will provide further understanding on the interaction between innate immunity and acquired immunity in the pathogenesis of steroid-insensitive asthma.<\/jats:p>","DOI":"10.1177\/17534259251372592","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T11:20:35Z","timestamp":1756380035000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Bronchial pyroptosis promotes Th17 inflammation in steroid-insensitive asthma mouse"],"prefix":"10.1177","volume":"31","author":[{"given":"Yun","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, the Second Affiliated Hospital of Guilin Medical University, Guilin, China"},{"name":"Guangxi Key Laboratory of Metabolic Reprogramming and Intelligent 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