{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T20:10:06Z","timestamp":1774642206394,"version":"3.50.1"},"reference-count":32,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100022110","name":"BioM\u00e9rieux","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100022110","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Hong Kong Lung Foundation"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ther Adv Respir Dis"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Acute exacerbations of bronchiectasis (AEB) are frequently caused by bacterial and\/or viral infections. Rapid multiplex polymerase chain reaction (PCR) panels in respiratory specimens have significantly advanced microbial evaluation in patients with pneumonia. However, their clinical utility in patients with AEB remains unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives:<\/jats:title>\n                    <jats:p>To investigate the microbial characteristics of AEB using FilmArray Pneumonia plus Panel (FAPP) and explore its clinical impact in a real-world setting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design:<\/jats:title>\n                    <jats:p>A cross-sectional study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Spontaneous sputum samples of patients hospitalized for AEB were tested using FAPP in addition to standard-of-care testing. Microbial characteristics of AEB and the clinical impact of FAPP were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Among 83 patients, FAPP detected \u2a7e1 bacterial pathogen(s) in 68 samples (81.9%), identifying 101 bacteria, with high abundance (10\n                      <jats:sup>6<\/jats:sup>\n                      to \u2a7e10\n                      <jats:sup>7<\/jats:sup>\n                      \u2009copies\/ml) observed in 48 patients (57.8%). The most commonly detected bacteria were\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">Pa<\/jats:italic>\n                      ) (37\/83, 44.6%),\n                      <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                      (21\/83, 25.3%), and\n                      <jats:italic toggle=\"yes\">Haemophilus influenzae<\/jats:italic>\n                      (13\/83, 15.7%). Respiratory viruses were identified in 21 patients (25.3), with\n                      <jats:italic toggle=\"yes\">Influenza A<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Respiratory syncytial virus<\/jats:italic>\n                      being the most common. Culture detected bacteria in significantly fewer samples (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      \u2009=\u200925 [30.1%],\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.001). FAPP demonstrated 100% positive percent agreement and negative predictive value for all cultured bacteria, except for\n                      <jats:italic toggle=\"yes\">Corynebacterium striatum<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      \u2009=\u20092), which was not included in the panel. FAPP shortened the time to bacterial results (mean: 10.8\u2009h vs 70.8\u2009h by culture,\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.001), and led to antimicrobial changes in 25 patients (30.1%) before the culture results were available. In multivariate analysis, chronic\n                      <jats:italic toggle=\"yes\">Pa<\/jats:italic>\n                      infection (odds ratio (OR) 14.71), underweight status (OR 5.84), and cystic bronchiectasis (OR 5.26) were independent predictors of\n                      <jats:italic toggle=\"yes\">Pa<\/jats:italic>\n                      detection by FAPP.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The sputum multiplex PCR panel (FAPP) enables rapid identification of bacterial and viral pathogens in AEB, supporting early antimicrobial decision-making. Our findings highlight the potential utility of sputum multiplex PCR panels in improving the management of bronchiectasis exacerbations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/17534666251341751","type":"journal-article","created":{"date-parts":[[2025,6,21]],"date-time":"2025-06-21T11:16:58Z","timestamp":1750504618000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Rapid microbial evaluation of acute exacerbations of bronchiectasis using FilmArray Pneumonia plus Panel in a real-world setting"],"prefix":"10.1177","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3336-2414","authenticated-orcid":false,"given":"Charles","family":"Wong","sequence":"first","affiliation":[{"name":"Department of Medicine, Pamela Youde Nethersole Eastern Hospital, 3 Lok Man Road, Chai Wan, Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun Wai","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pamela Youde Nethersole Eastern Hospital, Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hei Shun","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pamela Youde Nethersole Eastern Hospital, Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pui Hing","family":"Chiu","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pamela Youde Nethersole Eastern Hospital, Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flora Pui Ling","family":"Miu","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pamela Youde Nethersole Eastern Hospital, Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiu Wing","family":"Lam","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Pamela Youde Nethersole Eastern Hospital, Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Loretta Yin Chun","family":"Yam","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pamela Youde Nethersole Eastern Hospital, Hong Kong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,6,21]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-018-0042-3"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.21037\/atm-22-3437"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201711-2202OC"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1183\/16000617.0108-2018"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1183\/16000617.0085-2024"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2018-212463"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(24)00170-8"},{"key":"e_1_3_4_9_2","volume-title":"Reducing bacterial resistance with IMPACT","author":"Ho PL","year":"2017","unstructured":"Ho PL, Wu TC, Chao DV, et al. 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