{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:37:28Z","timestamp":1753882648400,"version":"3.41.2"},"reference-count":34,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014538","name":"LPDP","doi-asserted-by":"crossref","award":["20160821048782"],"award-info":[{"award-number":["20160821048782"]}],"id":[{"id":"10.13039\/501100014538","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Therapeutic Advances in Urology"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec><jats:title>Background:<\/jats:title><jats:p> Treatment of urethral trauma is currently done after urethral stricture occurs. Stricture therapy after occurrence gives unsatisfactory success rates. Several genes, such as transforming growth factor beta 1 (TGF-\u03b21), matrix metalloproteinase\u20091 (MMP-1), connective tissue growth factor (CTGF), and plasminogen activator inhibitor 1 (PAI-1), have a proven role in urethral stricture development. The purpose of this study was to assess the effect docetaxel and\/or captopril on the RNA expression of those genes. <\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p> The subjects of this research were 26 male New Zealand rabbits aged 230\u2009\u00b1\u200920\u2009days weighing 4\u20135\u2009kg that underwent urethral rupture by endoscopic resection under anesthetized conditions. Subjects were divided into five groups; control, stricture, captopril (captopril 0.05\u2009mg\/rabbit\/day), docetaxel (docetaxel 0.1\u2009mg\/rabbit\/day), and docetaxel-captopril (docetaxel 0.1\u2009mg\/rabbit\/day and captopril 0.05\u2009mg\/rabbit\/day). Each group consisted of 4\u20136 rabbits. Each rabbit received a water-soluble transurethral gel containing drug according to its group for 28\u2009days. After the treatment period, rabbits were sacrificed with 200\u2009mg Pentothal, and the corpus spongiosum was then prepared for real-time PCR examination. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p> TGF-\u03b21 RNA expression in the stricture group was statistically different from that in the control, docetaxel and docetaxel-captopril groups ( p\u2009=\u20090.016; p\u2009=\u20090.016; p\u2009=\u20090.004). The stricture group did not exhibit any statistical difference from the captopril group ( p\u2009=\u20090.190). The control group did not show any statistically difference from the captopril, docetaxel, and docetaxel-captopril groups ( p\u2009=\u20090.114; p\u2009=\u20090.190; p\u2009=\u20091.000). Docetaxel-captopril suppresses expression of TGF-\u03b21 RNA most significantly. MMP-1 RNA expression showed no significant differences among groups ( p\u2009=\u20090.827). The docetaxel group and stricture group pair was most significant ( p\u2009=\u20090.247), compared with other pairs of stricture groups in MMP-1 RNA expression. CTGF RNA expression in the stricture group was statistically different from that of control, captopril, docetaxel, and docetaxel-captopril groups ( p\u2009=\u20090.003; p\u2009=\u20090.019; p\u2009=\u20090.005; p\u2009=\u20090.005). The control group did not exhibit any statistically difference from the captopril, docetaxel, and docetaxel-captopril groups ( p\u2009=\u20090.408; p\u2009=\u20090.709; p\u2009=\u20090.695). There was no statistical difference among treatment groups. Docetaxel and docetaxel-captopril groups suppress the most significant expression of CTGF RNA expression. PAI-1 RNA expression in the stricture group differed statistically significantly from the control and docetaxel groups ( p\u2009=\u20090.044; p\u2009=\u20090.016). The stricture group did not show any statistically significant difference from the captopril and docetaxel-captopril groups ( p\u2009=\u20090.763; p\u2009=\u20090.086). The control group did not exhibit any statistical difference with any of the treatment groups ( p\u2009=\u20090.101; p\u2009=\u20090.637; p\u2009=\u20090.669). <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p> Docetaxel-captopril gel proved to be able to inhibit RNA expression of TGF-\u03b21 and CTGF significantly. Captopril gel proved to be able to inhibit RNA expression of CTGF significantly. Docetaxel gel proved to be able to inhibit RNA expression of TGF-\u03b21, CTGF, and PAI-1 significantly. There were no differences in MMP-1 expression among all study groups. Longer follow up after therapy discontinuation and greater sample size is needed to determine the therapeutic effect. <\/jats:p><\/jats:sec>","DOI":"10.1177\/1756287220927994","type":"journal-article","created":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T11:10:47Z","timestamp":1603278647000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["The effects of docetaxel and\/or captopril in expression of TGF-\u03b21, MMP-1, CTGF, and PAI-1 as markers of anterior urethral stricture in an animal model"],"prefix":"10.1177","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0704-1369","authenticated-orcid":false,"given":"Wikan","family":"Kurniawan","sequence":"first","affiliation":[{"name":"Department of Urology, Academic Hospital, Universitas Gadjah Mada, North Ring Road, Kronggahan, Trihanggo, Gamping, Sleman, Yogyakarta, 55291 Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marsetyawan Heparis Nur Ekandaru","family":"Soesatyo","sequence":"additional","affiliation":[{"name":"Department of Histology and Cellular Biology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teguh","family":"Aryandono","sequence":"additional","affiliation":[{"name":"Department of Histology and Cellular Biology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia"},{"name":"Department of Surgical Oncology, Sardjito General Hospital, Yogyakarta, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,10,21]]},"reference":[{"key":"bibr1-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1016\/j.eursup.2015.10.002"},{"key":"bibr2-1756287220927994","volume":"116","author":"Hua AX","year":"2018","journal-title":"et al"},{"key":"bibr3-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2016.02.034"},{"key":"bibr4-1756287220927994","first-page":"304","volume":"32","author":"Mangera A","year":"2014","journal-title":"Surg"},{"key":"bibr5-1756287220927994","first-page":"313","volume":"35","author":"Watkin N","year":"2017","journal-title":"Surg (United Kingdom)"},{"key":"bibr6-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajur.2017.12.005"},{"key":"bibr7-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2017.07.022"},{"key":"bibr8-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1016\/j.eursup.2015.10.001"},{"key":"bibr9-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1016\/j.afju.2016.01.003"},{"key":"bibr10-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2014.03.002"},{"key":"bibr11-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1177\/1535370218761628"},{"key":"bibr12-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2009.12.001"},{"key":"bibr13-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpurol.2017.08.013"},{"key":"bibr14-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0112097"},{"key":"bibr15-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.2007.01693.x"},{"key":"bibr16-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1007\/s00240-003-0333-2"},{"key":"bibr17-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2012.04.025"},{"key":"bibr18-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1016\/j.spinee.2004.05.251"},{"key":"bibr19-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2007.10.024"},{"key":"bibr20-1756287220927994","unstructured":"National Center for Biotechnology Information, U.S. National Library of Medicine. Primer-BLAST detailed primer reports. Detailed primer reports CTGF. Bethesda MD: National Center for Biotechnology Information, U.S. National Library of Medicine, https:\/\/www.ncbi.nlm.nih.gov\/tools\/primer-blast\/primertool.cgi?ctg_time=1594353744&job_key=ysAV1RUYGLA_jh2LEOs5uWrwKItH4zOWRg&CheckStatus=Check (accessed 21 June 2018)."},{"key":"bibr21-1756287220927994","unstructured":"National Center for Biotechnology Information, U.S. National Library of Medicine. Primer-BLAST Detailed primer reports. Detailed primer reports. Bethesda MD: National Center for Biotechnology Information, U.S. National Library of Medicine, https:\/\/www.ncbi.nlm.nih.gov\/tools\/primer-blast\/primertool.cgi?ctg_time=1594353165&job_key=urBlpSTIKWAOWrlftD-dbc4kjF_jN5dC4g&CheckStatus=Check (accessed 21 June 2018)."},{"key":"bibr22-1756287220927994","unstructured":"National Center for Biotechnology Information, U.S. National Library of Medicine. Primer-BLAST Detailed primer reports. Detailed primer reports GAPDH. Bethesda MD: National Center for Biotechnology Information, U.S. National Library of Medicine, https:\/\/www.ncbi.nlm.nih.gov\/tools\/primer-blast\/primertool.cgi?ctg_time=1594353478&job_key=joRRkRDIHWA6Wo1fgD-pbfokuF_XN6NC1 (accessed 21 June 2018)."},{"key":"bibr23-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1177\/1753425913488012"},{"key":"bibr24-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1177\/1535370213509562"},{"key":"bibr25-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1177\/1945892418770291"},{"key":"bibr26-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(99)00021-0"},{"key":"bibr27-1756287220927994","first-page":"87","volume":"139","author":"Stern-straeter J","year":"2017","journal-title":"Transl Basic Res Oral Present"},{"key":"bibr28-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-2982.2006.01269.x"},{"key":"bibr29-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509339487"},{"key":"bibr30-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1177\/172460089901400406"},{"key":"bibr31-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1177\/1947603511435273"},{"key":"bibr32-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1177\/039463201102400305"},{"key":"bibr33-1756287220927994","first-page":"7","volume":"7","author":"Sriram S","year":"2016","journal-title":"Bone Tissue Regen Insights"},{"key":"bibr34-1756287220927994","doi-asserted-by":"publisher","DOI":"10.1177\/43.9.7642963"}],"container-title":["Therapeutic Advances in Urology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1756287220927994","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1756287220927994","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1756287220927994","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,2]],"date-time":"2025-03-02T03:23:11Z","timestamp":1740885791000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1756287220927994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":34,"alternative-id":["10.1177\/1756287220927994"],"URL":"https:\/\/doi.org\/10.1177\/1756287220927994","relation":{},"ISSN":["1756-2872","1756-2880"],"issn-type":[{"type":"print","value":"1756-2872"},{"type":"electronic","value":"1756-2880"}],"subject":[],"published":{"date-parts":[[2020,1]]},"article-number":"1756287220927994"}}