{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:54:50Z","timestamp":1790747690961,"version":"4.1.0"},"reference-count":16,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Infection Prevention"],"published-print":{"date-parts":[[2026,9]]},"abstract":"\n Background<\/jats:title>\n Healthcare workers in critical care settings are at increased risk of acquiring respiratory infections, particularly during aerosol-generating procedures (AGPs). Portable negative-pressure isolation devices have been proposed to fix airborne infection in isolation rooms.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n To evaluate whether an isolated negative-pressure device reduces bacterial and fungal aerosol dispersion under controlled laboratory conditions.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n This laboratory-based controlled experimental observational study assessed aerosol containment across three techniques: (1) no cover, (2) plastic sheet canopy barrier, and (3) a portable negative-pressure isolation system. Standardized 5\u00a0mL suspensions of Staphylococcus epidermidis and Aspergillus flavus were nebulized on three separate days for each technique. Nutrient agar plates were placed and colony-forming units (CFU) were manually counted. The primary outcome was the mean CFU per technique per day. One-way ANOVA with post-hoc Welch\u2019s\n t<\/jats:italic>\n -tests (Bonferroni-corrected) was used for statistical comparison.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n A total of 36 bacterial and fungal plates were analysed. For bacterial aerosols, mean CFU was highest with no cover (535.6 \u00b1 5.38), lower with plastic sheet canopy barrier (429.5 \u00b1 5.41), and lowest with the negative-pressure system (217.5 \u00b1 17.5). Differences were statistically significant (ANOVA\n p<\/jats:italic>\n = 9.8 \u00d7 10\n \u22128<\/jats:sup>\n ; \u03b7\n 2<\/jats:sup>\n = 0.995). Fungal CFU demonstrated a similar pattern with significantly lower CFU for Technique 3 compared with both other techniques (\n p<\/jats:italic>\n < .001).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n The negative-pressure isolation device significantly reduced airborne dispersion of bacterial and fungal aerosols compared with plastic sheet canopy barrier methods. These findings support the use of portable negative-pressure systems to enhance aerosol containment during AGPs. Further clinical validation is recommended.<\/jats:p>\n <\/jats:sec>","DOI":"10.1177\/17571774261477869","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T05:45:41Z","timestamp":1787895941000},"page":"251-260","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Can isolated negative-pressure devices reduce transmission of respiratory infection in critical care units: A laboratory-based controlled experimental observational study"],"prefix":"10.1177","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7935-8598","authenticated-orcid":false,"given":"Sunit Kumar","family":"Gupta","sequence":"first","affiliation":[{"name":"AIIMS Mangalagiri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidhima","family":"Aggarwal","sequence":"additional","affiliation":[{"name":"AIIMS Mangalagiri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6539-889X","authenticated-orcid":false,"given":"Nisha M.","family":"P","sequence":"additional","affiliation":[{"name":"AIIMS Mangalagiri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7590-1047","authenticated-orcid":false,"given":"Vamshidhar","family":"Chamala","sequence":"additional","affiliation":[{"name":"AIIMS Mangalagiri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samarjit","family":"Dey","sequence":"additional","affiliation":[{"name":"All India Institute of Medical Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eeshwar M.","family":"V","sequence":"additional","affiliation":[{"name":"AIIMS Mangalagiri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7853-5959","authenticated-orcid":false,"given":"Sumit","family":"Bansal","sequence":"additional","affiliation":[{"name":"AIIMS Rajkot"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mukesh","family":"Tripathi","sequence":"additional","affiliation":[{"name":"ANIIMS"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jitendra","family":"Chawla","sequence":"additional","affiliation":[{"name":"All India Institute of Medical Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"key":"e_1_3_6_2_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01017-2020"},{"issue":"3","key":"e_1_3_6_3_1","first-page":"165","article-title":"The airborne transmission of infection in hospital buildings: fact or fiction?","volume":"53","author":"Beggs CB","year":"2003","unstructured":"Beggs CB (2003) The airborne transmission of infection in hospital buildings: fact or fiction? Journal of Hospital Infection 53(3): 165\u2013172. https:\/\/doi.org\/10.1053\/jhin.2003.1343","journal-title":"Journal of Hospital Infection"},{"issue":"5","key":"e_1_3_6_4_1","first-page":"524","article-title":"An aerosol containment and filtration tent for intubation during the COVID-19 pandemic","volume":"28","author":"Brant-Zawadzki GM","year":"2021","unstructured":"Brant-Zawadzki GM, Ockerse P, Brunson JR, et al. (2021) An aerosol containment and filtration tent for intubation during the COVID-19 pandemic. 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