{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T11:51:31Z","timestamp":1791978691356,"version":"4.4.0"},"reference-count":21,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Infection Prevention"],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Methicillin-resistant\n                      <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                      (MRSA) remains a major cause of healthcare-associated infections worldwide. Healthcare workers (HCWs) colonized with MRSA may act as reservoirs for transmission, contributing to hospital- and community-acquired infections. Early detection of MRSA carriage is essential for effective infection-prevention and control.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>\n                      This cross-sectional study was conducted at a tertiary care hospital. A total of 407 HCWs were screened for MRSA colonization using anterior nasal swab specimens.\n                      <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                      was identified using standard microbiological techniques. Methicillin resistance was determined phenotypically by the cefoxitin disc diffusion method according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Genotypic confirmation was performed using real-time polymerase chain reaction (RT-PCR) to detect the\n                      <jats:italic toggle=\"yes\">mecA<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">mecC<\/jats:italic>\n                      genes. MRSA carriers received 2% intranasal mupirocin followed by repeat screening.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among 407 HCWs screened, 96\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      isolates were recovered, of which 80 (83.3%) were phenotypically confirmed as MRSA. The overall MRSA nasal carriage rate was 19.65%, with the highest prevalence among interns (40.62%). Male gender was significantly associated with MRSA carriage (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      &lt; 0.05). Among the 80 MRSA isolates,\n                      <jats:italic toggle=\"yes\">mecA<\/jats:italic>\n                      was detected in 55 (68.75%), including 30 (37.5%) co-harbouring\n                      <jats:italic toggle=\"yes\">mecC<\/jats:italic>\n                      . No isolate carried\n                      <jats:italic toggle=\"yes\">mecC<\/jats:italic>\n                      alone, while 25 (31.25%) lacked both genes. All carriers became culture-negative after decolonization.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>A substantial proportion of HCWs were colonized with MRSA, highlighting their role in nosocomial transmission. Combined phenotypic and genotypic methods improved MRSA detection. Routine surveillance, strict infection-control measures, and targeted decolonization are essential to reduce MRSA transmission.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/17571774261497956","type":"journal-article","created":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T15:41:52Z","timestamp":1791733312000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular detection of\n                    <i>mecA<\/i>\n                    and\n                    <i>mecC-<\/i>\n                    Mediated MRSA nasal carriage among healthcare workers in a tertiary care hospital"],"prefix":"10.1177","author":[{"given":"Ajay","family":"Yadav","sequence":"first","affiliation":[{"name":"Swami Vivekanand Subharti University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8143-3083","authenticated-orcid":false,"given":"Kalpana","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Swami Vivekanand Subharti University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8660-5550","authenticated-orcid":false,"given":"Ravi Pratap","family":"Singh","sequence":"additional","affiliation":[{"name":"Swami Vivekanand Subharti University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7645-1674","authenticated-orcid":false,"given":"Anita","family":"Pandey","sequence":"additional","affiliation":[{"name":"Swami Vivekanand Subharti University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,10,11]]},"reference":[{"key":"e_1_3_9_2_1","doi-asserted-by":"publisher","DOI":"10.22207\/JPAM.19.1.35"},{"key":"e_1_3_9_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/jfmpc.jfmpc_387_25"},{"key":"e_1_3_9_4_1","unstructured":"Centers for Disease Control and Prevention (CDC) (2024) Strategies to prevent hospital-onset Staphylococcus aureus bloodstream infections in acute care facilities. Available at. https:\/\/www.cdc.gov\/staphylococcus-aureus\/hcp\/prevent-in-acute-care-facilities\/ (accessed 29 March 2026)."},{"key":"e_1_3_9_5_1","unstructured":"Centers for Disease Control and Prevention (CDC) (2025) Clinical overview of methicillin-resistant Staphylococcus aureus (MRSA) in healthcare settings. Available at. https:\/\/www.cdc.gov\/mrsa\/hcp\/clinical-overview\/index.html (accessed 29 March 2026)."},{"key":"e_1_3_9_6_1","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing","author":"Clinical and Laboratory Standards Institute (CLSI)","year":"2024","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2024) Performance Standards for Antimicrobial Susceptibility Testing. 34th edition. Clinical and Laboratory Standards Institute. CLSI supplement M100.","edition":"34"},{"key":"e_1_3_9_7_1","volume-title":"Mackie & Mccartney Practical Medical Microbiology","author":"Collee JG","year":"1996","unstructured":"Collee JG, Fraser AG, Marmion BP, et al. (eds) (1996) Mackie & Mccartney Practical Medical Microbiology. 14th edition. New York: Churchill Livingstone.","edition":"14"},{"key":"e_1_3_9_8_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S360123"},{"key":"e_1_3_9_9_1","volume-title":"Product Information: MBPCR133, Revision No. 08","author":"HiMedia Laboratories Pvt. Ltd","year":"2025","unstructured":"HiMedia Laboratories Pvt. Ltd (2025) Hi-PCR\u00ae Methicillin Resistant Staphylococcus aureus (MRSA) (multiplex) probe PCR kit. In: Product Information: MBPCR133, Revision No. 08. Mumbai, India: HiMedia Laboratories Pvt. 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(2023) Prevalence of mupirocin- and methicillin-resistant Staphylococcus aureus in nasal carriage among healthcare workers in an intensive care unit and post-decolonization screening outcomes at a tertiary care hospital: a prospective study. 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