{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T07:14:27Z","timestamp":1793862867145,"version":"4.6.1"},"reference-count":23,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Therapeutic Advances in Musculoskeletal"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>Line immune-assays (LIA) for the detection of myositis-specific antibodies (MSA) are used widely for characterization of idiopathic inflammatory myopathies (IIM). Their current use and significance for the diagnosis of IIM remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>In this retrospective analysis, we retrieved clinical diagnoses of patients tested for MSA and myositis-associated antibodies (MAA) Jo-1, Mi-2\u03b1, Mi-2\u03b2, TIF1\u03b3, SRP, MDA-5, NXP-2, SAE, PL-7, PL-12, EJ, OJ, PM-Scl100, PM-Scl75 and Ku. We calculated clinical specificity, clinical sensitivity, negative- and positive predictive values (PPV) as well as positive and negative likelihood ratios.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>In total, we analyzed 3167 samples. After exclusion of repeated measurements and patients with insufficient clinical information, data of 1118 patients were available for analysis. A total of 242 patients tested positive for at least one antibody, of which 45 patients had a diagnosis of IIM; 25 IIM patients were negative for all MSA\/MAA. Clinical specificity of MSA\/MAA for the diagnosis of IIM ranged between 94.2% and 99.9%. Clinical sensitivity and PPV across all antibodies tested ranged from 0.0% to 12.9% and 0.0% to 72.7%, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>In clinical practice MSA\/MAA are used widely for diagnostic work-up of IIM, resulting in a low pre-test probability. Clinicians should be aware that PPVs for most MSA\/MAA are low.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/1759720x20975907","type":"journal-article","created":{"date-parts":[[2020,12,10]],"date-time":"2020-12-10T13:02:06Z","timestamp":1607605326000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":29,"title":["The use and diagnostic value of testing myositis-specific and myositis-associated autoantibodies by line immuno-assay: a retrospective study"],"prefix":"10.1177","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2036-9564","authenticated-orcid":false,"given":"Angelika","family":"Lackner","sequence":"first","affiliation":[{"name":"Division of Rheumatology and Immunology, Department of Internal Medicine, Medical University of Graz, Graz, Steiermark, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktoria","family":"Tiefenthaler","sequence":"additional","affiliation":[{"name":"Division of Rheumatology and Immunology, Department of Internal Medicine, Medical University of Graz, Graz, Steiermark, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jalia","family":"Mirzayeva","sequence":"additional","affiliation":[{"name":"Division of Rheumatology and Immunology, Department of Internal Medicine, Medical University of Graz, Graz, Steiermark, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Posch","sequence":"additional","affiliation":[{"name":"Division of Clinical Oncology, Department of Internal Medicine, Medical University Graz, Graz, Steiermark, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Rossmann","sequence":"additional","affiliation":[{"name":"Division of Clinical Oncology, Department of Internal Medicine, Medical University Graz, Graz, Steiermark, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kastriot","family":"Kastrati","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine III, Medical University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helga","family":"Radner","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine III, Medical University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"Demel","sequence":"additional","affiliation":[{"name":"Division of Rheumatology and Immunology, Department of Internal Medicine, Medical University of Graz, Graz, Steiermark, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Gretler","sequence":"additional","affiliation":[{"name":"Division of Rheumatology and Immunology, Department of Internal Medicine, Medical University of Graz, Graz, Steiermark, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Stotz","sequence":"additional","affiliation":[{"name":"Division of Clinical Oncology, Department of Internal Medicine, Medical University Graz, Graz, Steiermark, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Winfried B","family":"Graninger","sequence":"additional","affiliation":[{"name":"Division of Rheumatology and Immunology, Department of Internal Medicine, Medical University of Graz, Graz, Steiermark, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7884-6626","authenticated-orcid":false,"given":"Martin H","family":"Stradner","sequence":"additional","affiliation":[{"name":"Department of Rheumatology & Immunology, Medical University of Graz, Auenbruggerplatz 15, Graz, Steiermark 8036, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,12,8]]},"reference":[{"key":"bibr1-1759720X20975907","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2017-211468"},{"key":"bibr2-1759720X20975907","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2018.56"},{"key":"bibr3-1759720X20975907","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kew146"},{"key":"bibr4-1759720X20975907","doi-asserted-by":"publisher","DOI":"10.1111\/1756-185X.12792"},{"key":"bibr5-1759720X20975907","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2018-214690"},{"key":"bibr6-1759720X20975907","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2016.02.017"},{"key":"bibr7-1759720X20975907","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2018-213320"},{"key":"bibr8-1759720X20975907","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2017-212915"},{"key":"bibr9-1759720X20975907","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/key451"},{"key":"bibr10-1759720X20975907","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00412"},{"key":"bibr11-1759720X20975907","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00150-2015"},{"key":"bibr12-1759720X20975907","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2013-eular.3058"},{"key":"bibr13-1759720X20975907","first-page":"637","volume":"76","author":"O\u2019donnell 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