{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T12:12:11Z","timestamp":1784722331734,"version":"3.55.0"},"reference-count":28,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Suzhou Science and Technology Plan Project","award":["SZM2022012"],"award-info":[{"award-number":["SZM2022012"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cancer Biomarkers"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Colorectal cancer (CRC) remains a leading cause of cancer-related death worldwide. Although colonoscopy is effective screening method, its widespread adoption is hampered by poor compliance. Development of patient-friendly screening methods that are minimally invasive is of paramount importance to improve CRC screening participation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A single-center case-control study was conducted. Blood samples were collected from 119 participants (79 CRC patients, 20 patients with colorectal adenomas, and 20 healthy colonoscopy-negative controls) during May 2023 and March 2025. The methylated SEPT9 (mSEPT9) DNA in peripheral blood was analyzed by droplet digital polymerase chain reaction (ddPCR).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The sensitivity and specificity of mSEPT9 detection in peripheral blood for diagnosis of all stages of CRC patients was 68.35% and 95.00%, respectively. The positive rate were similar between left-sided CRC and right-sided CRC (65.31% vs 72.41%\uff0cp\u2009\uff1e\u20090.05). mSEPT9 detection in all healthy people with normal colonoscopy were negative. When mSEPT9 detection was combined with CEA and CA199, positive rate could be raised to 82.67%.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Blood-based mSEPT9 detection by ddPCR method is a sensitive and specific method for non-invasive diagnosis of CRC. Combination with CEA and CA19-9 could improve its performance. It may serve as a viable alternative for individuals unwilling or unable to undergo invasive procedures.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/18758592261449027","type":"journal-article","created":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T12:28:02Z","timestamp":1778156882000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection of methylated SEPT9 DNA in peripheral blood for diagnosis of colorectal cancer"],"prefix":"10.1177","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2377-1057","authenticated-orcid":false,"given":"Mengjie","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, The Fourth Affiliated Hospital of Soochow University (Suzhou Dushu Lake Hospital), Suzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of General Surgery, The Fourth Affiliated Hospital of Soochow University (Suzhou Dushu Lake Hospital), Suzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8274-7524","authenticated-orcid":false,"given":"Caifeng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Fourth Affiliated Hospital of Soochow University (Suzhou Dushu Lake Hospital), Suzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,5,7]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2021.101174"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2022-327736"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.2147\/CLEP.S391058"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2019.06.043"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.14309\/ajg.0000000000001122"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21457"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-010-9338-7"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2021.08.054"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1158\/2767-9764.CRC-21-0142"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.7754\/Clin.Lab.2023.230426"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-95712-5"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2024.117781"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2013.206375"},{"key":"e_1_3_2_16_2","first-page":"32","article-title":"Detection of methylated septin 9 DNA in blood for diagnosis, prognosis, and surveillance of colorectal cancer","volume":"25","author":"Leung WK","year":"2019","unstructured":"Leung WK, Shin VY, Law WL. Detection of methylated septin 9 DNA in blood for diagnosis, prognosis, and surveillance of colorectal cancer. Hong Kong Med J 2019; 25: 32\u201334.","journal-title":"Hong Kong Med J"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.acc.2015.07.004"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-03321-8"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2012-304149"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2013.221044"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2023.0425"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-02879-8"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2021.568818"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.14309\/ctg.0000000000000386"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-024-12670-3"},{"key":"e_1_3_2_26_2","first-page":"100748","article-title":"Methylated Septin9 identified patients with colorectal carcinoma and showed higher sensitivity than conventional biomarkers in detecting tumor","volume":"36","author":"Leerhoff S","year":"2023","unstructured":"Leerhoff S, Raem A, Kolbe EW, et al. Methylated Septin9 identified patients with colorectal carcinoma and showed higher sensitivity than conventional biomarkers in detecting tumor. 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