{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:36:31Z","timestamp":1787841391153,"version":"build-2784847793"},"reference-count":27,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T00:00:00Z","timestamp":1772668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100001671","name":"Hereditary Disease Foundation","doi-asserted-by":"publisher","award":["n\/a"],"award-info":[{"award-number":["n\/a"]}],"id":[{"id":"10.13039\/100001671","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2024-05769"],"award-info":[{"award-number":["RGPIN-2024-05769"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Schr\u00f6dinger, LLC","award":["n\/a"],"award-info":[{"award-number":["n\/a"]}]},{"name":"NIH\/ORIP","award":["R24OD037757"],"award-info":[{"award-number":["R24OD037757"]}]},{"DOI":"10.13039\/501100000024","name":"CIHR","doi-asserted-by":"crossref","award":["198025"],"award-info":[{"award-number":["198025"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007224","name":"Connaught Fund","doi-asserted-by":"publisher","award":["n\/a"],"award-info":[{"award-number":["n\/a"]}],"id":[{"id":"10.13039\/501100007224","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Huntington's Disease"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Reliable detection of huntingtin (HTT) is essential for understanding Huntington's disease (HD) biology and for evaluating therapeutic strategies. However, high-quality monoclonal antibodies (mAbs) against the HTT C-terminal domain remain limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>We sought to generate and validate novel monoclonal antibodies targeting the HTT C-terminal HEAT-containing domain to better detect HTT independently of potential effects of polyglutamine length that can impact some N-terminally targeted antibodies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We immunized mice with a highly purified, well-characterized recombinant protein corresponding to the HTT C-terminal domain. We generated monoclonal antibody-producing hybridoma cell lines and characterized the antibodies in common immuno-applications using parental and HTT-knockout cell lines, and in patient-derived fibroblasts.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Three novel, independent hybridoma lines producing anti-HTT monoclonal antibodies were derived. Using CRISPR-edited HTT knockout cell lines and in patient-derived cells, we identified one clone, anti-HTT [2F8], that was specific and effective across Western blot, immunofluorescence, and ELISA assays. All antibodies bound full-length HTT irrespective of HAP40 interaction or polyQ length in vitro and in cells and showed no cross-reactivity to the N-terminal HEAT domain.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These C-terminal HTT mAbs are thus valuable additional tools for studying endogenous HTT function in both normal and disease contexts.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/18796397261426634","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T18:31:37Z","timestamp":1773253897000},"page":"386-398","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Novel monoclonal antibodies against the C-terminal HEAT domain of huntingtin"],"prefix":"10.1177","volume":"15","author":[{"given":"Yang-Nim","family":"Park","sequence":"first","affiliation":[{"name":"The University of Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9995-5875","authenticated-orcid":false,"given":"Rebeka","family":"Fanti","sequence":"additional","affiliation":[{"name":"University of Toronto"},{"name":"University of Toronto"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6349-1638","authenticated-orcid":false,"given":"Samira","family":"Sadeghi","sequence":"additional","affiliation":[{"name":"University of Toronto"},{"name":"Department of Medical Biophysics, University of Toronto, Toronto, ON M5S 1A8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7593-2476","authenticated-orcid":false,"given":"Renu","family":"Chandrasekaran","sequence":"additional","affiliation":[{"name":"University of Toronto"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aled M","family":"Edwards","sequence":"additional","affiliation":[{"name":"University of Toronto"},{"name":"University of Toronto"},{"name":"Princess Margaret Cancer Centre"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel J","family":"Harding","sequence":"additional","affiliation":[{"name":"University of Toronto"},{"name":"Department of Pharmacology and Toxicology, Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada"},{"name":"Princess Margaret Cancer Centre"},{"name":"Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON M5S 3M2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7438-4655","authenticated-orcid":false,"given":"Douglas W","family":"Houston","sequence":"additional","affiliation":[{"name":"The University of Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,3,5]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.01.039"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90127-D"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2319091121"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21637-y"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00420-09"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2023.06.002"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac342"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-02895-4"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.153670.1"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.007204"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/mab.2015.0072"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-007-0346-8"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1089\/mab.2013.0089"},{"key":"e_1_3_3_15_2","first-page":"1","article-title":"Interdomain disulfide bonds of rabbit kappa light chain allotypes influence mouse-rabbit chimeric antibody performance","author":"Park Y-N","year":"2025","unstructured":"Park Y-N, Houston DW. 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